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Species: Other
Genetic Insert: dnaQ926 dnaX2016
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69637 Copy
Species: Other
Genetic Insert: dnaQ926 dnaX36
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69636 Copy
Species: Other
Genetic Insert: dnaQ926 dnaE1026
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69635 Copy
Genetic Insert: Snap-Omp25
Vector Backbone Description: Backbone Marker:Jason Moffat; Vector Backbone:pLJM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25837514
Proper citation: RRID:Addgene_69599 Copy
Species: Rattus norvegicus
Genetic Insert: paGFP-Omp25
Vector Backbone Description: Backbone Marker:Jason Moffat; Backbone Size:7327; Vector Backbone:pLJM2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25837514
Proper citation: RRID:Addgene_69598 Copy
Species: Other
Genetic Insert: rpsD16
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69649 Copy
Species: Other
Genetic Insert: rpsD14
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69648 Copy
Species: Other
Genetic Insert: rpsD12
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69647 Copy
Species: Other
Genetic Insert: dnaQ926 mutH(K116E)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69646 Copy
Species: Other
Genetic Insert: dnaQ926 mutH(K79E)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69645 Copy
Species: Other
Genetic Insert: dnaQ926 mutL713
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69641 Copy
Species: Homo sapiens
Genetic Insert: HPRT gRNA2
Vector Backbone Description: Vector Backbone:pGEM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26130722
Proper citation: RRID:Addgene_69539 Copy
Species: Other
Genetic Insert: dnaQ926 dam
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69650 Copy
Vector Backbone Description: Vector Backbone:GGdest; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26352799
Proper citation: RRID:Addgene_69538 Copy
Genetic Insert: 5 concatenated gRNA transcriptional cassettes targeting OCT4
Vector Backbone Description: Vector Backbone:GGdest; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26352799
Proper citation: RRID:Addgene_69537 Copy
Species: Mus musculus
Genetic Insert: Myogenin C terminus-targeting guide RNA
Vector Backbone Description: Vector Backbone:pZDonor; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Compatible with S. pyogenes Cas9.
Proper citation: RRID:Addgene_69549 Copy
Species: Synthetic
Genetic Insert: 2A-mCherry-NLS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:PCR2.1; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69548 Copy
Species: Synthetic
Genetic Insert: 2A-mCherry-NLS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:PCR2.1; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69547 Copy
Species: Mus musculus
Genetic Insert: mSTIM1_1-250
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26184105
Proper citation: RRID:Addgene_69552 Copy
Species: Mus musculus
Genetic Insert: mSTIM1_1-310
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26184105
Proper citation: RRID:Addgene_69551 Copy
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