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Species: Arabidopsis thaliana
Genetic Insert: TALE and DddAtox and TadA 8e
Vector Backbone Description: Vector Backbone:pBAtC; Vector Types:Plant Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36456803
Proper citation: RRID:Addgene_189643 Copy
Species: Arabidopsis thaliana
Genetic Insert: TALE and DddAtox
Vector Backbone Description: Vector Backbone:pBAtC; Vector Types:Plant Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36456803
Proper citation: RRID:Addgene_189644 Copy
Species: Arabidopsis thaliana
Genetic Insert: temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet ; Backbone Size:2073; Vector Backbone:pAGM3946; Vector Types:CRISPR, Synthetic Biology, MoClo compatible Level 0 module ; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36382936
Proper citation: RRID:Addgene_192453 Copy
Species: Arabidopsis thaliana
Genetic Insert: phytochrome interacting factor 3
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pL1A-lc; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28911120
Proper citation: RRID:Addgene_98744 Copy
Species: Arabidopsis thaliana
Genetic Insert: AFB2
Vector Backbone Description: Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Digest vector with EcoRV for integration into TRP1.
Proper citation: RRID:Addgene_99531 Copy
Species: Arabidopsis thaliana
Genetic Insert: TIR1
Vector Backbone Description: Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Digest this vector with EcoRV for integration into TRP1.
Addgene NGS results found a single nucleotide change compared to NM_116163.4 that results in T591I within the TIR1 translation. The protein is noted by the depositing laboratory to be functional in vivo
Proper citation: RRID:Addgene_99532 Copy
Species: Arabidopsis thaliana
Genetic Insert: IAA17(71-114)
Vector Backbone Description: Vector Backbone:pFA6a derivative; Vector Types:PCR template for yeast tagging cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23836714
Comments: Caution: internal inversion within the IAA17 sequence (aa89-104)
Proper citation: RRID:Addgene_99515 Copy
Species: Arabidopsis thaliana
Genetic Insert: IAA17(1-116)
Vector Backbone Description: Vector Backbone:pFA6a derivative; Vector Types:PCR template for yeast tagging cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23836714
Proper citation: RRID:Addgene_99516 Copy
Species: Arabidopsis thaliana
Genetic Insert: IAA17(71-116)
Vector Backbone Description: Vector Backbone:pFA6a derivative; Vector Types:PCR template for yeast tagging cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23836714
Proper citation: RRID:Addgene_99519 Copy
Species: Arabidopsis thaliana
Genetic Insert: IAA17(71-116)
Vector Backbone Description: Vector Backbone:pRS306 derivative; Vector Types:PCR template for yeast tagging cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23836714
Proper citation: RRID:Addgene_99526 Copy
Species: Arabidopsis thaliana
Genetic Insert: IAA17(71-117)
Vector Backbone Description: Vector Backbone:pRS306 derivative; Vector Types:PCR template for yeast tagging cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23836714
Proper citation: RRID:Addgene_99525 Copy
Species: Arabidopsis thaliana
Genetic Insert: IAA17(71-117)
Vector Backbone Description: Vector Backbone:pRS306 derivative; Vector Types:PCR template for yeast tagging cassette; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_99529 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUbi10 promoter
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:pMC-1-ccdB-6; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36896468
Comments: Please visit https://doi.org/10.1101/2022.10.15.511792 for bioRxiv preprint.
Proper citation: RRID:Addgene_197700 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtU6 promoter for sgRNA assembly
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:pMC-1-ccdB-11; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36896468
Comments: Please visit https://doi.org/10.1101/2022.10.15.511792 for bioRxiv preprint.
Proper citation: RRID:Addgene_197779 Copy
Species: Arabidopsis thaliana
Genetic Insert: Intermediate fragment for assembly of gRNA-TLS2 construct
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593415
Comments: Please refer to the GO CRISP Cloning Protocol linked above for additional information.
Proper citation: RRID:Addgene_196983 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtNIA1 gRNAs fused to TLS1 tags
Vector Backbone Description: Vector Backbone:pMDC100; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593415
Comments: Please refer to the GO CRISP Cloning Protocol linked above for additional information.
Proper citation: RRID:Addgene_196980 Copy
Species: Arabidopsis thaliana
Genetic Insert: gRNA expression cassette in pENTR4
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593415
Comments: Please refer to the GO CRISP Cloning Protocol linked above for additional information.
Proper citation: RRID:Addgene_198729 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtNIA1 gRNAs
Vector Backbone Description: Vector Backbone:pMDC7; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593415
Comments: Please refer to the GO CRISP Cloning Protocol linked above for additional information.
Proper citation: RRID:Addgene_198728 Copy
Species: Arabidopsis thaliana
Genetic Insert: SDT
Vector Backbone Description: Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27871334
Proper citation: RRID:Addgene_87934 Copy
Species: Arabidopsis thaliana
Genetic Insert: pAT5G42470
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet Lab; Vector Backbone:pICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37901686
Proper citation: RRID:Addgene_205385 Copy
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