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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725255.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145456 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725305.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145463 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145461 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 M (membrane)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724393.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145424 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 M (membrane)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724393.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145428 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR SL1 alone
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35149555
Proper citation: RRID:Addgene_191485 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35149555
Proper citation: RRID:Addgene_191480 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR ΔSL1
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35149555
Proper citation: RRID:Addgene_191484 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: MBP-Nsp1
Vector Backbone Description: Vector Backbone:pCMV-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35149555
Proper citation: RRID:Addgene_191483 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR SL1 alone
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32995777
Proper citation: RRID:Addgene_191482 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR ΔSL1
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32995777
Proper citation: RRID:Addgene_191481 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: rS2d
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35447081
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.25.477784v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_183516 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-OMICRON.BA.2
Vector Backbone Description: Backbone Size:4745; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35732171
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_184829 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-OMICRON.BA2-RBD
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35732171
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_184830 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N)
Vector Backbone Description: Backbone Size:4745; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35447081
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.25.477784v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182575 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 3C-like proteinase
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725301.1
Proper citation: RRID:Addgene_152328 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36630960
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15 (amino acid residues 6453-6798 of YP_009724389).
Used for the determination of the atomic-resolution (2.6 angstrom) structure of the room temperature form of SARS-CoV-2 NendoU by serial femtosecond crystallography at an X-ray free-electron laser (XFEL), Protein Data Bank accession number 7K9P, Jernigan et al 2023 Structure 31:1-14, https://doi.org/10.1016/j.str.2022.12.009, PMID 36630960.
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Construct design by Debra T. Hansen.
Proper citation: RRID:Addgene_187656 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-2P-N317P
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36590900
Proper citation: RRID:Addgene_196378 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-E266Q
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40411374
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-E266Q (amino acid residues 6453-6798 of YP_009724389, with E266Q mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_218803 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-D183N
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40411374
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-D183N (amino acid residues 6453-6798 of YP_009724389, with D183N mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219526 Copy
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