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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pNH6
 
Resource Report
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RRID:Addgene_40857 repeat NH6 Xanthomonas oryzae Tetracycline PMID:21493687 Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:39 0
pNH4
 
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RRID:Addgene_40855 repeat NH4 Xanthomonas oryzae Tetracycline PMID:21493687 Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:39 0
pNH2
 
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RRID:Addgene_40853 repeat NH2 Xanthomonas oryzae Tetracycline PMID:21493687 Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:39 0
pNH8
 
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RRID:Addgene_40859 repeat NH8 Xanthomonas oryzae Tetracycline PMID:21493687 Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:39 0
pSB77
 
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RRID:Addgene_19590 thiosulfate reductase S. enterica serovar Typhimurium LT2 Tetracycline PMID:10966412 Backbone Marker:JM Blatny; Backbone Size:0; Vector Backbone:pJB866; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:11:46 0
pGPS21loxFRTNeo
 
Resource Report
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RRID:Addgene_27180 lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R synthetic Tetracycline PMID:15784610 The plasmid confers Kan resistance (25ug/ml) and Tet resistance (12.5ug/ml). However, it requires pir factor for replication. Note that this plasmid is supplied on a stab with only Tet. When re-plating, end users should add Kan as well. Also note that the plasmid is only considered high copy in the pir-116 strain. In a WT pir strain, it is considered low copy. Backbone Marker:New England Biolabs; Backbone Size:3000; Vector Backbone:pGPS21; Vector Types:in vitro transposion; Bacterial Resistance:Tetracycline 2026-08-15 01:13:00 0
∆DUSP6
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_27977 DUSP6 Homo sapiens Tetracycline PMID:23839489 Catalytically inactive DUSP6 Backbone Marker:Thermo Scientific; Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline C293S: mutation in the phosphatase domain 2026-08-15 01:13:09 1
DUSP6
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_27975 DUSP6 Homo sapiens Tetracycline PMID:23839489 Backbone Marker:Thermo Scientific; Backbone Size:11000; Vector Backbone:pLEX-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline 2026-08-15 01:13:09 4
pLEX SC4MOL 519
 
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RRID:Addgene_27984 SC4MOL Homo sapiens Tetracycline H173Q caused by 519T->A mutation as described in He et al., J Clin Invest. 2011;121(3):976–984. PMID: 21285510 Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline H173Q mutation in SC4MOL 2026-08-15 01:13:08 0
pLEX SC4MOL
 
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RRID:Addgene_27983 SC4MOL Homo sapiens Tetracycline Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline 2026-08-15 01:13:09 0
pTGV-Express-Tet SMR5918
 
Resource Report
Resource Website
RRID:Addgene_28035 Tetracycline PMID:11483365 Note that Addgene's sequencing results found a 1 nucleotide deletion at bp# 4286 when compared with the sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function. Backbone Size:8493; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:13:10 0
pBMTBX-4
 
Resource Report
Resource Website
RRID:Addgene_26075 Tetracycline PMID:20148414 Backbone Size:5454; Vector Backbone:pBMTBX-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:12:48 0
pStart-T2
 
Resource Report
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RRID:Addgene_20347 pStart-T2 cloning vector Tetracycline PMID:18546598 Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Mouse Targeting; Bacterial Resistance:Tetracycline 2026-08-15 01:11:53 0
pDMS197
 
Resource Report
Resource Website
RRID:Addgene_43831 Tetracycline PMID:9511756 The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination. Plasmid pRE107 (Addgene plasmid #43829) was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis. To use this plasmid with bla fusions and increase the functionality of this system, the depositing laboratory replaced the ApR gene as follows. The ApR gene flanked by the remaining BamHI sites was removed and replaced with either CmR, TcR or KmR resistance markers (see associated schematic image). The TcR gene was cloned on a blunted EcoRI–StyI fragment from pBR322 into the blunted BamHI sites to give pDMS197. The BamHI site near the MCS was regenerated after cloning; the BamHI site near the 5' end of TcR/TetR was not. The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers. Moreover, the use of Tc also allows an alternative negative selection using fusaric acid and chlortetracycline as described previously (Maloy and Nunn, 1981). Backbone Marker:Addgene plasmid 43829; Backbone Size:5173; Vector Backbone:pRE107 (modified from pGP704); Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Tetracycline 2026-08-15 01:15:10 0
pStA1AB
 
Resource Report
Resource Website
RRID:Addgene_114163 Tetracycline PMID:30462270 Accession Numer MG649421, Alternative ID = pGT402 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint. Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:02:19 0
pStA1DZ
 
Resource Report
Resource Website
RRID:Addgene_114168 Tetracycline PMID:30462270 Accession Number MG649428 , Alternative ID = pGT407 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint. Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:02:19 0
pStA1BC
 
Resource Report
Resource Website
RRID:Addgene_114165 Tetracycline PMID:30462270 Accession Number MG649423, Alternative ID = pGT404 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint. Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:02:19 0
pStA1DE
 
Resource Report
Resource Website
RRID:Addgene_114169 Tetracycline PMID:30462270 Accession Number MG649427, Alternative ID = pGT408 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint. Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:02:19 0
pStA1EZ
 
Resource Report
Resource Website
RRID:Addgene_114170 Tetracycline PMID:30462270 Accession Number MG649429, Alternative ID = pGT409 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint. Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:02:20 0
pNDGG059
 
Resource Report
Resource Website
RRID:Addgene_231325 Tetracycline PMID:39919271 Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:32:57 0

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