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Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182172 Copy
Species: Other
Genetic Insert: DsRed
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182171 Copy
Species: Other
Genetic Insert: DsRed
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182173 Copy
Species: phage phiC31
Genetic Insert: phiC31 attB site
Vector Backbone Description: Backbone Size:4670; Vector Backbone:pDEST; Vector Types:Drosophila transgenesis; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector with phiC31 attB site, for Drosophila transformation into attP landing sites.
Proper citation: RRID:Addgene_30325 Copy
Species: Homo sapiens
Genetic Insert: CD4-tdTom
Vector Backbone Description: Backbone Size:10193; Vector Backbone:pAPIC-HIH; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21606367
Comments: Gateway destination vector for cloning any enhancer to drive expression of membrane marker CD4-tdTom
The depositing laboratory recommends growing bacteria either on LB plates with 80 ug/ml Carbenicillin or in LB liquid media with 60 ug/ml Carbenicillin (a semi-synthetic ampicillin analog)
Proper citation: RRID:Addgene_31222 Copy
Vector Backbone Description: Backbone Size:5808; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette
Proper citation: RRID:Addgene_22423 Copy
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pCS2+; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: pCSDest was digested with PstI and XhoI to remove the attR2 site and was replaced with an attR3 site from pDESTR4-R3
Proper citation: RRID:Addgene_22424 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:13170; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16945906
Comments: Parent lentivector, co-expression of Venus selection gene
Proper citation: RRID:Addgene_25734 Copy
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:12944; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16945906
Comments: Parent lentivector, co-expression of Zeocin selection gene
Proper citation: RRID:Addgene_25736 Copy
Vector Backbone Description: Backbone Size:9377; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21706014
Comments: There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.
Proper citation: RRID:Addgene_25890 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25946 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25943 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25945 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25941 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25959 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25960 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25962 Copy
Vector Backbone Description: Backbone Size:7401; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for puromycin selection of transgenes in C. elegans.
This vector is identical to pBCN21-R4R3, but a 8xC183_minPmyo-2::mCherry::unc-54_3'UTR cassette was inserted in the backbone as an additional visual marker.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26302 Copy
Vector Backbone Description: Backbone Size:8090; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for puromycin selection of transgenes in Caenorhabditis species other than C. elegan (works also in C. elegans).
This vector is the same as pBCN22-R4R3 but with a different Pmyo-2::mCherry::unc-54_3'UTR construct that gives good fluoresence expression in the pharynx of C. elegans as well as other species, e.g. C. briggsae, C. remanei and C. brenneri.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4::VP16
Vector Backbone Description: Backbone Size:8770; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26228 Copy
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