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Species: Arabidopsis thaliana
Genetic Insert: sgRNA-transRNA transcription by U6 promoter
Vector Backbone Description: Backbone Marker:Lampropoulos et al., 2013; Vector Backbone:pD000; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30083222
Proper citation: RRID:Addgene_104440 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:4900; Vector Backbone:pCR2.1; Vector Types:Expression in Corallochytrium (single-celled eukaryote); Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.11.12.379420v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_104445 Copy
Genetic Insert: enhanced green fluorescent protein
Vector Backbone Description: Backbone Marker:The piggyBac vector backbone was a gift from George Church, Addgene plasmid #63800; Vector Backbone:PiggyBac; Vector Types:Mammalian Expression, PiggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30900179
Proper citation: RRID:Addgene_104454 Copy
Species: Homo sapiens
Genetic Insert: vesicle-associated membrane protein-associated protein B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24105263
Proper citation: RRID:Addgene_104450 Copy
Species: Synthetic
Genetic Insert: 12xMS2V6
Vector Backbone Description: Backbone Marker:Genescript; Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131164
Comments: Plasmid synthesized by Genescript with insert cloned in inverted direction
Proper citation: RRID:Addgene_104390 Copy
Species: Synthetic
Genetic Insert: 24xMS2V6
Vector Backbone Description: Backbone Marker:Genescript; Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131164
Comments: Insert generated by duplication of 12xMBSV6 as a BamHI/BglII fragment inserted at the BglII site
Proper citation: RRID:Addgene_104391 Copy
Species: E. coli
Genetic Insert: rpoA-rpoB-rpoC-6xHis and rpoZ
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25665556
Comments: Plasmid pVS10 encodes the E. coli rpoA-rpoB-rpoC [His6] and
rpoZ ORFs under control of T7 promoter.
Proper citation: RRID:Addgene_104398 Copy
Species: Synthetic
Genetic Insert: mito-Super Ecliptic pHluorin-mRuby2
Vector Backbone Description: Backbone Size:6500; Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29066766
Proper citation: RRID:Addgene_104431 Copy
Species: Homo sapiens
Genetic Insert: TK2
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:Geneart CRISPR nuclease vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28918039
Proper citation: RRID:Addgene_104397 Copy
Species: Homo sapiens
Genetic Insert: gamma-tubulin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29050966
Proper citation: RRID:Addgene_104433 Copy
Species: Synthetic
Genetic Insert: 24xMS2V6
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131164
Comments: Insert cloned from pET259 as BamHI SalI in Addgene plasmid #45162, replacing previous MS2 variant
Proper citation: RRID:Addgene_104393 Copy
Vector Backbone Description: Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30083222
Comments: There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency.
Proper citation: RRID:Addgene_104438 Copy
Species: Homo sapiens
Genetic Insert: HDAC1
Vector Backbone Description: Backbone Marker:Keith Joung (Addgene plasmid # 47754); Backbone Size:9075; Vector Backbone:pMLM3705; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). HDAC1 (a.a. 4-384) was from Addgene plasmid 13820 (HDAC1 Flag)
Proper citation: RRID:Addgene_104409 Copy
Species: Homo sapiens
Genetic Insert: TDP-43
Vector Backbone Description: Vector Backbone:pJ4M; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29438978
Proper citation: RRID:Addgene_104481 Copy
Species: Homo sapiens
Genetic Insert: IFITM3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28835547
Proper citation: RRID:Addgene_104363 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP7b
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31209382
Comments: Please visit https://www.biorxiv.org/content/10.1101/434589v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_104484 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP7f
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31209382
Comments: Please visit https://www.biorxiv.org/content/10.1101/434589v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_104483 Copy
Species: Homo sapiens
Genetic Insert: LSD1
Vector Backbone Description: Backbone Marker:Keith Joung (Addgene plasmid # 47754); Backbone Size:9075; Vector Backbone:pMLM3705; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). LSD1A isoform B catalytic domain (a.a. 171-852) was from LSD1 cDNA clone IRATp970A0364D (Source Bioscience). The P405H change in this cDNA clone was reverted to the consensus during Gibson assembly.
Proper citation: RRID:Addgene_104406 Copy
Species: Homo sapiens
Genetic Insert: G9a
Vector Backbone Description: Backbone Marker:Keith Joung (Addgene plasmid # 47754); Backbone Size:9075; Vector Backbone:pMLM3705; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). G9a contains amino acids 929-1210 of Isoform 1 (identifier: Q96KQ7-1) and was obtained from Addgene plasmid 41721 (MSCVhygro-F-G9a)
Proper citation: RRID:Addgene_104408 Copy
Species: Homo sapiens
Genetic Insert: CD9
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:9351; Vector Backbone:pLenti6.3/TO/V5-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29221804
Comments: Plasmid is used and named CD9-GFP in publication even though it contains mEmerald. Described in the methods of Boker et al 2018 (https://www.ncbi.nlm.nih.gov/pubmed/29221804). Please see publication for more information.
Also see the following article for additional information on using this plasmid: Schiller et al., 2018 https://www.sciencedirect.com/science/article/pii/S2329050118300378
Proper citation: RRID:Addgene_104402 Copy
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