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  • Organism:severe acute respiratory syndrome coronavirus 2 (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pTwist-SARS-CoV-2 Spike-deltaC18 KP.2
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_223273 SARS-CoV-2 Spike KP.2 Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Relevant Mutations: Ins16MPLF, T19I, R21T, deltaL24P25P26,A27S,S50L,deltaH69V70,V127F,G142D,deltaY144,F157S,R158G,deltaN211,L212I,V213G,L216F,H245N,A246D,I332V,G339H,R346T,K356T,S371F,S373P,S375F,T376A,R403K,D405N,R408S,K417N,N440K,V445H,G446S,N450D,L452W,L455S,F456L,N460K,S477NT478K,N481K,V483E,deltaE484,F486P,Q498R,N501Y,Y505H,E554K,A570V,D614G,P621S,H655Y,N679K,P681R,N764K,D796Y,S939F,Q954H,N969K,V1104LP1143L Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin See Depositor Comments. 2026-08-15 01:31:24 1
pTwist-SARS-CoV-2 Spike-deltaC18 L981F (TTC)
 
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RRID:Addgene_212486 SARS-CoV-2 Spike L981F Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin L981F 2026-08-15 01:31:55 0
pTwist-SARS-CoV-2 Spike-deltaC18 (Yellowhammer)
 
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RRID:Addgene_212402 SARS-CoV-2 Spike L5F,D614G,Q677H Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin L5F,D614G,Q677H 2026-08-15 01:31:55 0
pTwist-SARS-CoV-2 Spike-deltaC18 deltaE156-F157+R158G (GGA)
 
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RRID:Addgene_212421 SARS-CoV-2 Spike E156-F157deletion,R158G Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E156-F157deletion,R158G 2026-08-15 01:31:55 0
pAIP4_6WEY
 
Resource Report
Resource Website
RRID:Addgene_234234 6WEY Severe acute respiratory syndrome coronavirus 2 Kanamycin PMID:42555168 5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed). Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Cleaved into Host translation inhibitor nsp1 (Leader protein) (Non-structural protein 1) (nsp1); Non-structural protein 2 (nsp2) (p65 homolog); Non-structural protein 3 (nsp3) (EC 3.4.19.12) (EC 3.4.22.-) (PL2-PRO) (Papain-like protease) (Papain-like proteinase) (PL-PRO); Non-structural protein 4 (nsp4); 3C-like proteinase (3CL-PRO) (3CLp) (EC 3.4.22.69) (Main protease) (Mpro) (Non-structural protein 5) (nsp5) (SARS coronavirus main proteinase); Non-structural protein 6 (nsp6); Non-structural protein 7 (nsp7); Non-structural protein 8 (nsp8); Non-structural protein 9 (nsp9); Non-structural protein 10 (nsp10) (Growth factor-like peptide) (GFL); RNA-directed RNA polymerase (Pol) (RdRp) (EC 2.7.7.48) (Non-structural protein 12) (nsp12); Helicase (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Non-structural protein 13) (nsp13); Proofreading exoribonuclease (ExoN) (EC 3.1.13.-) (Guanine-N7 methyltransferase) (Non-structural protein 14) (nsp14); Uridylate-specific endoribonuclease (EC 3.1.-.-) (NendoU) (Non-structural protein 15) (nsp15); 2'-O-methyltransferase (EC 2.1.1.-) (Non-structural protein 16) (nsp16)] Backbone Size:5679; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:32:05 0
pHis-TEV-Nsp15-E266Q
 
Resource Report
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RRID:Addgene_218803 Nsp15-E266Q Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:40411374 Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-E266Q (amino acid residues 6453-6798 of YP_009724389, with E266Q mutation). Confers ampicillin-resistance. Backbone vector is pET-11b from GenScript. Mutation design by Nirupa Nagaratnam. Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E266Q (G to C) 2026-08-15 01:32:57 0
pHis-TEV-Nsp15-D183N
 
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RRID:Addgene_219526 Nsp15-D183N Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:40411374 Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-D183N (amino acid residues 6453-6798 of YP_009724389, with D183N mutation). Confers ampicillin-resistance. Backbone vector is pET-11b from GenScript. Mutation design by Nirupa Nagaratnam. Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin D183N (G to A) 2026-08-15 01:32:57 0
pHis-TEV-Nsp15-A81V
 
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RRID:Addgene_219525 Nsp15-A81V Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:40411374 Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-A81V (amino acid residues 6453-6798 of YP_009724389, with A81V mutation). Confers ampicillin-resistance. Backbone vector is pET-11b from GenScript. Mutation design by Nirupa Nagaratnam. Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin A81V (C to T) 2026-08-15 01:32:57 0
pHis-TEV-Nsp15-V66L
 
Resource Report
Resource Website
RRID:Addgene_219524 Nsp15-V66L Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:40411374 Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-V66L (amino acid residues 6453-6798 of YP_009724389, with V66L mutation). Confers ampicillin-resistance. Backbone vector is pET-11b from GenScript. Mutation design by Nirupa Nagaratnam. Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin V66L (G to C) 2026-08-15 01:32:57 0
pαH-S-GSAS-OMICRON.BA.2
 
Resource Report
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1+ mentions
RRID:Addgene_184829 S-GSAS-OMICRON.BA.2 Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:35732171 Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint. Backbone Size:4745; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS; T19I, del 24-26, A27S, G142D,V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K,P681H, N764K, D796Y, Q954H, N969K 2026-08-15 01:23:06 2
pαH-S-GSAS-OMICRON.BA.2-RBD
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_184830 S-GSAS-OMICRON.BA2-RBD Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:35732171 Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint. Backbone Size:4681; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 variant; G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H 2026-08-15 01:23:06 1
pTwist-SARS-CoV-2 Spike-deltaC18 A570D (GAC)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_212339 SARS-CoV-2 Spike A570D Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin A570D 2026-08-15 01:30:34 1
pTwist-SARS-CoV-2 Spike-deltaC18 N501Y (TAT)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_212338 SARS-CoV-2 Spike N501Y Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin N501Y 2026-08-15 01:30:34 1
pTwist-SARS-CoV-2 Spike-deltaC18 deltaY145
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_212337 SARS-CoV-2 Spike Y145H Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Y145H 2026-08-15 01:30:34 1
pTwist-SARS-CoV-2 Spike-deltaC18 deltaH69V70
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_212336 SARS-CoV-2 Spike deltaH69, deltaH70 Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin deltaH69, deltaH70 2026-08-15 01:30:34 1
pTwist-SARS-CoV-2 Spike-deltaC18 D936Y (TAT)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_212334 SARS-CoV-2 Spike D936Y Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin D936Y 2026-08-15 01:30:34 1
pTwist-SARS-CoV-2 Spike-deltaC18 D80A (GCT)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_212344 SARS-CoV-2 Spike D80A Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin D80A 2026-08-15 01:30:34 1
pTwist-SARS-CoV-2 Spike-deltaC18 E484K (AAA) N501Y (TAT)
 
Resource Report
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1+ mentions
RRID:Addgene_212354 SARS-CoV-2 Spike E484K, N501Y Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E484K, N501Y 2026-08-15 01:30:34 1
pTwist-SARS-CoV-2 Spike-deltaC18 SAK005321 (Wrong L242H)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_212350 SARS-CoV-2 Spike D80A, D215G, L242H, K417N, E484K, N501Y, D614G, A701V Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin D80A, D215G, L242H, K417N, E484K, N501Y, D614G, A701V 2026-08-15 01:30:34 1
pTwist-SARS-CoV-2 Spike-deltaC18 T20N (AAT)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_212359 SARS-CoV-2 Spike T20N Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:38496628 Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint. Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin T20N 2026-08-15 01:30:34 1

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