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Vector Backbone Description: Vector Backbone:pHR-CMV-TetO2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30455477
Comments: This empty backbone contains a 12bp dummy insert.
Proper citation: RRID:Addgene_113886 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac2 with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11393 Copy
Vector Backbone Description: Vector Backbone:pHR-CMV-TetO2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30455477
Comments: This empty backbone contains a 12bp dummy insert.
Proper citation: RRID:Addgene_113885 Copy
Species: Homo sapiens
Genetic Insert: cell division cycle 42
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Cdc42 with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11392 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac1(N17) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11395 Copy
Vector Backbone Description: Vector Backbone:pHR-CMV-TetO2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30455477
Comments: This empty backbone contains a 12bp dummy insert.
Proper citation: RRID:Addgene_113889 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac1 with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11391 Copy
Species: Danio rerio
Genetic Insert: he1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2618; Vector Backbone:pDONR P2R-P3; Vector Types:Zebrafish Danio expression; Bacterial Resistance:Kanamycin
Comments: Cloned from the construct provided by Jeff Mumm's Lab, pTol2 5xUAS:YFP-p2A-NTRmutgi:HE:eYFP
Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner."
BMC Biology 2012 10:93
https://doi.org/10.1186/1741-7007-10-93
Proper citation: RRID:Addgene_113879 Copy
Genetic Insert: CMV-eGFP
Vector Backbone Description: Backbone Size:9237; Vector Backbone:pMuLE Lenti Dest Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31860685
Proper citation: RRID:Addgene_113865 Copy
Genetic Insert: MaLionR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29952110
Proper citation: RRID:Addgene_113908 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a YFP fusion to the C-terminus of ARF6. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP variant.
Proper citation: RRID:Addgene_11389 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of constitutively-active ARF6. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11387 Copy
Genetic Insert: ccdB gene, Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_114019 Copy
Species: Homo sapiens
Genetic Insert: cell division cycle 42
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Cdc42(N17) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11400 Copy
Species: Synthetic
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_114005 Copy
Species: Synthetic
Genetic Insert: human codon optimized AsCas12a with C-terminal NLS and His-tag
Vector Backbone Description: Backbone Marker:Schier Lab (Addgene plasmid # 47327); Vector Backbone:pET-28b-Cas9-His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114069 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac2(V12) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11397 Copy
Vector Backbone Description: Vector Backbone:pL1V-Lv1-gent-pBBR1-ELT3; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:30692983
Comments: pL1V-Lv1-gent-pBBR1-ELT3. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113991 Copy
Species: Synthetic
Genetic Insert: Endlinker/terminator Part 6
Vector Backbone Description: Vector Backbone:pLVC-ELT-6; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-ELT-6. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113990 Copy
Species: Synthetic
Genetic Insert: Super-folder GFP
Vector Backbone Description: Vector Backbone:pL0M-SC-sfGFP; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pL0M-SC-sfGFP. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113995 Copy
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