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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
p42Neo 1-F GW
 
Resource Report
Resource Website
RRID:Addgene_58550 Chloramphenicol and Ampicillin Backbone Size:8098; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:17:17 0
p416 3-F GW
 
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RRID:Addgene_58552 Chloramphenicol and Ampicillin Backbone Size:7084; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:17:17 0
pLgw RFC1-V5-EcoDam
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_59205 Chloramphenicol and Ampicillin PMID:17038565 The V5 epitope tag serves as a linker between Dam and your favorite protein. The lentiviral vectors can be used in two ways. When transfected as a conventional plasmid, the strong viral promoter drives high expression of the Dam(fusion) proteins. This should not be used for DamID experiments, but it is useful for checking of the proteins by Western blotting or immunofluorescence microscopy. When used as lentivirus, the strong promoter is deleted upon integration of the virus, and expression of the Dam(fusion) is now driven by the weak pIND-derived heatshock promoter. Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:17:21 1
pINDgw RFA-V5-EcoDam
 
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RRID:Addgene_59204 Chloramphenicol and Ampicillin PMID:17038565 Note that this vector can not be used to express Dam only. Backbone Marker:van Steensal lab, Addgene plasmid 59201; Backbone Size:7660; Vector Backbone:pIND(V5)EcoDam; Vector Types:Mammalian Expression, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:17:21 0
pT2SC
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_59382 TT-ISceI-cat Synthetic Chloramphenicol and Ampicillin PMID:25255806 iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:17:23 1
pT2SCb
 
Resource Report
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RRID:Addgene_59385 TT-ISceI-cat2 Synthetic Chloramphenicol and Ampicillin PMID:25255806 iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. The 3'-end was modified from 5'-GGGCGGGGCGTAA to 5'-AGGAGGTGCATAA in order to provide a site resembling the consensus bacterial ribosomal binding site (5'-AGGAGGTAAATAA). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin The 3’-end of the chloramphenicol resistance gene was modified to 5’-AGGAGGTGCATAA to create a sequence resembling the consensus bacterial ribosomal binding site (5’-AGGAGGTAAATAA). 2026-08-15 01:17:23 0
pSGE_240_pAGW_V5
 
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RRID:Addgene_71188 Chloramphenicol and Ampicillin PMID:26550828 Vector Backbone:pSGE_240_pAGW_V5; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:19:09 0
pSW_1
 
Resource Report
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RRID:Addgene_71279 Chloramphenicol and Ampicillin PMID:26550828 Vector Backbone:pSW_1; Vector Types:Gateway vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:19:10 0
pSTARR-seq_fly
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_71499 Chloramphenicol and Ampicillin PMID:23328393 This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal. Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:19:11 6
pSTARR-seq_fly-pnr
 
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RRID:Addgene_71503 Chloramphenicol and Ampicillin PMID:25517091 This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal. Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:19:11 0
pSTARR-seq_fly-RpS12
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_71504 Chloramphenicol and Ampicillin PMID:25517091 This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal. Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:19:11 1
pSTARR-seq_fly-NipB
 
Resource Report
Resource Website
RRID:Addgene_71502 Chloramphenicol and Ampicillin PMID:25517091 This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal. Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:19:11 0
pGL3-DSCP-Gateway
 
Resource Report
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RRID:Addgene_71507 Chloramphenicol and Ampicillin PMID:25517091 Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:19:11 0
pGL3-RpS12-Gateway
 
Resource Report
Resource Website
RRID:Addgene_71508 Chloramphenicol and Ampicillin PMID:25517091 Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:19:11 0
pGL4-Gateway-SCP1
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_71510 Chloramphenicol and Ampicillin PMID:23328393 Note: The plasmid for enhancer-activity testing by luciferase assays has been used in the Arnold et al. 2013 publication. It should be replaced for all enhancer activity tests mammalian cells with the updated version reported by Muerdter et al. 2017. The improved version is Addgene plasmid # 99297. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017). Gateway-cassette was added between the KpnI and BglII sites and SCP1 between BglII and HindIII Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:19:11 1
LIIc R 1-2
 
Resource Report
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RRID:Addgene_54367 Chloramphenicol and Ampicillin PMID:24551083 binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:16:39 0
LIIc F 1-2
 
Resource Report
Resource Website
RRID:Addgene_54366 Chloramphenicol and Ampicillin PMID:24551083 binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:16:39 0
LIIc F 1-2 RNAi
 
Resource Report
Resource Website
RRID:Addgene_54402 Chloramphenicol and Ampicillin PMID:24551083 ihpRNA silencing vector (cloning only) Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:16:40 0
LIIc R 5-6
 
Resource Report
Resource Website
RRID:Addgene_54373 Chloramphenicol and Ampicillin PMID:24551083 binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:16:39 0
pLEX_305-N-dTAG
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_91797 Chloramphenicol and Ampicillin PMID:29581585 Backbone Marker:David Root Lab (addgene #41390); Backbone Size:9621; Vector Backbone:pLEX_305; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:22:24 20

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