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Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5139; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200999 Copy
Genetic Insert: dCas9, sgRNA expression cassette, sfGFP
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38126769
Proper citation: RRID:Addgene_208889 Copy
Genetic Insert: dCas9, sgRNA expression cassette
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38126769
Proper citation: RRID:Addgene_208890 Copy
Species: Homo sapiens
Genetic Insert: AEBP2LTEV
Vector Backbone Description: Vector Backbone:pMB.Pos5; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247016 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247004 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1∆
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247008 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Vector Backbone:pBIG1a; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247015 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt2K
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247010 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt2∆
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247011 Copy
Species: Synthetic
Genetic Insert: UNS1-P6d-U3d-C7d-t0-UNSX
Vector Backbone Description: Vector Backbone:EML002; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40998411
Comments: Please visit https://doi.org/10.1101/2025.06.09.658710 for bioRxiv preprint.
Proper citation: RRID:Addgene_238408 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Vector Backbone:pMB.Pos5; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247017 Copy
Species: Synthetic
Genetic Insert: SpyTag-noro-VLP
Vector Backbone Description: Backbone Marker:Gibco; Backbone Size:5238; Vector Backbone:pFastBac Dual; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:33468139
Comments: Original publication:
SpyCatcher:
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. PMID: 22366317; PMCID: PMC3311370.
Proper citation: RRID:Addgene_165989 Copy
Vector Backbone Description: Backbone Size:5279; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:17117155
Comments: pFBDM was derived from pFastBacDUAL (Invitrogen), which enables the production of recombinant proteins in insect cells by baculovirus. This pFBDM vector is used mostly in our lab for the production of various target proteins composed of one or two polypeptides.
Proper citation: RRID:Addgene_110738 Copy
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