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Species: E. coli
Genetic Insert: neoPheS
Vector Backbone Description: Backbone Size:3470; Vector Backbone:pPB_LR5; Vector Types:Mammalian Expression, piggyBac transposon Gateway destination vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30477585
Proper citation: RRID:Addgene_112923 Copy
Species: Gallus gallus
Genetic Insert: OVA/p2a/mCherry-CaaX
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29862966
Proper citation: RRID:Addgene_113030 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-GCN4
Vector Backbone Description: Backbone Size:4968; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29875396
Proper citation: RRID:Addgene_113026 Copy
Species: Rattus norvegicus
Genetic Insert: scFv-rAPOBEC1-UGI-GB1
Vector Backbone Description: Backbone Size:4968; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29875396
Proper citation: RRID:Addgene_113029 Copy
Species: Homo sapiens
Genetic Insert: CD19 CAR-P intracellular GFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29862966
Proper citation: RRID:Addgene_113014 Copy
Species: Synthetic
Genetic Insert: AT1R
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29505611
Proper citation: RRID:Addgene_112934 Copy
Species: Danio rerio
Genetic Insert: IgM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3948; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15685175
Comments: Clone A1 (exon 1-3) was amplified using primers zfCH1 (5'-TCAACCATCTGCGCCCCAGT) and cm3A (5'-TCCTGAGGCTTTGCACTT).
Proper citation: RRID:Addgene_11301 Copy
Species: Synthetic
Genetic Insert: His6-SpyCatcher-mi3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5254; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30028591
Proper citation: RRID:Addgene_113043 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based DA sensor GRAB_DA1m
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4495; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30007419
Proper citation: RRID:Addgene_113049 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:N.A.; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29982721
Comments: Please visit https://www.biorxiv.org/content/early/2018/02/26/272377 for bioRxiv preprint.
Proper citation: RRID:Addgene_113150 Copy
Genetic Insert: CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377362
Comments: Addgene QC sequence analysis identified a mutation within NeoR/KanR, which results in an early stop codon. The effect of this mutation has not been determined experimentally. Ampicillin should be used for selection in bacteria.
Proper citation: RRID:Addgene_113035 Copy
Genetic Insert: 2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
Vector Backbone Description: Vector Backbone:scAAV vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377362
Comments: Addgene Next-Generation Sequence analysis identified numerous sequence discrepancies in ITR sequences. Plasmid is expected to function as described in the associated publication: PMID: 30377362
Proper citation: RRID:Addgene_113039 Copy
Genetic Insert: SV40 NLS-AcrIIA4
Vector Backbone Description: Vector Backbone:scAAV vector; Vector Types:Mammalian Expression, AAV, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377362
Comments: Addgene Next-Generation Sequence analysis identified numerous sequence discrepancies in ITR sequences. Plasmid is expected to function as described in the associated publication: PMID: 30377362
Proper citation: RRID:Addgene_113038 Copy
Genetic Insert: 4 bulged binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two original binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away.
Flanking CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The inner binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11313 Copy
Species: Homo sapiens
Genetic Insert: p21 Activated kinase 4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29983868
Proper citation: RRID:Addgene_113088 Copy
Genetic Insert: 1 perfect binding site for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-control; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12600936
Comments: The end of the ORF and the beginning of the 3' UTR is (with the TAA
stop codon, followed by T, then the XbaI site TCTAGA):
GATCGCCGTG TAA T TCTAGA GTCGGGGCG.
We digested the plasmids with XbaI, and ligated in the following:
ctaga ttccgagatatcggtaat gggcc.
This produced a modified vector, still containing an XbaI site, but
now also containing an ApaI site (GGGCCC), to allow for directional
cloning of 3' UTR inserts. The final vector contains, starting with
the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA.
All UTR inserts were then constructed in the following format:
TCTAGACTCGAGCCGG[binding site]ATCGCGGGCCC.
A single perfect binding site is AAGTTTTCACTCCAGCTAACACCGG.
The CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU.
Proper citation: RRID:Addgene_11310 Copy
Species: Synthetic
Genetic Insert: GINKO1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30652129
Proper citation: RRID:Addgene_113112 Copy
Species: Homo sapiens
Genetic Insert: RapGEF3
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11801596
Proper citation: RRID:Addgene_113110 Copy
Species: Mus musculus
Genetic Insert: Gata3 donor region including homology arms and T2A-H2B-eGFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113119 Copy
Vector Backbone Description: Vector Backbone:T444T; Vector Types:Bacterial Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29924347
Comments: For plasmid usage, please see the associated publication: Sturm et al.
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Nucleic Acids Research, gky516, https://doi.org/10.1093/nar/gky516
Proper citation: RRID:Addgene_113082 Copy
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