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Vector Backbone Description: Vector Backbone:PZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41967643
Proper citation: RRID:Addgene_254183 Copy
Species: Homo sapiens
Genetic Insert: L1RP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:puc19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255271 Copy
Species: Staphylococcus aureus
Genetic Insert: dSaCas9-KRAB(Zim3)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255031 Copy
Species: Synthetic
Genetic Insert: RfxCas13d/CasRx
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255033 Copy
Species: West Nile Virus
Genetic Insert: NS2B-NS3 protease
Vector Backbone Description: Backbone Marker:Novagen/NESG; Backbone Size:5681; Vector Backbone:pET15SUMO2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_240392 Copy
Species: Synthetic
Genetic Insert: LiON (Labile iron and Oxygen Notifier)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42105746
Comments: This plasmid is also associated with the following publication:
Maeda A, Moroishi T. (2026) Protocol to assess intracellular labile iron and oxygen at single-cell resolution using a genetically encoded ratiometric reporter. STAR Protocols, 2026; 7.
PMID: 42118663. DOI: https://doi.org/10.1016/j.xpro.2026.104561
Proper citation: RRID:Addgene_252093 Copy
Species: Rattus norvegicus
Genetic Insert: CLIP-170 CAP-Gly
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41662400
Proper citation: RRID:Addgene_255801 Copy
Species: Triticum aestivum and Triticum turgidum
Genetic Insert: SpCas9-P2A-RUBY
Vector Backbone Description: Vector Backbone:JD633; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2026.02.10.705172 for bioRxiv preprint.
The reference articles to construct this plasmid:
A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. 10.1038/s41587-020-0703-0 PubMed 33046875
A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566
Proper citation: RRID:Addgene_253105 Copy
Species: Synthetic
Genetic Insert: A1aY1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41662400
Proper citation: RRID:Addgene_255800 Copy
Species: Triticum aestivum
Genetic Insert: SpCas9-P2A-RUBY
Vector Backbone Description: Vector Backbone:JD633; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2026.02.10.705172 for bioRxiv preprint.
The reference articles to construct this plasmid:
A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. 10.1038/s41587-020-0703-0 PubMed 33046875
A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566
Proper citation: RRID:Addgene_253106 Copy
Vector Backbone Description: Backbone Size:16816; Vector Backbone:pGMV-U; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_251731 Copy
Species: Synthetic
Genetic Insert: L13-23
Vector Backbone Description: Backbone Size:2762; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242348 Copy
Species: Synthetic
Genetic Insert: wild-type class B tetracycline repressor
Vector Backbone Description: Backbone Size:2762; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242345 Copy
Species: Synthetic
Genetic Insert: L12-11
Vector Backbone Description: Backbone Size:2762; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42259793
Proper citation: RRID:Addgene_242346 Copy
Genetic Insert: prime editing guideRNA
Vector Backbone Description: Vector Backbone:sgOpti; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40245860
Proper citation: RRID:Addgene_255532 Copy
Species: E. coli
Genetic Insert: dnaX
Vector Backbone Description: Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The Ec dnaX gene native sequence, which is in this plasmid, has a frameshift that happens with approximately 50% efficiency, resulting in approximately equal expression of the gamma and tau subunits of E. coli DNA Polymerase III from this clone.
Proper citation: RRID:Addgene_255907 Copy
Species: SARS-CoV-2, Influenza virus PR8
Genetic Insert: CoV-2 frD with Influenza N1 PR8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: detailed explanation of CLEVER reverse genetics methodology in DOI: 10.7554/eLife.89035
Proper citation: RRID:Addgene_253169 Copy
Species: Homo sapiens
Genetic Insert: ANKRD11
Vector Backbone Description: Vector Backbone:pCAG-puro-3xFLAG (DEST); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40520101
Proper citation: RRID:Addgene_236621 Copy
Species: Salmonella typhimurium
Genetic Insert: 2-deoxyribose-5-phosphate aldolase (DERA) from Salmonella typhimurium
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET 15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31806708
Proper citation: RRID:Addgene_255517 Copy
Species: Moloney murine leukemia virus
Genetic Insert: MMLV-RT
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4723; Vector Backbone:pQE-81L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_252404 Copy
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