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Vector Backbone Description: Vector Backbone:pAAV-Neo_CAG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41372705
Comments: pAAV-Neo_CAG is an empty AAVS1-targeting backbone used as a filler plasmid during genome editing. It carries a CAG-driven neomycin resistance cassette and is co-delivered with pAAV-Puro_siKD2.0 (Plasmid #220536) during nucleofection to enrich for biallelic AAVS1 integration. This plasmid does not express any shRNA or functional payload and serves only as a selection-compatible filler vector.
Please visit https://dx.doi.org/10.17504/protocols.io.e6nvw4q29lmk/v1 for a detailed protocol for use.
Proper citation: RRID:Addgene_220537 Copy
Species: Homo sapiens
Genetic Insert: ZNF865 gRNA
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: The BioRxiv pre-print is available at: https://www.biorxiv.org/content/10.1101/2025.06.13.659603v2
This plasmid was adapted from addgene #83919 with a ZNF865 gRNA clone in between esp3I sites.
Proper citation: RRID:Addgene_241309 Copy
Species: Homo sapiens
Genetic Insert: ZNF865 sgRNA
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: The BioRxiv pre-print is available at: https://www.biorxiv.org/content/10.1101/2025.06.13.659603v2
This plasmid was adapted from addgene #71237 with a ZNF865 gRNA cloned in between esp3I sites.
Proper citation: RRID:Addgene_241308 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR1-mRuby-EF1a-CMVe_MeP-EGFP-MRE1_4×9
Vector Backbone Description: Backbone Size:3041; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218088 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR5-mRuby-EF1a-CMVe_MeP-EGFP-MRE5_4×12
Vector Backbone Description: Backbone Size:3048; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218242 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR5-mRuby-EF1a-CMVe_MeP-EGFP-MRE5_4×8
Vector Backbone Description: Backbone Size:3048; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218240 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR4-mRuby-EF1a-CMVe_MeP-EGFP-MRE4_4×8
Vector Backbone Description: Backbone Size:3048; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218237 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR2-mRuby-EF1a-CMVe_MeP-EGFP-MRE2_4×9
Vector Backbone Description: Backbone Size:3041; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218090 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR2-mRuby-EF1a-CMVe_MeP-EGFP-MRE2_8×8
Vector Backbone Description: Backbone Size:3043; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218091 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR5-mRuby-EF1a-CMVe_MeP-EGFP-MRE5_8×8
Vector Backbone Description: Backbone Size:3048; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218243 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR10-mRuby-EF1a-CMVe_MeP-EGFP-MRE10_8×9
Vector Backbone Description: Backbone Size:3041; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218261 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-EGFP-MREL_4×17
Vector Backbone Description: Backbone Size:3041; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218262 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR9-mRuby-EF1a-CMVe_MeP-EGFP-MRE9_4×16
Vector Backbone Description: Backbone Size:3041; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218257 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR8-mRuby-EF1a-CMVe_MeP-EGFP-MRE8_4×8
Vector Backbone Description: Backbone Size:3041; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218254 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR9-mRuby-EF1a-CMVe_MeP-EGFP-MRE9_4×17
Vector Backbone Description: Backbone Size:3041; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218258 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiR9-mRuby-EF1a-CMVe_MeP-EGFP-MRE9_4×18
Vector Backbone Description: Backbone Size:3041; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218259 Copy
Species: Mus musculus
Genetic Insert: CKS2
Vector Backbone Description: Backbone Marker:David Root, Addgene plasmid #41393; Backbone Size:7597; Vector Backbone:pCW; Vector Types:Mammalian Expression, Lentiviral, Dox-inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41200793
Comments: Please visit https://doi.org/10.1101/2025.06.06.657372 for bioRxiv preprint.
Proper citation: RRID:Addgene_247439 Copy
Species: Mus musculus
Genetic Insert: MRTF-A
Vector Backbone Description: Backbone Marker:David Root, Addgene plasmid #41393; Backbone Size:7597; Vector Backbone:pCW; Vector Types:Mammalian Expression, Lentiviral, Dox-inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41200793
Comments: Please visit https://doi.org/10.1101/2025.06.06.657372 for bioRxiv preprint.
Proper citation: RRID:Addgene_247436 Copy
Species: Homo sapiens
Genetic Insert: RhoA(G14V)
Vector Backbone Description: Backbone Marker:David Root, Addgene plasmid #41393; Backbone Size:7597; Vector Backbone:pCW; Vector Types:Mammalian Expression, Lentiviral, Dox-inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41200793
Comments: Please visit https://doi.org/10.1101/2025.06.06.657372 for bioRxiv preprint.
Proper citation: RRID:Addgene_247437 Copy
Species: Homo sapiens
Genetic Insert: synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-EGFR-mEGFP-MREL_4×19
Vector Backbone Description: Backbone Size:3029; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_218267 Copy
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