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Species: S. pyogenes
Genetic Insert: dCas9 (bacteria)
Vector Backbone Description: Vector Backbone:SC101; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155371 Copy
Species: S. pyogenes
Genetic Insert: dCas9 (bacteria)
Vector Backbone Description: Vector Backbone:SC101; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155372 Copy
Species: Homo sapiens
Genetic Insert: Ash2L
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Comments: Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'.
Proper citation: RRID:Addgene_15549 Copy
Species: Homo sapiens
Genetic Insert: Ash2L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Comments: Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'
Please note that mutation R622H in ASH2L was found during Addgene's quality control. The plasmid should function as described.
Proper citation: RRID:Addgene_15548 Copy
Species: Mus musculus
Genetic Insert: Tbr1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:E61 (pBS derivative); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7619531
Comments: To make antisense probe, cut with HindIII and transcribe with T3 RNA polymerase.
Proper citation: RRID:Addgene_15543 Copy
Species: Mus musculus
Genetic Insert: Nkx6.2
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9533954
Comments: To make antisense probe, cut with BamHI and transcribe with T7 RNA polymerase.
Proper citation: RRID:Addgene_15542 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: dCas12a (F. novicida)
Vector Backbone Description: Vector Backbone:SC101; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155382 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: dCas12a (F. novicida)
Vector Backbone Description: Vector Backbone:SC101; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155384 Copy
Species: Synthetic
Genetic Insert: PphlF-TA4 in dCas12a oscillator crRNAs
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155385 Copy
Species: Synthetic
Genetic Insert: dCas12a oscillator crRNAs
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155381 Copy
Species: Dengue virus 4
Genetic Insert: DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4779; Vector Backbone:pProExHTb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32763315
Comments: original plasmid received from SV lab.
Proper citation: RRID:Addgene_155317 Copy
Species: Homo sapiens
Genetic Insert: htt 103Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Met; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 1C in article.
Lindquist lab number: 8056
Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.
Proper citation: RRID:Addgene_15591 Copy
Species: Synthetic
Genetic Insert: PfEBA175
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32888502
Proper citation: RRID:Addgene_155312 Copy
Species: Dengue virus 4
Genetic Insert: Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4779; Vector Backbone:pNIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32763315
Proper citation: RRID:Addgene_155320 Copy
Species: Homo sapiens
Genetic Insert: htt 103Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 2, VI in article.
Lindquist lab number: 7704
Proper citation: RRID:Addgene_15579 Copy
Species: Homo sapiens
Genetic Insert: htt 25Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 2, VI in article.
Lindquist lab number: 7703
Proper citation: RRID:Addgene_15578 Copy
Vector Backbone Description: Backbone Size:6866; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.57, Ligation number L3567.
Proper citation: RRID:Addgene_1559 Copy
Species: Homo sapiens
Genetic Insert: htt25Q + Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 1, I in article.
Lindquist lab number: 7570
Proper citation: RRID:Addgene_15574 Copy
Species: Homo sapiens
Genetic Insert: htt 25Q + Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 2, V in article.
Lindquist lab number: 7699
Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.
Proper citation: RRID:Addgene_15570 Copy
Vector Backbone Description: Backbone Size:6866; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.50, Ligation number L3562.
Proper citation: RRID:Addgene_1554 Copy
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