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Vector Backbone Description: Backbone Size:7473; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58548 Copy
Vector Backbone Description: Backbone Size:7912; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The sequence discrepancies between the full sequence and Addgene's QC sequence should not have any functional consequences.
Proper citation: RRID:Addgene_58556 Copy
Vector Backbone Description: Backbone Size:8299; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58557 Copy
Vector Backbone Description: Backbone Size:8098; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58550 Copy
Vector Backbone Description: Backbone Size:7084; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58552 Copy
Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: The V5 epitope tag serves as a linker between Dam and your favorite protein.
The lentiviral vectors can be used in two ways. When transfected as a conventional plasmid, the strong viral promoter drives high expression of the Dam(fusion) proteins. This should not be used for DamID experiments, but it is useful for checking of the proteins by Western blotting or immunofluorescence microscopy. When used as lentivirus, the strong promoter is deleted upon integration of the virus, and expression of the Dam(fusion) is now driven by the weak pIND-derived heatshock promoter.
Proper citation: RRID:Addgene_59205 Copy
Vector Backbone Description: Backbone Marker:van Steensal lab, Addgene plasmid 59201; Backbone Size:7660; Vector Backbone:pIND(V5)EcoDam; Vector Types:Mammalian Expression, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: Note that this vector can not be used to express Dam only.
Proper citation: RRID:Addgene_59204 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-cat
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59382 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-cat2
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. The 3'-end was modified from 5'-GGGCGGGGCGTAA to 5'-AGGAGGTGCATAA in order to provide a site resembling the consensus bacterial ribosomal binding site (5'-AGGAGGTAAATAA). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59385 Copy
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134979 Copy
Vector Backbone Description: Backbone Size:5288; Vector Backbone:pOO2-GW; Vector Types:bacterial expression vector for generating SP6 transcripts in vitro for Xenopus oocyte transfection; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21107422
Comments: The AtAMT1;1 coding sequence with the att sites (attB1 and attB2) from the pDRf1-AtAMT1;1 (Loque et al 2007 Nature 446 195-198) was remobilized with both restriction enzymes SpeI and XhoI then subsequently cloned between SpeI and XhoI sites in the p6013-002 (Ludewig et al., 2002 J. Biol. Chem. 277, 13548-13555). The AtAMT1;1 coding sequence was then replaced by the Gateway DNA cassette after a BP reaction with pDONR 221 (GATEWAY technology, Invitrogen) to generate the p002-GW vector.
Proper citation: RRID:Addgene_135097 Copy
Species: venezuelan equine encephalitis
Genetic Insert: Lvl2 (nsP2 Q739L)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115960 Copy
Species: Synthetic
Genetic Insert: Lvl0 SGP (+/- Apt) Dest
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115950 Copy
Species: Synthetic
Genetic Insert: Lvl0 ORF Dest
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115951 Copy
Vector Backbone Description: Backbone Size:7636; Vector Backbone:pMH-EBFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_161714 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161882 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161885 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161884 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161886 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161889 Copy
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