Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Homo sapiens
Genetic Insert: sgRNAs
Vector Backbone Description: Vector Backbone:piggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40972571
Proper citation: RRID:Addgene_251109 Copy
Species: Homo sapiens
Genetic Insert: sgRNAs
Vector Backbone Description: Vector Backbone:piggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40972571
Proper citation: RRID:Addgene_251112 Copy
Species: Homo sapiens
Genetic Insert: sgRNAs
Vector Backbone Description: Vector Backbone:piggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40972571
Proper citation: RRID:Addgene_251110 Copy
Species: Homo sapiens
Genetic Insert: sgRNAs
Vector Backbone Description: Vector Backbone:piggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40972571
Proper citation: RRID:Addgene_251116 Copy
Vector Backbone Description: Backbone Marker:Nigel P Minton; Vector Backbone:pMTLSC-7215; Vector Types:Bacterial knock-in; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41712450
Proper citation: RRID:Addgene_245160 Copy
Species: Homo sapiens
Genetic Insert: PCNA
Vector Backbone Description: Vector Backbone:pcDNA3.1(+)-N-DYK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41693564
Comments: Modification of plasmid "pcDNA3.1(+)-N-DYK_K164R PCNA" (Addgene #221874), with the CDS of ubiquitin ΔGG fused to the C-terminus of PCNA.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221875 Copy
Species: Homo sapiens
Genetic Insert: PCNA
Vector Backbone Description: Vector Backbone:pcDNA3.1(+)-N-DYK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41693564
Comments: Modification of pcDNA3.1(+)-N-DYK_WT PCNA (Addgene #221858) with PCNA mutation K164R.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221874 Copy
Species: Homo sapiens
Genetic Insert: GPR75
Vector Backbone Description: Vector Backbone:pBiT3.1-N [CMV/HiBiT/Blast] Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_238592 Copy
Species: Homo sapiens
Genetic Insert: POLH
Vector Backbone Description: Vector Backbone:pEGFP-C1_N-NLS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41693564
Comments: This plasmid is a variant of pEGFP-C1_N-NLS_WT POLH (Addgene #221868) with the POLH CDS mutated at D652A. This mutation prevents the ubiquitin-binding zinc finger from interacting with ubiquitin and NEDD8.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221871 Copy
Species: Homo sapiens
Genetic Insert: INPP5F
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEFPF-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_238070 Copy
Species: Homo sapiens
Genetic Insert: POLH
Vector Backbone Description: Vector Backbone:pEGFP-C1_N-NLS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41693564
Comments: This plasmid is a variant of pEGFP-C1_N-NLS_WT POLH (Addgene #221868) with the POLH CDS mutated at L704A, F707A and F708A. These mutations occur in the C-terminal PIP (PCNA-interacting peptide) motif of POLH and disrupt binding to PCNA.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221873 Copy
Species: Homo sapiens
Genetic Insert: POLH
Vector Backbone Description: Vector Backbone:pEGFP-C1_N-NLS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41693564
Comments: This plasmid is a variant of pEGFP-C1_N-NLS_WT POLH (Addgene #221868) with the POLH CDS mutated at K682A, K686A, K694A and K709A. These mutations prevent POLH mono-ubiquitination and mono-NEDDylation.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221872 Copy
Species: Synthetic
Genetic Insert: pCMV-TadA-8e (N108Q/L145T)-nSpRYCas9
Vector Backbone Description: Backbone Size:3399; Vector Backbone:pCMV with a BR322 origin (Addgene plasmid #138489, ABE8e); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41720881
Comments: Please visit https://doi.org/10.1101/2025.10.07.680931 for bioRxiv preprint.
Proper citation: RRID:Addgene_242976 Copy
Species: Homo sapiens
Genetic Insert: POLH
Vector Backbone Description: Vector Backbone:pCMV6-AN-DDK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41693564
Comments: This plasmid is a variant of pCMV6-AN-DDK_WT POLH (Addgene #221897) with POLH mutations K682A + K709A.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221864 Copy
Species: Homo sapiens
Genetic Insert: POLH
Vector Backbone Description: Vector Backbone:pCMV6-AN-DDK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41693564
Comments: This plasmid is a variant of pCMV6-AN-DDK_WT POLH (Addgene #221897) with POLH mutations K682A, K686A, K694A + K709A. These mutations prevent POLH mono-ubiquitination and mono-NEDDylation.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221865 Copy
Species: Homo sapiens
Genetic Insert: POLH
Vector Backbone Description: Vector Backbone:pCMV6-AN-DDK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41693564
Comments: This plasmid is a variant of pCMV6-AN-DDK_WT POLH (Addgene #221897) with POLH mutation K682A.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221862 Copy
Species: Homo sapiens
Genetic Insert: POLH
Vector Backbone Description: Vector Backbone:pCMV6-AN-DDK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41693564
Comments: This plasmid is a variant of pCMV6-AN-DDK_WT POLH (Addgene #221897) with ΔGG ubiquitin fused to the C-terminus of POLH.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221861 Copy
Species: Ancestral Passeroidea
Genetic Insert: ATP1A1
Vector Backbone Description: Backbone Marker:Thermo Fisher (Invitrogen); Backbone Size:5235; Vector Backbone:pFastBac Dual; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_196470 Copy
Species: Homo sapiens
Genetic Insert: POLH
Vector Backbone Description: Vector Backbone:pEGFP-C1_N-NLS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41693564
Comments: The CDS for WT POLH was cloned into pEGFP-C1_N-NLS (Addgene #221867).
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221868 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4761; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41693564
Comments: This is a variant of pEGFP-C1 (Clontech) that we modified by addition of an SV40 nuclear localization signal upstream of the EGFP coding sequence.
Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.
Proper citation: RRID:Addgene_221867 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.