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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
w+attB
 
Resource Report
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10+ mentions
RRID:Addgene_30326 phiC31 attB, hsp70::w Drosophila melanogaster Ampicillin w[+] is hsp70 basal promoter; gives full w+ (red eyes), without heat shock. Backbone Size:2881; Vector Backbone:pUC; Vector Types:Drosophila transgenesis; Bacterial Resistance:Ampicillin 2026-08-15 01:13:24 12
gstops
 
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RRID:Addgene_26859 Slow Termination of Phototransduction Drosophila melanogaster Ampicillin PMID:18184564 Backbone Size:7855; Vector Backbone:pCaspeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:57 0
ninaE-stops myc
 
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RRID:Addgene_26856 Slow Termination of Phototransduction Drosophila melanogaster Ampicillin PMID:18184564 To express the stops cDNA under the control of the ninaE promoter, the cDNA (EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end of the wild-type stops cDNA. The wild-type stops cDNAs was subsequently subcloned between the NotI and XbaI sites of pCNX. The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:57 0
ninaE-stops m3-myc
 
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RRID:Addgene_26855 Slow Termination of Phototransduction Drosophila melanogaster Ampicillin PMID:18184564 To express the stops cDNA under the control of the ninaE promoter, the cDNA (EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end of the wild-type stops cDNA. The wild-type stops cDNAs was subsequently subcloned between the NotI and XbaI sites of pCNX. The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Amino acids 296-299 (SLRH in wild-type) are mutated to GPGD 2026-08-15 01:12:57 0
ninaE-dsocs
 
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RRID:Addgene_26857 Slow Termination of Phototransduction Drosophila melanogaster Ampicillin PMID:18184564 To express the stops cDNA under the control of the ninaE promoter, the cDNA (EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites of pCNX. Myc-tagged STOPS lacking the SOCS box was subcloned between the NotI and XbaI sites of pCNX. The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin This STOPS construct contains a mutated/truncated SOCS box (suppressor of cytokine signaling). Amino acids 296 and 297 have been mutated from SL to EF and amino acids 298-330 have been deleted 2026-08-15 01:12:57 0
ninaE-norpA
 
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RRID:Addgene_26854 no receptor potential A Drosophila melanogaster Ampicillin PMID:18184564 To express norpA under control of the ninaE promoter, full length norpA cDNA was excised from pHKX-norpA and subcloned into pCNX Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin V963A 2026-08-15 01:12:57 0
pUAS myc Gr66a
 
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RRID:Addgene_26861 Gr66 gustatory receptor Drosophila melanogaster Ampicillin PMID:16979558 Backbone Size:0; Vector Backbone:pP[UAS-myc]; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:57 0
pcasper4 p [7-Gr66a-]
 
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RRID:Addgene_26860 Gr66 gustatory receptor Drosophila melanogaster Ampicillin PMID:16979558 Backbone Size:7855; Vector Backbone:pCaSper4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Partial deletion of Gr66a (see associated paper listed below for details), but contains genes CG7066 and CG7188, which neighbor Gr66a 2026-08-15 01:12:57 0
pRmHa3-dKDM4A-HA2FL2
 
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RRID:Addgene_20232 dKDM4A Drosophila melanogaster Ampicillin PMID:19061644 Backbone Size:3990; Vector Backbone:pRmHa3-CHA2FL2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:53 0
pRmHa3-dKDM4A-H195A-HA2FL2
 
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RRID:Addgene_20235 dKDM4A Drosophila melanogaster Ampicillin PMID:19061644 D153E mutation is also present, but it does affect the plasmid. Backbone Size:3990; Vector Backbone:pRmHa3-CHA2FL2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin changed His 195 to Ala 2026-08-15 01:11:53 0
pBacPAK-HFT-dKDM4B
 
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RRID:Addgene_20234 dKDM4B Drosophila melanogaster Ampicillin PMID:19061644 TEV tag ENPYFQG instead of ENLYFQG. The cDNA used in making dKDM4B(CG33182) constructs has incomplete 3' end. It is because NCBI data has updated the sequence after I made the construct. There is a comment on NCBI page https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=161077032&from=148&to=1920&view=gbwithparts indicates that they replaced a former sequence of CG33182 https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=28573833 with the current 1773bp one. Backbone Size:5608; Vector Backbone:pBacPAK-HFT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:53 0
pBacPAK-HFT-dKDM4A
 
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RRID:Addgene_20233 dKDM4A Drosophila melanogaster Ampicillin PMID:19061644 TEV tag ENPYFQG instead of ENLYFQG. Backbone Size:5608; Vector Backbone:pBacPAK-HFT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:53 0
pBS-Squ:mCherry
 
Resource Report
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1+ mentions
RRID:Addgene_20163 spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR Drosophila melanogaster Ampicillin PMID:19029882 There are several polymorphisms in the 3’UTR of the expression construct and should not affect the function. Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:52 1
pUAST-Gr64d
 
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RRID:Addgene_21079 Gr64d Drosophila melanogaster Ampicillin PMID:19026541 Backbone Size:11000; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:59 0
pUAST-Gr64a
 
Resource Report
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RRID:Addgene_21081 Gr64a Drosophila melanogaster Ampicillin PMID:17715294 Backbone Size:11000; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:59 0
pMT-Pav-GFP
 
Resource Report
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RRID:Addgene_24286 Pavarotti Drosophila melanogaster Ampicillin PMID:19720876 This plasmid was originally generated by Dr. Gohta Goshima and published in MBOC. Vol. 16, 3896–3907. Backbone Marker:Invitrogen; Backbone Size:4200; Vector Backbone:pMT-GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:29 0
pMT-GFP-p150Dynactin
 
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RRID:Addgene_24283 p150 Dynactin Drosophila melanogaster Ampicillin PMID:17576797 Dr. Goshima made a C-terminal GFP p150 plasmid and Vale lab used his pENTR vector to make an N-terminal fusion using Gateway cloning. Y1238H mutation detected in Addgene's BGH-rev sequence is not likely to affect function. Backbone Marker:Drosophila Gateway Vector Collection; Backbone Size:5943; Vector Backbone:pMT-GW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:29 0
pQUAST-shibire (ts1)
 
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RRID:Addgene_24362 Shibire (ts1) Drosophila melanogaster Ampicillin PMID:20434990 Shibire was PCR amplified from genomic DNA of UAS-shibire transgenic flies (Kitamoto, 2001) using genUASFOR and genUASREVsv40 oligos, and ligated into the NotI/KpnI sites of pQUAST. Backbone Size:9000; Vector Backbone:pQUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:31 0
pQUAST>stop>shibire (ts1)
 
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RRID:Addgene_24363 Shibire (ts1) Drosophila melanogaster Ampicillin PMID:20434990 The NotI fragment containing the mCD8-GFP cassette was excised from pQUAS>stop>mCD8- GFP, the vector was blunted, and ligated to a blunted NotI/KpnI shibirets1 isolated from pQUAST-shibirets1. ts1 Backbone Size:9000; Vector Backbone:pQUAS>stop>mCD8-GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:30 0
pAc5.1B-EGFP-DmHPat
 
Resource Report
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RRID:Addgene_25029 HPat Drosophila melanogaster Ampicillin PMID:20404111 Backbone Marker:Elisa Izaurralde; Backbone Size:6080; Vector Backbone:pAc5.1B-EGFP; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:36 0

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