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Species: Arabidopsis thaliana
Genetic Insert: SEIPIN2
Vector Backbone Description: Backbone Size:12460; Vector Backbone:pMDC43; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26362606
Comments: The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html
Proper citation: RRID:Addgene_96977 Copy
Species: Arabidopsis thaliana
Genetic Insert: SEIPIN1
Vector Backbone Description: Backbone Size:12460; Vector Backbone:pMDC84; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26362606
Comments: The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html
Proper citation: RRID:Addgene_96979 Copy
Species: Arabidopsis thaliana
Genetic Insert: SEIPIN3
Vector Backbone Description: Backbone Size:12460; Vector Backbone:pMDC84; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26362606
Comments: The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html
Proper citation: RRID:Addgene_96982 Copy
Species: Arabidopsis thaliana
Genetic Insert: SEIPIN1
Vector Backbone Description: Backbone Size:12460; Vector Backbone:pMDC84; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26362606
Comments: The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html
Proper citation: RRID:Addgene_96983 Copy
Species: Arabidopsis thaliana
Genetic Insert: SEIPIN1
Vector Backbone Description: Backbone Size:7840; Vector Backbone:pRS315-PGK; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26362606
Comments: Please note that mutations V69A and D300N in LEU2 were found during Addgene's quality control process. The depositor noted that these mutations do NOT affect plasmid function.
Proper citation: RRID:Addgene_96986 Copy
Species: Arabidopsis thaliana
Genetic Insert: phytochrome interacting factor 3
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pL1A-lc; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28911120
Proper citation: RRID:Addgene_98744 Copy
Species: Arabidopsis thaliana
Genetic Insert: CmYLCV:Luc+:HSP
Vector Backbone Description: Vector Backbone:pMOD_A4800EC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176878 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUbi10:Luc+:RbcsE9
Vector Backbone Description: Vector Backbone:pMOD_C'4800EC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176879 Copy
Species: Arabidopsis thaliana
Genetic Insert: HSP terminator
Vector Backbone Description: Vector Backbone:pICH41276; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176882 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtAFB2
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6390; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: This lentiviral plasmids delivers AtAFB2 (auxin-inducible degron system) fused to mTagBFP2. In combination with TetOn3G can be used to generate DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179889 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA cassette under U3 promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35022409
Comments: This vector was designed and constructed by Dr. Tomasz Bieluszewski.
Detailed protocol for CRISPR/Cas9-based mutagenesis using this system in:
Bieluszewski T, Szymanska-Lejman M, Dziegielewski W, Zhu L, Ziolkowski PA. Efficient Generation of CRISPR/Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions, in: Plant Gametogenesis: Methods and Protocols (ed. Christophe Lambing), Methods in Molecular Biology, vol. 2484.
https://doi.org/10.1007/978-1-0716-2253-7_13
https://pubmed.ncbi.nlm.nih.gov/35461452/
Proper citation: RRID:Addgene_173156 Copy
Species: Arabidopsis thaliana
Genetic Insert: DHN_AtCor47
Vector Backbone Description: Vector Backbone:YK 02; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35176859
Comments: This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). See supplemental table 2 in our paper to see its performance in our assay. The negative control for this assay is pSecTag2 B which can be obtained from Thermo here https://www.thermofisher.com/order/catalog/product/V90020
Proper citation: RRID:Addgene_185254 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-GFP-FUSN
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183932 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-GFP-FUSN-VP16
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183933 Copy
Species: Arabidopsis thaliana
Genetic Insert: tdPCP-CIBN
Vector Backbone Description: Backbone Marker:Addgene #54642; Backbone Size:4592; Vector Backbone:tdTomato-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Comments: under control of the weakened deltaTATA-CMV promoter for low level expression
Proper citation: RRID:Addgene_183937 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN-rTetR
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183919 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-GFP-p65
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183929 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-GFP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183926 Copy
Species: Arabidopsis thaliana
Genetic Insert: AFB2
Vector Backbone Description: Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Digest vector with EcoRV for integration into TRP1.
Proper citation: RRID:Addgene_99531 Copy
Species: Arabidopsis thaliana
Genetic Insert: TIR1
Vector Backbone Description: Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Digest this vector with EcoRV for integration into TRP1.
Addgene NGS results found a single nucleotide change compared to NM_116163.4 that results in T591I within the TIR1 translation. The protein is noted by the depositing laboratory to be functional in vivo
Proper citation: RRID:Addgene_99532 Copy
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