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Species: E. coli
Genetic Insert: MetN
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118257 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118259 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118256 Copy
Species: E. coli
Genetic Insert: LexAp65
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR P5-P2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_41437 Copy
Species: E. coli
Genetic Insert: LplA(AILR) with ER-retention sequence KDEL
Vector Backbone Description: Backbone Size:4261; Vector Backbone:pCAG; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23181687
Proper citation: RRID:Addgene_42574 Copy
Species: E. coli
Genetic Insert: EcoRI endonuclease & EcoRI methylase
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pKC30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6088551
Comments: pKC30 plasmid derivation is described in Nature (1981) 292, 128-132.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_39993 Copy
Species: E. coli
Genetic Insert: gusA
Vector Backbone Description: Vector Backbone:RP4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:22820336
Comments: GenBank accession number JQ895026
Multiple-cloning site (MCS) of pLMB51: GATTACCTCA GTCTAGAGCT CGAGAAGCTT GGATCCATGG TACCCTACA
Proper citation: RRID:Addgene_40083 Copy
Species: E. coli
Genetic Insert: GFP-LacI**
Vector Backbone Description: Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:4600; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21724830
Comments: The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser).
The plasmid pAT222 (Addgene plasmid #40942) was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase):
5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and
5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’.
The LacI** variant was then subcloned into pAFS135 using NotI and XhoI restriction sites to create plasmid pAT253.
Proper citation: RRID:Addgene_40943 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:InVitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22412018
Proper citation: RRID:Addgene_35700 Copy
Species: E. coli
Genetic Insert: BirA(R118G)-HA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5333; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22412018
Proper citation: RRID:Addgene_36047 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164601 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164602 Copy
Species: E. Coli
Genetic Insert: ble
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165840 Copy
Species: E. Coli
Genetic Insert: nptII
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165836 Copy
Species: E. Coli
Genetic Insert: hph
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165839 Copy
Species: E. Coli
Genetic Insert: pac
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165837 Copy
Species: E. Coli
Genetic Insert: rrnBT1
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Bacterial Terminator
Proper citation: RRID:Addgene_165803 Copy
Species: E. Coli
Genetic Insert: rrnBT2
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Bacterial Terminator
Proper citation: RRID:Addgene_165804 Copy
Species: E. coli
Genetic Insert: sgNeo/sgNT1
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177211 Copy
Species: E. coli
Genetic Insert: sgNeo1/sgNT1/sgNeo2
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177231 Copy
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