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Vector Backbone Description: Vector Backbone:DVC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39801078
Proper citation: RRID:Addgene_217600 Copy
Vector Backbone Description: Vector Backbone:DVC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39801078
Proper citation: RRID:Addgene_217601 Copy
Species: Homo sapiens
Genetic Insert: CAMK2D
Vector Backbone Description: Vector Backbone:pLenti-C-myc-DDK-P2A-Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37468549
Comments: This is a mutated version of plasmid #221699.
Proper citation: RRID:Addgene_221700 Copy
Species: Homo sapiens
Genetic Insert: CAMK2D
Vector Backbone Description: Vector Backbone:pLenti-C-myc-DDK-P2A-Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37468549
Proper citation: RRID:Addgene_221699 Copy
Vector Backbone Description: Vector Backbone:DVC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39801078
Proper citation: RRID:Addgene_217588 Copy
Vector Backbone Description: Vector Backbone:DVC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39801078
Proper citation: RRID:Addgene_217597 Copy
Vector Backbone Description: Vector Backbone:DVC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39801078
Proper citation: RRID:Addgene_217598 Copy
Vector Backbone Description: Vector Backbone:DVC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39801078
Proper citation: RRID:Addgene_217599 Copy
Species: Escherichia coli
Genetic Insert: NsrR promoter only
Vector Backbone Description: Vector Backbone:pBAC (Addgene Plasmid #13423); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38687030
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.28.568972v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_220162 Copy
Species: Escherichia coli
Genetic Insert: SoxS promoter only
Vector Backbone Description: Vector Backbone:pBAC (Addgene Plasmid #13423); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38687030
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.28.568972v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_220165 Copy
Species: Escherichia coli
Genetic Insert: Cra promoter only
Vector Backbone Description: Vector Backbone:pBAC (Addgene Plasmid #13423); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38687030
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.28.568972v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_220151 Copy
Species: Escherichia coli
Genetic Insert: CRP promoter only
Vector Backbone Description: Vector Backbone:pBAC (Addgene Plasmid #13423); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38687030
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.28.568972v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_220152 Copy
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:2000; Vector Backbone:pZ; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36384003
Proper citation: RRID:Addgene_205225 Copy
Vector Backbone Description: Backbone Size:5256; Vector Backbone:pSpy0C4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white.
Resource Information: The MCS featuring the Plac_mrfp cassette and the chloramphenicol resistance cassette were taken from pSpy1C. The pUC origin for replication in E. coli was taken from pUC19-mKate2∼ssrA. Up- and downstream homologous regions of sagB were amplified from the S. pyogenes SF370 genome.
Linearise the plasmid with the restriction enzyme Alw44I before transformation in S. pyogenes to favour double crossover recombination of the plasmid into the sagB locus. Positive transformants will show loss of hemolysis on TSA blood agar plates.
Please download the file linked below to obtain a well annotated map of the plasmid.
Proper citation: RRID:Addgene_220280 Copy
Species: Photinus pyralis
Genetic Insert: ffluc
Vector Backbone Description: Vector Backbone:pSpy1C; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: This high-copy replicative shuttle plasmid features an erythromycin-inducible riboswitch driving expression of the Firefly luciferase in the presence of erythromycin. Luminescence can be detected by adding luciferin to the culture. See associated publication for more details.
Resource Information: The erythromycin-inducible riboswitch was taken from pLZ12Km2-TA:PgyrA(Sag)-ermBL-ermB':A2I:ffluc-tt3, which was used in the following publication:
Dynamics of diversified A-to-I editing in Streptococcus pyogenes is governed by changes in mRNA stability
Thomas F. Wulff, Karin Hahnke, Anne-Laure Lécrivain, Katja Schmidt, Rina Ahmed-Begrich, Knut Finstermeier, Emmanuelle Charpentier
bioRxiv 2023.09.19.555891; doi: https://doi.org/10.1101/2023.09.19.555891
The ffluc reporter gene was taken from the original pLZ12Km2-P23R:TA:ffluc plasmid (Plasmid #88900).
Please download the detailed plasmid map using the file linked below
Proper citation: RRID:Addgene_218513 Copy
Vector Backbone Description: Backbone Size:4917; Vector Backbone:pSpy1C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white.
Resource Information: We took the rolling circle replicon pSH71 and the chloramphenicol resistance cassette from pIB166 (Plasmid #90189). The the Plac_mrfp cassette for the MCS is derived from pBS3Clux (Plasmid #55172).
Please download the file linked below to obtain a well annotated map of the plasmid.
Proper citation: RRID:Addgene_220248 Copy
Species: Synthetic
Genetic Insert: LCB3_v1.2
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194228 Copy
Species: Homo sapiens
Genetic Insert: EF1a Promoter
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194225 Copy
Species: Synthetic
Genetic Insert: LCB1_v1.3
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194227 Copy
Vector Backbone Description: Backbone Size:2340; Vector Backbone:pSU18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39244484
Comments: Mid copy plasmid (p15a ori) with pUC18 MCS with FLAG epitope integrated at SmaI site, confers chloramphenicol resistance
Proper citation: RRID:Addgene_225155 Copy
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