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Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 3 positions (Q93E, Q198K, G422S and S409F). This plasmid has an additional Q271A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) on the pEN_TT 3XFLAG-MAVS plasmid
Proper citation: RRID:Addgene_158631 Copy
Species: Homo sapiens
Genetic Insert: Tau microtubule-binding domain only (amino acids 242-367)
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_159719 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated protein tau
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_159717 Copy
Species:
Genetic Insert: gentamycin-3-acetyltransferase
Vector Backbone Description: Vector Backbone:pTJV1Sc-rpoB; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: Same as pTJV1Sc-rpoB, but spectinomycin resistance marker changed to gentamycin resistance
Proper citation: RRID:Addgene_166979 Copy
Species: Photorhabdus luminescens
Genetic Insert: luxCDABE
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Vector Backbone:pBBRMCS-5; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167135 Copy
Species: Photorhabdus luminescens
Genetic Insert: luxCDABE
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Vector Backbone:pBBRMCS-5; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167134 Copy
Species: Photorhabdus luminescens
Genetic Insert: luxCDABE
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Vector Backbone:pBBRMCS-5; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167130 Copy
Species: Synthetic
Genetic Insert: RhaS-RhaR-mRFP
Vector Backbone Description: Vector Backbone:pFRR5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167506 Copy
Species: Synthetic
Genetic Insert: TetR-RFP
Vector Backbone Description: Vector Backbone:pFLtR5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167505 Copy
Species: Synthetic
Genetic Insert: VanRAM-mKelly2
Vector Backbone Description: Vector Backbone:pFVK5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167522 Copy
Species: Synthetic
Genetic Insert: VanRAM-tdKatushka2
Vector Backbone Description: Vector Backbone:pFVTd5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167520 Copy
Species: Synthetic
Genetic Insert: VanRAM-mRFP
Vector Backbone Description: Vector Backbone:pBVR5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167511 Copy
Species: Aspergillus nidulans
Genetic Insert: Rhamnogalacturonan lyase A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Donor vector / entry clone; Bacterial Resistance:Gentamicin
References:
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_170720 Copy
Species: Synthetic
Genetic Insert: I-SceI_sacB
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:4768; Vector Backbone:pBBR1-MCS5; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Alternative names: pBBR1-GmR_I-SceI_sacB
Proper citation: RRID:Addgene_171155 Copy
Species: Synthetic
Genetic Insert: J306 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:4768; Vector Backbone:pBBR1-MCS5; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Alternative names: pCK120.306, pBBR1-GmR_J3-BBa_J23117-mRFP_J306
Proper citation: RRID:Addgene_171148 Copy
Species: Synthetic
Genetic Insert: hAAVS1 scRNA
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:4568; Vector Backbone:pRK2-GmR; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Alternative names: pCK079, pRK2-GmR_J1-BBa_J23117-mRFP_hAAVS1
Proper citation: RRID:Addgene_171146 Copy
Species: Synthetic
Genetic Insert: J109 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:4768; Vector Backbone:pBBR1-MCS5; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Alternative names: pCK057, pBBR1-GmR_J1-mRFP_J109
Proper citation: RRID:Addgene_171144 Copy
Species: Synthetic
Genetic Insert: J106 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:4768; Vector Backbone:pBBR1-MCS5; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Alternative names: pCDP023.6, pBBR1-GmR_J106
Proper citation: RRID:Addgene_171141 Copy
Species: P. putida
Genetic Insert: XylS-Pm
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:10153; Vector Backbone:pPPC001; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Alternative names: pCK193, pUC18TminiTn7T-Gm_XylS-Pm-dCas9_BBa_J23107-MCP-SoxS(R93A/S101A)
Used for conjugation, with pTNS1 and pRK2013 helper plasmids, to integrate XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) cassettes into gram-negative bacteria.
Point mutation in the FRT site was observed and remained applicable with counterselection by pCK255
Proper citation: RRID:Addgene_171140 Copy
Species: S. pyogenes (dCas9), MS2 (MCP), E. coli (SoxS)
Genetic Insert: Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Backbone Size:4569; Vector Backbone:pUC18TminiTn7T-Gm; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Alternative names: pCDP041, pUC18TminiTn7T-Gm_Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A/S101A)
Used for conjugation, with pTNS1 and pRK2013 helper plasmids, to integrate Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) cassettes into gram-negative bacteria.
Point mutation in the FRT site was observed and remained applicable with counterselection by pCK255
Proper citation: RRID:Addgene_171138 Copy
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