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Species: Synthetic
Genetic Insert: L13-9
Vector Backbone Description: Backbone Size:6008; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242352 Copy
Species: Synthetic
Genetic Insert: L15-20
Vector Backbone Description: Backbone Size:6008; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242353 Copy
Species: Synthetic
Genetic Insert: 5'UTR-bpNLS-BxbI-3'UTR-polyA
Vector Backbone Description: Backbone Marker:Addgene #207455; Vector Backbone:IVT-nCas9(H840A); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248216 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Size:5058; Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242356 Copy
Species: Homo sapiens
Genetic Insert: tet3G-cmv-FRB-SMG7C-IRES-FKBP-HaloTag-tdMCP-NLS-HA
Vector Backbone Description: Backbone Size:8545; Vector Backbone:PSR1903 tet3G; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38548718
Proper citation: RRID:Addgene_232461 Copy
Species: Homo sapiens
Genetic Insert: PERIOD 2 Promoter
Vector Backbone Description: Backbone Marker:Alexeyev Lab; Backbone Size:8331; Vector Backbone:pMA3160; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41226820
Comments: For generation of PER2-luciferase (PER2-Luc) plasmid, a 952-bp EcoRI/NotI fragment was isolated from a pGL3 basic PER2 construct, obtained from Dr. Helmut Zarbl (Rutgers University). The PER2 promoter-containing fragment was subcloned into the lentiviral construct pMA3160 (Addgene plasmid #35043, deposited by Dr Mikhail Alexeyev) to generate a PER2-luciferase reporter construct.
Proper citation: RRID:Addgene_250937 Copy
Species: SARS-CoV-2
Genetic Insert: Fragment D2 SARS-CoV-2 ΔE-GFPΔ6Δ8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253121 Copy
Species: SARS-CoV-2
Genetic Insert: Fragment D2 SARS-CoV-2 ΔEΔ6Δ7 orf3*
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253122 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2_frD2_ΔORF7a
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253126 Copy
Genetic Insert: PE7 C-terminal
Vector Backbone Description: Vector Backbone:puc19; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42127219
Proper citation: RRID:Addgene_251106 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2_frD2_ΔORF7b
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253127 Copy
Species: Synthetic
Genetic Insert: 5'UTR-PE7-3'UTR-polyA
Vector Backbone Description: Backbone Marker:Addgene #207455; Vector Backbone:IVT-nCas9(H840A); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248222 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB
Vector Backbone Description: Backbone Marker:Addgene #139987; Vector Backbone:pCMV-T7-SpCas9-P2A-EGFP (RTW3027); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248223 Copy
Species: Homo sapiens
Genetic Insert: PGE2 sensor GRAB_PGE2-1.0
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5119; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41831442
Comments: Please visit https://doi.org/10.1101/2025.06.01.657218 for the bioRxiv preprint.
Proper citation: RRID:Addgene_251355 Copy
Species: Synthetic
Genetic Insert: 5'UTR-SuperDn29-bpNLS-3'UTR-polyA
Vector Backbone Description: Backbone Marker:Addgene #207455; Vector Backbone:IVT-nCas9(H840A); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248220 Copy
Species: Synthetic
Genetic Insert: SacINT attP
Vector Backbone Description: Backbone Marker:Addgene #139987; Vector Backbone:pCMV-T7-SpCas9-P2A-EGFP (RTW3027); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248227 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2_frD2_ΔORF67a8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253130 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2_frD_ORF6-BirA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253132 Copy
Species: Homo sapiens
Genetic Insert: UBAP2L Nterm Guide RNA 1
Vector Backbone Description: Backbone Size:8484; Vector Backbone:pX333; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246894 Copy
Species: Homo sapiens
Genetic Insert: Caprin1 Nterm Guide RNA 1
Vector Backbone Description: Backbone Size:8484; Vector Backbone:pX332; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246893 Copy
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