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Species: Rattus norvegicus
Genetic Insert: Parkin 219-465
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23661642
Proper citation: RRID:Addgene_45972 Copy
Species: Rattus norvegicus
Genetic Insert: Parkin F146A
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23661642
Proper citation: RRID:Addgene_45973 Copy
Species: Rattus norvegicus
Genetic Insert: Parkin F463A
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23661642
Proper citation: RRID:Addgene_45975 Copy
Species: Rattus norvegicus
Genetic Insert: Parkin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23661642
Proper citation: RRID:Addgene_45969 Copy
Species: Rattus norvegicus
Genetic Insert: Jag1
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pT3-EF1a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23419361
Comments: This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.
Proper citation: RRID:Addgene_46051 Copy
Species: Rattus norvegicus
Genetic Insert: β-arrestin 1 S412D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Comments: Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing.
Proper citation: RRID:Addgene_42196 Copy
Species: Rattus norvegicus
Genetic Insert: ankyrin G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21551097
Proper citation: RRID:Addgene_42566 Copy
Species: Rattus norvegicus
Genetic Insert: Red Fluorescent Calcium binding Protein
Vector Backbone Description: Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2753; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23459413
Comments: Gene was cloned using BaMHI and HindIII - But part of the vector backbone then becomes part of the protein (leader sequence containing histag) - using NdeI and HindIII will completely remove the open reading frame/gene.
Proper citation: RRID:Addgene_42874 Copy
Species: Rattus norvegicus
Genetic Insert: Regulator of G Protein Signaling 4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9294233
Proper citation: RRID:Addgene_31157 Copy
Species: Rattus norvegicus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822278
Comments: Contains NMHTG linker between myc and Strep tags.
The hygromycin resistance gene in this plasmid lacks a promoter and native start codon. See Invitrogen's website for more information about the vector backbone.
Proper citation: RRID:Addgene_31840 Copy
Species: Rattus norvegicus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822278
Comments: Contains NMHTG linker between myc and Strep tags.
The hygromycin resistance gene in this plasmid lacks a promoter and native start codon. See Invitrogen's website for more information about the vector backbone.
Proper citation: RRID:Addgene_31839 Copy
Species: Rattus norvegicus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822278
Comments: Contains NMHTG linker between myc and Strep tags.
The hygromycin resistance gene in this plasmid lacks a promoter and native start codon. See Invitrogen's website for more information about the vector backbone.
Proper citation: RRID:Addgene_31838 Copy
Species: Rattus norvegicus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822278
Comments: Contains NMHTG linker between myc and Strep tags.
The hygromycin resistance gene in this plasmid lacks a promoter and native start codon. See Invitrogen's website for more information about the vector backbone.
Proper citation: RRID:Addgene_31837 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP5G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23035093
Proper citation: RRID:Addgene_31788 Copy
Species: Rattus norvegicus
Genetic Insert: Elongin B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5070; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18187417
Proper citation: RRID:Addgene_31983 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18460329
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32451 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32456 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32457 Copy
Species: Rattus norvegicus
Genetic Insert: Constitutively active Rheb (S16H)
Vector Backbone Description: Backbone Marker:Addgene plasmid #32519; Vector Backbone:pHAGE-CMV-Rheb-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20062052
Comments: The constitutively activating S16H mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W (Addgene plasmid #32519) by site directed mutagenesis.
The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).
Proper citation: RRID:Addgene_32520 Copy
Species: Rattus norvegicus
Genetic Insert: μ2 subunit of clathrin adaptor complex AP-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10228163
Comments: NB: The HA tag (YPYDVPDYALE) was inserted within the protein between residues 236 and 237.
Proper citation: RRID:Addgene_32754 Copy
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