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Species: Other
Genetic Insert: wtTadA-TadA7.10-nCas9-2*NLS
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29807545
Proper citation: RRID:Addgene_115627 Copy
Species: Other
Genetic Insert: wtTadA-TadA7.10-nCas9-3*NLS
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29807545
Proper citation: RRID:Addgene_115628 Copy
Species: Other
Genetic Insert: wtTadA-TadA7.10-nCas9-3*NLS
Vector Backbone Description: Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29807545
Proper citation: RRID:Addgene_115621 Copy
Species: Other
Genetic Insert: NLS-wtTadA-TadA7.10-nCas9-NLS
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29807545
Proper citation: RRID:Addgene_115622 Copy
Species: Other
Genetic Insert: wtTadA-TadA7.10-NLS-nCas9-NLS
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29807545
Proper citation: RRID:Addgene_115624 Copy
Species: Neisseria meningitidis, strain 8013
Genetic Insert: NmeCas9
Vector Backbone Description: Backbone Size:5693; Vector Backbone:pSimpleII; Vector Types:Mammalian Expression, Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30518407
Proper citation: RRID:Addgene_115694 Copy
Species: Synthetic
Genetic Insert: SA-mOrange2-sfGFP-SD
Vector Backbone Description: Vector Backbone:pSTAR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30146318
Proper citation: RRID:Addgene_115693 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28276439
Proper citation: RRID:Addgene_115617 Copy
Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30033369
Comments: Note: Plasmid contains rrnB T1 terminator and T7Te terminator sequences not present in the Genbank sequence.
Proper citation: RRID:Addgene_115613 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28276439
Proper citation: RRID:Addgene_115618 Copy
Species: Synthetic
Genetic Insert: mCherry, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Comments: Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV
Proper citation: RRID:Addgene_115683 Copy
Species: Synthetic
Genetic Insert: Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28456689
Proper citation: RRID:Addgene_115684 Copy
Vector Backbone Description: Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15240889
Comments: pSico allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.
The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on http://web.mit.edu/jacks-lab/ (the Jacks Lab website)
Proper citation: RRID:Addgene_11578 Copy
Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10759496
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article.
Kanamycin gene for plant selection on MS media.
Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l).
The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.
Proper citation: RRID:Addgene_11572 Copy
Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033369
Proper citation: RRID:Addgene_115605 Copy
Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033369
Proper citation: RRID:Addgene_115606 Copy
Species: Cauliflower mosaic virus
Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hayashi et al, 1992; Backbone Size:10138; Vector Backbone:pPCVICEn4HPT; Vector Types:Plant Expression, Activation tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10759496
Comments: The CaMV 35S enhancers in these vectors correspond to nucleotides -417 to -86 relative to the transcription start.
Recommended Agrobacterium strain is GV3101 pMP90RK. Host and helper plasmid markers are kanamycin, gentamycin and rifampicin resistance.
Plasmid selection in E. coli and in A. tumefaciens is ampicillin resistance.
Sites that leave the pBstKS+ sequences intact and cut only on one side between pBstKS+ and either left or right border can be used for plasmid rescue. Note that there are additional BssS1 sites not shown on the map.
The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.
Proper citation: RRID:Addgene_11570 Copy
Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30033369
Proper citation: RRID:Addgene_115601 Copy
Species: Synthetic
Genetic Insert: OCT4-Halo-IRES-Bsd
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31556488
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/18/299073 for BioRxiv preprint
Proper citation: RRID:Addgene_115689 Copy
Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033369
Proper citation: RRID:Addgene_115608 Copy
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