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pReadthroughUGA
 
Resource Report
Resource Website 		RRID:Addgene_11513 Firefly luciferase, UAA stop codon, Renilla luciferase, and 3' UTR short RNA binding sites Ampicillin PMID:16483934 The stop codon of the Firefly luciferase ORF from pGL3 was replaced with the stop codon UGA. This construct contains the Firefely ORF fused in frame to Renilla with 18 bp distance between the stop codon and the first ATG within the Renilla ORF. In addition, six CXCR4 short RNA binding sites are present. Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:33 0
AT3G14840 X033_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115063 AT3G14840 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SATLPKEEVDALQSVATALKKSNWNFSVDPCDETLSEGGWRNPNAAKGFEDAVTCNCSSVICHVTNIVLKAQDLQGSLPTDLSGLPFLQELDLTRNYLNGSIPPEWGASSLLNISLLGNRISGSIPKELGNLTTLSGLVLEYNQLSGKIPPELGNLPNLKRLLLSSNNLSGEIPSTFAKLTTLTDLRISDNQFTGAIPDFIQNWKGLEKLVIQASGLVGPIPSAIGLLGTLTDLRITDLSGPESPFPPLRNMTSMKYLILRNCNLTGDLPAYLGQNRKLKNLDLSFNKLSGPIPATYSGLSDVDFIYFTSNMLNGQVPSWMVDQGDTIDITYNNFSKDKTEECQQKSVNTFSSTSPLVANNSSNVSCLSKYTCPKTFYGLHINCGGNEITSNETKYDADTWDTPGYYDSKNGWVSSNTGNFLDDDRTNNGKSKWSNSSELKITNSSIDFRLYTQARLSAISLTYQALCLGKGNYTVNLHFAEIMFNEKNMYSNLGRRYFDIYVQGKREVKDFNIVDEAKGVGKAVVKKFPVMVTNGKLEIRLQWAGKGTQAIPVRGVYGPLISAVSVDPDFIPPKEPGTGTGGGSS Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
AT5G01950 X064_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115065 AT5G01950 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: THPSEVTALRSVKRSLLDPKDYLRNWNRGDPCRSNWTGVICFNEIGTDDYLHVRELLLMNMNLSGTLSPELQKLAHLEILDFMWNNISGSIPNEIGQISSLVLLLLNGNKLSGTLPSELGYLSNLNRFQIDENNITGPIPKSFSNLKKVKHLHFNNNSLTGQIPVELSNLTNIFHVLLDNNKLSGNLPPQLSALPNLQILQLDNNNFSGSDIPASYGNFSNILKLSLRNCSLKGALPDFSKIRHLKYLDLSWNELTGPIPSSNFSKDVTTINLSNNILNGSIPQSFSDLPLLQMLLLKNNMLSGSVPDSLWKNISFPKKARLLLDLRNNSLSRVQGDLTPPQNVTLRLDGNLICTNGSISNANLFCESKGKEWISLPNNSTNSALDCPPLACPTPDFYEYSPASPLRCFCAAPLRIGYRLKSPSFSYFPPYIDQFGEYVTDFLQMEPYQLWIDSYQWEKGPRLRMYLKLFPKVNETYTRTFNESEVLRIRGIFASWRFPGSDLFGPYELLNFTLQGPYSYVNFNS Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
HAESA D03_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115108 AT4G28490 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SLNQDATILRQAKLGLSDPAQSLSSWSDNNDVTPCKWLGVSCDATSNVVSVDLSSFMLVGPFPSILCHLPSLHSLSLYNNSINGSLSADDFDTCHNLISLDLSENLLVGSIPKSLPFNLPNLKFLEISGNNLSDTIPSSFGEFRKLESLNLAGNFLSGTIPASLGNVTTLKELKLAYNLFSPSQIPSQLGNLTELQVLWLAGCNLVGPIPPSLSRLTSLVNLDLTFNQLTGSIPSWITQLKTVEQIELFNNSFSGELPESMGNMTTLKRFDASMNKLTGKIPDNLNLLNLESLNLFENMLEGPLPESITRSKTLSELKLFNNRLTGVLPSQLGANSPLQYVDLSYNRFSGEIPANVCGEGKLEYLILIDNSFSGEISNNLGKCKSLTRVRLSNNKLSGQIPHGFWGLPRLSLLELSDNSFTGSIPKTIIGAKNLSNLRISKNRFSGSIPNEIGSLNGIIEISGAENDFSGEIPESLVKLKQLSRLDLSKNQLSGEIPRELRGWKNLNELNLANNHLSGEIPKEVGILPVLNYLDLSSNQFSGEIPLELQNLKLNVLNLSYNHLSGKIPPLYANKIYAHDFIGNPGLCVDLDGLCRKITRS Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
BAM2 X050_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115104 AT3G49670 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SFTVAKPITELHALLSLKSSFTIDEHSPLLTSWNLSTTFCSWTGVTCDVSLRHVTSLDLSGLNLSGTLSSDVAHLPLLQNLSLAANQISGPIPPQISNLYELRHLNLSNNVFNGSFPDELSSGLVNLRVLDLYNNNLTGDLPVSLTNLTQLRHLHLGGNYFSGKIPATYGTWPVLEYLAVSGNELTGKIPPEIGNLTTLRELYIGYYNAFENGLPPEIGNLSELVRFDAANCGLTGEIPPEIGKLQKLDTLFLQVNAFTGTITQELGLISSLKSMDLSNNMFTGEIPTSFSQLKNLTLLNLFRNKLYGAIPEFIGEMPELEVLQLWENNFTGSIPQKLGENGRLVILDLSSNKLTGTLPPNMCSGNRLMTLITLGNFLFGSIPDSLGKCESLTRIRMGENFLNGSIPKELFGLPKLSQVELQDNYLTGELPISGGGVSGDLGQISLSNNQLSGSLPAAIGNLSGVQKLLLDGNKFSGSIPPEIGRLQQLSKLDFSHNLFSGRIAPEISRCKLLTFVDLSRNELSGDIPNELTGMKILNYLNLSRNHLVGSIPVTIASMQSLTSVDFSYNNLSGLVPSTGQFSYFNYTSFVGNSHLCGPYLGPCGKGTHQSHVKPLS Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
BAM3 X078_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115106 AT4G20270 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SLISPLNLSLIRQANVLISLKQSFDSYDPSLDSWNIPNFNSLCSWTGVSCDNLNQSITRLDLSNLNISGTISPEISRLSPSLVFLDISSNSFSGELPKEIYELSGLEVLNISSNVFEGELETRGFSQMTQLVTLDAYDNSFNGSLPLSLTTLTRLEHLDLGGNYFDGEIPRSYGSFLSLKFLSLSGNDLRGRIPNELANITTLVQLYLGYYNDYRGGIPADFGRLINLVHLDLANCSLKGSIPAELGNLKNLEVLFLQTNELTGSVPRELGNMTSLKTLDLSNNFLEGEIPLELSGLQKLQLFNLFFNRLHGEIPEFVSELPDLQILKLWHNNFTGKIPSKLGSNGNLIEIDLSTNKLTGLIPESLCFGRRLKILILFNNFLFGPLPEDLGQCEPLWRFRLGQNFLTSKLPKGLIYLPNLSLLELQNNFLTGEIPEEEAGNAQFSSLTQINLSNNRLSGPIPGSIRNLRSLQILLLGANRLSGQIPGEIGSLKSLLKIDMSRNNFSGKFPPEFGDCMSLTYLDLSHNQISGQIPVQISQIRILNYLNVSWNSFNQSLPNELGYMKSLTSADFSHNNFSGSVPTSGQFSYFNNTSFLGNPFLCGFSSNPCNGSQNQSQSQLLNQNNARSRGEIS Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
RGFR3 X052_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115107 AT4G26540 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SLDQQGQALLSWKSQLNISGDAFSSWHVADTSPCNWVGVKCNRRGEVSEIQLKGMDLQGSLPVTSLRSLKSLTSLTLSSLNLTGVIPKEIGDFTELELLDLSDNSLSGDIPVEIFRLKKLKTLSLNTNNLEGHIPMEIGNLSGLVELMLFDNKLSGEIPRSIGELKNLQVLRAGGNKNLRGELPWEIGNCENLVMLGLAETSLSGKLPASIGNLKRVQTIAIYTSLLSGPIPDEIGYCTELQNLYLYQNSISGSIPTTIGGLKKLQSLLLWQNNLVGKIPTELGNCPELWLIDFSENLLTGTIPRSFGKLENLQELQLSVNQISGTIPEELTNCTKLTHLEIDNNLITGEIPSLMSNLRSLTMFFAWQNKLTGNIPQSLSQCRELQAIDLSYNSLSGSIPKEIFGLRNLTKLLLLSNDLSGFIPPDIGNCTNLYRLRLNGNRLAGSIPSEIGNLKNLNFVDISENRLVGSIPPAISGCESLEFLDLHTNSLSGSLLGTTLPKSLKFIDFSDNALSSTLPPGIGLLTELTKLNLAKNRLSGEIPREISTCRSLQLLNLGENDFSGEIPDELGQIPSLAISLNLSCNRFVGEIPSRFSDLKNLGVLDVSHNQLTGNLNVLTDLQNLVSLNISYNDFSGDLPNTPFFRRLPLSDLASNRGLYISNAISTRPDPTTRNS Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
AT1G24650 X034_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115070 AT1G24650 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SPDEAVMIALRDSLKLSGNPNWSGSDPCKWSMFIKCDASNRVTAIQIGDRGISGKLPPDLGKLTSLTKFEVMRNRLTGPIPSLAGLKSLVTVYANDNDFTSVPEDFFSGLSSLQHVSLDNNPFDSWVIPPSLENATSLVDFSAVNCNLSGKIPDYLFEGKDFSSLTTLKLSYNSLVCEFPMNFSDSRVQVLMLNGQKGREKLHGSISFLQKMTSLTNVTLQGNSFSGPLPDFSGLVSLKSFNVRENQLSGLVPSSLFELQSLSDVALGNNLLQGPTPNFTAPDIKPDLNGLNSFCLDTPGTSCDPRVNTLLSIVEAFGYPVNFAEKWKGNDPCSGWVGITCTGTDITVINFKNLGLNGTISPRFADFASLRVINLSQNNLNGTIPQELAKLSNLKTLDVSKNRLCGEVPRFNTTIVNTTGNFEDCPNGNAGKKASS Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
TMK1 X035_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115071 AT1G66150 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SDGDLSAMLSLKKSLNPPSSFGWSDPDPCKWTHIVCTGTKRVTRIQIGHSGLQGTLSPDLRNLSELERLELQWNNISGPVPSLSGLASLQVLMLSNNNFDSIPSDVFQGLTSLQSVEIDNNPFKSWEIPESLRNASALQNFSANSANVSGSLPGFLGPDEFPGLSILHLAFNNLEGELPMSLAGSQVQSLWLNGQKLTGDITVLQNMTGLKEVWLHSNKFSGPLPDFSGLKELESLSLRDNSFTGPVPASLLSLESLKVVNLTNNHLQGPVPVFKSSVSVDLDKDSNSFCLSSPGECDPRVKSLLLIASSFDYPPRLAESWKGNDPCTNWIGIACSNGNITVISLEKMELTGTISPEFGAIKSLQRIILGINNLTGMIPQELTTLPNLKTLDVSSNKLFGKVPGFRSNVVVNTNGNPDIGKDKSSLSSPGSSSPSGGSGSGINGDKDRRGMKS Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
pET32a-HD46Q
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_11515 huntingtin, exon 1, 46Q glutamines Homo sapiens Ampicillin PMID:12193654 Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin huntingtin exon 1 with 46 glutamines, vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together. Addgene 2026-10-24 02:03:33 4
AT1G56120 X070_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115060 AT1G56120 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SIFAAWKIRAPREWNISGELCSGVAIDASVLDSNHAYNPLIKCDCSFQNSTICRINNIKVYAIDVVGPIPPELWTLTYLTNLNLGQNYLTGSLSPAIGNLTRMQWMTFGINALSGPIPKEIGLLTDLRLLGISSNNFSGSLPAEIGSCTKLQQMYIDSSGLSGGIPLSFANFVELEVAWIMDVELTGRIPDFIGFWTKLTTLRILGTGLSGPIPSSFSNLIALTELRLGDISNGSSSLDFIKDMKSLSVLVLRNNNLTGTIPSTIGGYTSLQQVDLSFNKLHGPIPASLFNLSRLTHLFLGNNTLNGSLPTLKGQSLSNLDVSYNDLSGSLPSWVSLPDLKLNLVANNFTLEGLDNRVLSGLHCLQKNFPCNRGEGIYYNFSINCGGPEIRSVSGALFEKEDADLGPASFVVSAAKRWAASSVGNFAGSSNNIYIATSLAQFINTMDSELFQSARLSASSLRYYGLGLENGGYTVTLQFAEVQIEGSNSWKGIGRRRFNIYVQGRLVEKDFDIRRTAGGSSVRAVQREYKTNVSENHLEVHLFWAGKGTCCIPIQGAYGPLIAAVSATPDFTPTVANRPPSKGKS Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
AT1G56130 X071_pECIA2
 
Resource Report
Resource Website 		RRID:Addgene_115061 AT1G56130 Arabidopsis thaliana Ampicillin PMID:29320478 Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: QTGATTHPDEARALNSIFAAWKIQAPREWNISGELCSGAAIDASVLDSNPAYNPLIKCDCSFQNSTICRITNIKVYAIDVVGPIPPELWTLTYLTNLNLGQNVLTGSLPPAIGNLTRMQWMTFGINALSGPVPKEIGLLTDLRLLGISSNNFSGSIPDEIGRCTKLQQMYIDSSGLSGRIPLSFANLVQLEQAWIADLEVTDQIPDFIGDWTKLTTLRIIGTGLSGPIPSSFSNLTSLTELRLGDISSGSSSLDFIKDMKSLSVLVLRNNNLTGTIPSTIGEHSSLRQVDLSFNKLHGPIPASLFNLSQLTHLFLGNNTLNGSFPTQKTQSLRNVDVSYNDLSGSLPSWVSLPSLKLNLVANNFTLEGLDNRVLPGLNCLQKNFPCNRGKGIYSDFSINCGGPEKRSVTGALFEREDEDFGPASFFVSAGQRWAASSVGLFAGSSNNIYIATSQSQFVNTLDSELFQSARLSASSVRYYGLGLENGGYTVTLQFAEIQILGSTSTTWKGLGRRRFDIYVQGRLVEKDFDVRRTAGDSTVRAVQRVYKANVSENHLEVHLFWAGKGTCCIPIQGAYGPLISAVSATPDFTPTVANKPPSKGKNRT Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:32 0
pHD-mW-attPCC-FRT (pTL717)
 
Resource Report
Resource Website 		RRID:Addgene_115158 mW attP-CC FRT Synthetic Ampicillin PMID:30065043 Based on pHD-DsRed-attP vector (Gratz et al., Genetics, 2014; DOI: 10.1534/genetics.113.160713) Backbone Size:2796; Vector Backbone:pHD-DsRed-attP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:33 0
pET32a-HD25Q
 
Resource Report
Resource Website 		RRID:Addgene_11508 huntingtin, exon 1, 25 glutamines Homo sapiens Ampicillin PMID:12193654 Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin huntingtin exon 1 with 25 glutamines, vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together. Addgene 2026-10-24 02:03:32 0
pSH231-EF1-GFP-HYGRO
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_115144 GFP Synthetic Ampicillin PMID:30793977 Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint. Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:33 1
pSH231-EF1-RFP-HYGRO
 
Resource Report
Resource Website 		RRID:Addgene_115145 RFP Synthetic Ampicillin PMID:30793977 Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint. Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:33 0
pSH231-EFS-Cas9-BlastR
 
Resource Report
Resource Website 		RRID:Addgene_115146 Cas9-T2A-BlastR Streptococcus pyogenes Ampicillin PMID:30793977 Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint. Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:33 0
pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)
 
Resource Report
Resource Website 		RRID:Addgene_115141 human codon optimized Orf2mor anti-CRISPR protein Synthetic Ampicillin PMID:30190308 Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:33 0
pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_115142 human codon optimized Mb3Cas12a Synthetic Ampicillin PMID:30190308 Backbone Marker:Cepko lab (Addgene Plasmid #11179); Vector Backbone:pCAG (Addgene #11179); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:33 2
pSH231-EF1-BLST-dCas9-VPR
 
Resource Report
Resource Website 		RRID:Addgene_115148 BlastR-P2A-dCas9-VPR Synthetic Ampicillin PMID:30793977 Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint. Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:03:33 0

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