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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pAG416GPD-Cerulean-CHC1
 
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RRID:Addgene_18850 CHC1 Saccharomyces cerevisiae Ampicillin PMID:18162536 Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:39 0
pAG416GPD-SEC7-Cerulean
 
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RRID:Addgene_18851 SEC7 Saccharomyces cerevisiae Ampicillin PMID:18162536 Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:39 0
pBAD18HMGR
 
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RRID:Addgene_19041 truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase Saccharomyces cerevisiae Ampicillin PMID:17239639 Backbone Marker:Guzman et al.; Backbone Size:0; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:11:41 0
Dom34 K45A
 
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RRID:Addgene_19111 Protein DOM34 Saccharomyces cerevisiae Ampicillin PMID:17889667 Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin K45A 2026-08-15 01:11:42 0
Dom34 K82A
 
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RRID:Addgene_19112 Protein DOM34 Saccharomyces cerevisiae Ampicillin PMID:17889667 Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin K82A 2026-08-15 01:11:42 0
Dom34(265-386)
 
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RRID:Addgene_19102 Protein DOM34 Saccharomyces cerevisiae Kanamycin PMID:17889667 Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:11:42 0
Dom34 E261K
 
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RRID:Addgene_19109 Protein DOM34 Saccharomyces cerevisiae Ampicillin PMID:17889667 Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E261K 2026-08-15 01:11:42 0
pcDNA5/FRT/TO V5 SSA1
 
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RRID:Addgene_19516 SSA1 Saccharomyces cerevisiae Ampicillin PMID:18686016 Part of the HSP70 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene. Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:45 0
pGC92
 
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RRID:Addgene_19615 FLP Saccharomyces cerevisiae Ampicillin PMID:18723890 Backbone Size:0; Vector Backbone:pBluescript SK+; Vector Types:carries FLP recombinase cDNA; Bacterial Resistance:Ampicillin 2026-08-15 01:11:46 0
pGST-Vps4
 
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1+ mentions
RRID:Addgene_21495 VPS4 Saccharomyces cerevisiae Ampicillin PMID:19234443 Backbone Size:5037; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:12:02 3
pMBP-HIS2-Snf7
 
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1+ mentions
RRID:Addgene_21492 SNF7 Saccharomyces cerevisiae Ampicillin PMID:19234443 Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:12:02 6
pMBP-HIS2-Vps20FL
 
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1+ mentions
RRID:Addgene_21490 VPS20 Saccharomyces cerevisiae Ampicillin PMID:19234443 Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:12:02 3
PAP-delta10-cHIS
 
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RRID:Addgene_21716 polyA polymerase Saccharomyces cerevisiae Ampicillin PMID:15909992 See attached purification scheme Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:Pet21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 30 amino acids off the c terminus 2026-08-15 01:12:04 0
pNR6
 
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RRID:Addgene_21677 Ura3 Saccharomyces cerevisiae Ampicillin PMID:3065627 Constructed by inserting a HindIII URA3 fragment into the HindIII site of the multiple cloning site of plasmid pRK113. Contains the 117-bp HO cut site of MATa inserted between the HincII and BamHI sites of pUC9 and the URA3 1.17-kb HindIII fragment at the HindlIl site transcribed away from the HO cut site. Backbone Marker:R. Kostriken; Backbone Size:2700; Vector Backbone:pRK113; Vector Types:integration sequence; Bacterial Resistance:Ampicillin 2026-08-15 01:12:04 0
pES1002
 
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RRID:Addgene_21789 Fus3-as2 Saccharomyces cerevisiae Ampicillin PMID:19079053 There are several sequence discrepancies between depositor's sequence and Addgene sequence in the 5' UTR. The 5'UTR in the plasmid is from a W303-derived strain, and the SGD used S288C strain for genome sequencing, so the mismatches could be SNPs. This plasmid is functional and these mismatches do not affect the ORF. Backbone Size:4700; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin contains 5' 1kb upstream UTR/promoter Q93A mutation in open reading frame 3' 400bp downstream UTR/terminator 2026-08-15 01:12:05 0
pRY1004
 
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RRID:Addgene_21788 Dig1-YFP Saccharomyces cerevisiae Ampicillin PMID:19079053 Construction was complicated; please refer to parent article's supplemental materials for details of construction. Backbone Size:3300; Vector Backbone:pACL7-YFP-A206K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin contains 5' 1000bp UTR (Dig1 promoter) Dig1 open reading frame YFP 3' ADH terminator from pACL7-YFP-A206K 2026-08-15 01:12:05 0
pVP64
 
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RRID:Addgene_22287 REV1 Saccharomyces cerevisiae Ampicillin PMID:19528320 The REV1 ORF plus 1050 nucleotides (nt) of its upstream region that include the REV1 promoter and 500nt of the downstream region that include the REV1 transcription terminator was cloned in the vector YCplac111, which carries the LEU2 gene, the autonomous replicating sequence ARS1, and the centromeric CEN4 region. A BglII restriction site was added by site-directed mutagenesis right before the stop codon of REV1. A 33-mer oligonucleotide duplex containing the HA-coding sequence and a BamHI and a BglII restriction site was inserted into the BglII restriction site, adding an HA tag in fusion with REV1, and regenerating a unique BglII restriction site. This step was repeated three times in order to get four HA tags in fusion with REV1. Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:10 0
pR5-19
 
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1+ mentions
RRID:Addgene_22288 RAD5 Saccharomyces cerevisiae Ampicillin PMID:18757916 Mutation abolishes the ubiquitin ligase function Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin C914, C917->AA in the C3HC4 ring-finger motif 2026-08-15 01:12:10 1
pR5-28
 
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1+ mentions
RRID:Addgene_22289 RAD5 Saccharomyces cerevisiae Ampicillin PMID:18757916 Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:10 1
UBC13-PGEX4T
 
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RRID:Addgene_25464 Ubc13 Saccharomyces cerevisiae Ampicillin PMID:16980971 Backbone Size:4969; Vector Backbone:pGEX4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:44 0

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