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Species: Synthetic
Genetic Insert: stop cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to generate a premature stop codon at your locus of interest. Also contains a multiple cloning site for easy genotyping. This plasmid contains multiple M13F and M13R sites, due to cloning the MCS of pBluescriptSK+.
QC note: This plasmid dimerizes during growth in bacteria, which can result in digest and Sanger sequencing results that differ from the full plasmid sequence of the monomer. The depositing lab has confirmed that dimerization does not affect plasmid function.
Proper citation: RRID:Addgene_113745 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has zeocinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113744 Copy
Species: Other
Genetic Insert: mEGFP-mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mEGFP-mEGFP-mEGFP at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113748 Copy
Species: Staphylococcus aureus
Genetic Insert: SaCas9
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:AAV; Vector Types:AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113691 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113694 Copy
Species: Other
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221-P1P2; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113735 Copy
Species: Other
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2604; Vector Backbone:pDONR221-P1P5r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113737 Copy
Species: Other
Genetic Insert: mTagBFP2-T2A-iCre
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that iCre in this plasmid has an extra Valine upstream of the SV40 nuclear localization signal that does not affect the function. We have also deposited a version of this plasmid without the extra Valine, plasmid #125822.
Proper citation: RRID:Addgene_113837 Copy
Species: P. putida
Genetic Insert: β subunit of Protocatechuate 3,4- Dioxygenase
Vector Backbone Description: Backbone Marker:DNASU plasmid repository, Arizona State University Biodesign Institute; Backbone Size:4010; Vector Backbone:pVP91A vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25548185
Comments: Please ensure that the chosen expression cell line expresses the T5 RNA polymerase.
Senavirathne, G., et al., Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule fluorescence imaging. Anal Biochem, 2018. 556: p. 78-84.
Proper citation: RRID:Addgene_113766 Copy
Species: Homo sapiens
Genetic Insert: miR-30
Vector Backbone Description: Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657504
Proper citation: RRID:Addgene_11379 Copy
Genetic Insert: CMV-eGFP
Vector Backbone Description: Backbone Size:9237; Vector Backbone:pMuLE Lenti Dest Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31860685
Proper citation: RRID:Addgene_113862 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of constitutively-inactive ARF6. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11386 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of constitutively-active ARF1. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11385 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of ARF6. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11382 Copy
Species: Synthetic
Genetic Insert: jREX-GECO1
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:4064; Vector Backbone:pTorPE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31054773
Comments: Please visit https://www.biorxiv.org/content/early/2018/10/04/435891 for bioRxiv preprint.
Proper citation: RRID:Addgene_113941 Copy
Species: Other
Genetic Insert: monomeric Venus
Vector Backbone Description: Vector Backbone:pHR-CMV-TetO2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30455477
Proper citation: RRID:Addgene_113893 Copy
Species: Other
Genetic Insert: E. coli biotin ligase
Vector Backbone Description: Vector Backbone:pHR-CMV-TetO2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30455477
Proper citation: RRID:Addgene_113897 Copy
Species: Synthetic
Genetic Insert: LOV2 and Stim1 polybasic domain (PB)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28959426
Proper citation: RRID:Addgene_113934 Copy
Species: Other
Genetic Insert: TetR-HA-NLS-P2A-BSD-Myc
Vector Backbone Description: Vector Backbone:pHR-CMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30455477
Proper citation: RRID:Addgene_113899 Copy
Vector Backbone Description: Vector Backbone:pHR-CMV-TetO2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30455477
Comments: This empty backbone contains a 12bp dummy insert.
Proper citation: RRID:Addgene_113898 Copy
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