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http://www.addgene.org/101312

Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382

Proper citation: RRID:Addgene_101312 Copy   

•   Source: Addgene

http://www.addgene.org/101309

Species: Mesoplasma florum
Genetic Insert: oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382

Proper citation: RRID:Addgene_101309 Copy   

•   Source: Addgene

http://www.addgene.org/102270

Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29164072
Comments: Genotype = ΔompA ΔompC ΔompF ΔlamB Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene. Due to the strain's growth defect and potential for contamination, Addgene recommends streaking for single colonies from the stab on low salt LB containing tetracycline and then screening colonies by PCR using the Leo Lab's recommended primers to confirm the strain.

Proper citation: RRID:Addgene_102270 Copy   

•   Source: Addgene

http://www.addgene.org/43831

Vector Backbone Description: Backbone Marker:Addgene plasmid 43829; Backbone Size:5173; Vector Backbone:pRE107 (modified from pGP704); Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Tetracycline
Defining Citation: PMID:9511756
Comments: The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination. Plasmid pRE107 (Addgene plasmid #43829) was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis. To use this plasmid with bla fusions and increase the functionality of this system, the depositing laboratory replaced the ApR gene as follows. The ApR gene flanked by the remaining BamHI sites was removed and replaced with either CmR, TcR or KmR resistance markers (see associated schematic image). The TcR gene was cloned on a blunted EcoRI–StyI fragment from pBR322 into the blunted BamHI sites to give pDMS197. The BamHI site near the MCS was regenerated after cloning; the BamHI site near the 5' end of TcR/TetR was not. The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers. Moreover, the use of Tc also allows an alternative negative selection using fusaric acid and chlortetracycline as described previously (Maloy and Nunn, 1981).

Proper citation: RRID:Addgene_43831 Copy   

•   Source: Addgene

http://www.addgene.org/48095

Vector Backbone Description: Backbone Marker:CJ Marx (Harvard); Backbone Size:6878; Vector Backbone:pCM62; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928

Proper citation: RRID:Addgene_48095 Copy   

•   Source: Addgene

http://www.addgene.org/48096

Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7535; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928

Proper citation: RRID:Addgene_48096 Copy   

•   Source: Addgene

http://www.addgene.org/48099

Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7535; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928

Proper citation: RRID:Addgene_48099 Copy   

•   Source: Addgene

http://www.addgene.org/45863

Species: Enterobacteria phage P1
Genetic Insert: cre recombinase
Vector Backbone Description: Backbone Marker:Lidstrom Lab (Addgene plasmid 45826); Backbone Size:6878; Vector Backbone:pCM62; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12449384
Comments: The 1.1-kb XbaI-EcoRI fragment from pJW168 (PMID 9858684) was cloned between the XbaI and EcoRI sites of pCM62 (Addgene plasmid 45826) to generate the tetracycline-resistance conferring cre expression plasmid. Used with allelic exhange vectors--Addgene plasmids 46012 or 46013--to generate unmarked mutant strains in a broad bacterial host range.

Proper citation: RRID:Addgene_45863 Copy   

•   Source: Addgene

http://www.addgene.org/45828

Vector Backbone Description: Backbone Marker:Lidstrom Lab (Addgene plasmid 45826); Backbone Size:7965; Vector Backbone:pCM62; Vector Types:low-background broad-host-range promoter-probe vector using xylE as a reporter gene; Bacterial Resistance:Tetracycline
Defining Citation: PMID:11495985
Comments: XylE replaces the lac promoter in pCM62 (Addgene plasmid 45826) and is used as a reporter to test promoter activity. A transcriptional terminator was added to reduce background activity.

Proper citation: RRID:Addgene_45828 Copy   

•   Source: Addgene

http://www.addgene.org/45989

Vector Backbone Description: Backbone Size:7395; Vector Backbone:pOSIP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24050148
Comments: Reference: St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j

Proper citation: RRID:Addgene_45989 Copy   

•   Source: Addgene

http://www.addgene.org/46259

Vector Backbone Description: Vector Backbone:original; Vector Types:Bacterial targeting; Bacterial Resistance:Tetracycline
Defining Citation: PMID:10337469

Proper citation: RRID:Addgene_46259 Copy   

•   Source: Addgene

http://www.addgene.org/40861

Species: Xanthomonas oryzae
Genetic Insert: repeat NH10
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_40861 Copy   

•   Source: Addgene

http://www.addgene.org/40857

Species: Xanthomonas oryzae
Genetic Insert: repeat NH6
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_40857 Copy   

•   Source: Addgene

http://www.addgene.org/40855

Species: Xanthomonas oryzae
Genetic Insert: repeat NH4
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_40855 Copy   

•   Source: Addgene

http://www.addgene.org/40853

Species: Xanthomonas oryzae
Genetic Insert: repeat NH2
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_40853 Copy   

•   Source: Addgene

http://www.addgene.org/40859

Species: Xanthomonas oryzae
Genetic Insert: repeat NH8
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_40859 Copy   

•   Source: Addgene

http://www.addgene.org/46974

Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5595; Vector Backbone:pBR322; Vector Types:sequencing vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21410291

Proper citation: RRID:Addgene_46974 Copy   

•   Source: Addgene

http://www.addgene.org/108237

Species: Synthetic
Genetic Insert: PsbA promoter - mTurquoise2
Vector Backbone Description: Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848

Proper citation: RRID:Addgene_108237 Copy   

•   Source: Addgene

http://www.addgene.org/108236

Species: Synthetic
Genetic Insert: PsbA promoter
Vector Backbone Description: Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848

Proper citation: RRID:Addgene_108236 Copy   

•   Source: Addgene

http://www.addgene.org/108234

Species: Synthetic
Genetic Insert: Pc promoter
Vector Backbone Description: Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848

Proper citation: RRID:Addgene_108234 Copy   

•   Source: Addgene