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Species: Arabidopsis thaliana
Genetic Insert: PCO4
Vector Backbone Description: Backbone Marker:Thermo Fisher scientific; Backbone Size:2040; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2025.09.01.673502 for the bioRxiv preprint.
Proper citation: RRID:Addgene_249815 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBQ-HRE-GAL4STE
Vector Backbone Description: Backbone Marker:Thermo Fisher scientific; Backbone Size:2040; Vector Backbone:pDONR201; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2025.09.01.673502 for the bioRxiv preprint.
Proper citation: RRID:Addgene_249817 Copy
Genetic Insert: HaloTag
Vector Backbone Description: Backbone Size:7345; Vector Backbone:pLJM1-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250858 Copy
Species: Homo sapiens
Genetic Insert: RNASEL
Vector Backbone Description: Backbone Size:7345; Vector Backbone:pLJM1-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250856 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.eC VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075114
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_201903 Copy
Species: Homo sapiens
Genetic Insert: BRAF catalytic (CAT) domain (AA 435-766)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36347258
Proper citation: RRID:Addgene_194772 Copy
Species: Mus musculus
Genetic Insert: Mus musculus EZR(1-586):EGFP
Vector Backbone Description: Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36416724
Proper citation: RRID:Addgene_202684 Copy
Species: Discosoma sp.
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Size:5611; Vector Backbone:pAAV-EF1a-DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37828037
Comments: Please visit https://doi.org/10.1101/2022.08.29.505018 for bioRxiv preprint.
Proper citation: RRID:Addgene_202610 Copy
Species: Synthetic
Genetic Insert: monomeric nuclear Tomato
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Our lab uses DH5alpha cells for growth, and recombination is generally not a problem with this lenti backbone. Use NEBstable or similar strains if recombination observed. Please visit https://www.biorxiv.org/content/10.1101/2023.11.21.568191v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172335 Copy
Species: Synthetic
Genetic Insert: monomeric nuclear Tomato
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Our lab uses DH5alpha cells for growth, and recombination is generally not a problem with this lenti backbone. Use NEBstable or similar strains if recombination observed. Please visit https://www.biorxiv.org/content/10.1101/2023.11.21.568191v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172328 Copy
Species: Homo sapiens
Genetic Insert: eEF1A1
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36638793
Proper citation: RRID:Addgene_198383 Copy
Species: Homo sapiens
Genetic Insert: EGFP:PIP5K1A
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37534432
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.30.498262v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_202720 Copy
Species: Synthetic
Genetic Insert: Flag-GFP-TurboID-NES
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37385249
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.07.527548v1 for bioRxiv preprint
Proper citation: RRID:Addgene_202019 Copy
Species: Synthetic
Genetic Insert: APEX2-V5-G3BP1
Vector Backbone Description: Vector Backbone:pTRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37385249
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.07.527548v1 for bioRxiv preprint
Proper citation: RRID:Addgene_202013 Copy
Species: Thermoclostridium caenicola
Genetic Insert: TccCas13a
Vector Backbone Description: Backbone Size:6076; Vector Backbone:Twin-SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35763567
Proper citation: RRID:Addgene_199754 Copy
Species: Homo sapiens
Genetic Insert: Chromosome 2 gRNA
Vector Backbone Description: Vector Backbone:pXPR_023; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576405
Proper citation: RRID:Addgene_202444 Copy
Species: Synthetic
Genetic Insert: HaloTag donor sequence
Vector Backbone Description: Backbone Size:5273; Vector Backbone:PX552; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39146380
Proper citation: RRID:Addgene_187652 Copy
Species: Nicotiana benthamiana
Genetic Insert: NbCysP6 sgRNA
Vector Backbone Description: Backbone Marker:Professor Sophien Kamoun; Backbone Size:6530; Vector Backbone:pICH86966::AtU6p::sgRNA_PDS (Plasmid #46966); Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36061808
Proper citation: RRID:Addgene_223217 Copy
Species: Rattus norvegicus
Genetic Insert: His-DIO GCaMP8f
Vector Backbone Description: Backbone Size:4699; Vector Backbone:pX552; Vector Types:Mammalian Expression, AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38871457
Comments: pX552 was a gift from Feng Zhang (Addgene plasmid # 60958 ; http://n2t.net/addgene:60958 ; RRID:Addgene_60958)
Proper citation: RRID:Addgene_199580 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5383; Vector Backbone:pX552; Vector Types:Mammalian Expression, AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38871457
Comments: pX552 was a gift from Feng Zhang (Addgene plasmid # 60958 ; http://n2t.net/addgene:60958 ; RRID:Addgene_60958)
pAAV-Ef1a-DIO-mCherrry was a gift from Bryan Roth (Addgene 50462).
Proper citation: RRID:Addgene_199581 Copy
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