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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-A81V
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40411374
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-A81V (amino acid residues 6453-6798 of YP_009724389, with A81V mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219525 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-V66L
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40411374
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-V66L (amino acid residues 6453-6798 of YP_009724389, with V66L mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219524 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp13_SARS2
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith, Addgene Plasmid #62304; Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234374 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp12_SARS2
Vector Backbone Description: Vector Backbone:pFBD-BirA; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234377 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp3_SARS2
Vector Backbone Description: Vector Backbone:pNIC-Bio3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_234388 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: CoV2-Omicron BA.1 spike
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35305699
Proper citation: RRID:Addgene_194602 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: CoV2-RBD-gamma variant
Vector Backbone Description: Vector Backbone:pcDNA216; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_194600 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: CoV2-RBD-delta variant
Vector Backbone Description: Vector Backbone:pcDNA216; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_194601 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: CoV2-RBD-alpha variant
Vector Backbone Description: Vector Backbone:pcDNA216; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_194599 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike BA.2.86
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Relevant mutations: insertion of MPLF after V16 , T19I , R21T , deltaL24P25P26 , A27S , S50L , del H69V70 , V127F , G142D , del Y145 , F157S , R158G , N211I , del L212 , V213G , L216F , H245N , A264D , I332V , G339H , K356T , S371F , S373P , S375F , T376A , R403K , D405N , R405S , K417N , N440K , V445H , G446S , N450D , L452W , N460K , S477N , T478K , N481K , V483K , del E484 , F486P , Q498R N501Y Y505H E554K A570V D614G P621S H655Y N679K P681R N764K D796Y S939F Q954H N969K P1143L Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212538 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike G446S
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212558 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike G75V, T76I
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212443 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike G446S
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212468 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike A67V
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212395 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike L981F
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212486 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike L5F,D614G,Q677H
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212402 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike E156-F157deletion,R158G
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212421 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike KP.2
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Relevant Mutations: Ins16MPLF, T19I, R21T, deltaL24P25P26,A27S,S50L,deltaH69V70,V127F,G142D,deltaY144,F157S,R158G,deltaN211,L212I,V213G,L216F,H245N,A246D,I332V,G339H,R346T,K356T,S371F,S373P,S375F,T376A,R403K,D405N,R408S,K417N,N440K,V445H,G446S,N450D,L452W,L455S,F456L,N460K,S477NT478K,N481K,V483E,deltaE484,F486P,Q498R,N501Y,Y505H,E554K,A570V,D614G,P621S,H655Y,N679K,P681R,N764K,D796Y,S939F,Q954H,N969K,V1104LP1143L
Proper citation: RRID:Addgene_223273 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: 6WEY
Vector Backbone Description: Backbone Size:5679; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42555168
Comments: 5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed). Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Cleaved into Host translation inhibitor nsp1 (Leader protein) (Non-structural protein 1) (nsp1); Non-structural protein 2 (nsp2) (p65 homolog); Non-structural protein 3 (nsp3) (EC 3.4.19.12) (EC 3.4.22.-) (PL2-PRO) (Papain-like protease) (Papain-like proteinase) (PL-PRO); Non-structural protein 4 (nsp4); 3C-like proteinase (3CL-PRO) (3CLp) (EC 3.4.22.69) (Main protease) (Mpro) (Non-structural protein 5) (nsp5) (SARS coronavirus main proteinase); Non-structural protein 6 (nsp6); Non-structural protein 7 (nsp7); Non-structural protein 8 (nsp8); Non-structural protein 9 (nsp9); Non-structural protein 10 (nsp10) (Growth factor-like peptide) (GFL); RNA-directed RNA polymerase (Pol) (RdRp) (EC 2.7.7.48) (Non-structural protein 12) (nsp12); Helicase (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Non-structural protein 13) (nsp13); Proofreading exoribonuclease (ExoN) (EC 3.1.13.-) (Guanine-N7 methyltransferase) (Non-structural protein 14) (nsp14); Uridylate-specific endoribonuclease (EC 3.1.-.-) (NendoU) (Non-structural protein 15) (nsp15); 2'-O-methyltransferase (EC 2.1.1.-) (Non-structural protein 16) (nsp16)]
Proper citation: RRID:Addgene_234234 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike Y453F
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38496628
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212369 Copy
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