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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike KP.2
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Relevant Mutations: Ins16MPLF, T19I, R21T, deltaL24P25P26,A27S,S50L,deltaH69V70,V127F,G142D,deltaY144,F157S,R158G,deltaN211,L212I,V213G,L216F,H245N,A246D,I332V,G339H,R346T,K356T,S371F,S373P,S375F,T376A,R403K,D405N,R408S,K417N,N440K,V445H,G446S,N450D,L452W,L455S,F456L,N460K,S477NT478K,N481K,V483E,deltaE484,F486P,Q498R,N501Y,Y505H,E554K,A570V,D614G,P621S,H655Y,N679K,P681R,N764K,D796Y,S939F,Q954H,N969K,V1104LP1143L
Proper citation: RRID:Addgene_223273 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike L981F
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212486 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike L5F,D614G,Q677H
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212402 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike E156-F157deletion,R158G
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212421 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: 6WEY
Vector Backbone Description: Backbone Size:5679; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42555168
Comments: 5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed). Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Cleaved into Host translation inhibitor nsp1 (Leader protein) (Non-structural protein 1) (nsp1); Non-structural protein 2 (nsp2) (p65 homolog); Non-structural protein 3 (nsp3) (EC 3.4.19.12) (EC 3.4.22.-) (PL2-PRO) (Papain-like protease) (Papain-like proteinase) (PL-PRO); Non-structural protein 4 (nsp4); 3C-like proteinase (3CL-PRO) (3CLp) (EC 3.4.22.69) (Main protease) (Mpro) (Non-structural protein 5) (nsp5) (SARS coronavirus main proteinase); Non-structural protein 6 (nsp6); Non-structural protein 7 (nsp7); Non-structural protein 8 (nsp8); Non-structural protein 9 (nsp9); Non-structural protein 10 (nsp10) (Growth factor-like peptide) (GFL); RNA-directed RNA polymerase (Pol) (RdRp) (EC 2.7.7.48) (Non-structural protein 12) (nsp12); Helicase (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Non-structural protein 13) (nsp13); Proofreading exoribonuclease (ExoN) (EC 3.1.13.-) (Guanine-N7 methyltransferase) (Non-structural protein 14) (nsp14); Uridylate-specific endoribonuclease (EC 3.1.-.-) (NendoU) (Non-structural protein 15) (nsp15); 2'-O-methyltransferase (EC 2.1.1.-) (Non-structural protein 16) (nsp16)]
Proper citation: RRID:Addgene_234234 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-E266Q
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40411374
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-E266Q (amino acid residues 6453-6798 of YP_009724389, with E266Q mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_218803 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-D183N
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40411374
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-D183N (amino acid residues 6453-6798 of YP_009724389, with D183N mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219526 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-A81V
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40411374
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-A81V (amino acid residues 6453-6798 of YP_009724389, with A81V mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219525 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-V66L
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40411374
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-V66L (amino acid residues 6453-6798 of YP_009724389, with V66L mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219524 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-OMICRON.BA.2
Vector Backbone Description: Backbone Size:4745; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35732171
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_184829 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-OMICRON.BA2-RBD
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35732171
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_184830 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike A570D
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212339 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike N501Y
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212338 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike Y145H
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212337 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike deltaH69, deltaH70
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212336 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike D936Y
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212334 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike D80A
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212344 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike E484K, N501Y
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212354 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike D80A, D215G, L242H, K417N, E484K, N501Y, D614G, A701V
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212350 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike T20N
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212359 Copy
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