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Species: Mus musculus
Genetic Insert: minimal FGF4 promoter
Vector Backbone Description: Backbone Marker:Lois and Baltimore (ADDGENE # 14882); Backbone Size:9206; Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24658142
Comments: Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_69443 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26322384
Proper citation: RRID:Addgene_69616 Copy
Species: Synthetic
Genetic Insert: kozak consensus - N-CretrcintG
Vector Backbone Description: Backbone Size:4823; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26258682
Proper citation: RRID:Addgene_69572 Copy
Species: E. coli
Genetic Insert: aac
Vector Backbone Description: Backbone Size:2140; Vector Backbone:pSG5; Vector Types:; Bacterial Resistance:Apramycin
Defining Citation: PMID:30191290
Proper citation: RRID:Addgene_69615 Copy
Species: Transposon Tn5
Genetic Insert: aphII
Vector Backbone Description: Backbone Size:2140; Vector Backbone:pSG5; Vector Types:Bacterial Expression, Streptomyces- E. coli shuttle vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16776656
Comments: Stable replicating, multi copy shuttle vector for thiostrepton induced gene expression in Streptomyces
temperature sensitive replication (G. Muth, B. Nußbaumer, W. Wohlleben and A. Pühler (1989) A vector system with temperature-sensitive replication for gene disruption and mutational cloning in streptomycetes. Mol. Gen. Genet. 219, 341-348 )
http://www.uni-tuebingen.de/en/faculties/faculty-of-science/departments/inter-faculty-institutes-and-centres/imit/units/mikrobiologiebiotechnologie/work-groups/muth/plasmids.html
Proper citation: RRID:Addgene_69614 Copy
Species: Homo sapiens
Genetic Insert: L1PA13A
Vector Backbone Description: Backbone Size:3530; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The ORF1 and ORF2 of the codon optimized L1PA13A element were commercially synthetically generated and then cloned into the parental pBS-L1PA1CHmneo by swapping the two ORFs.
Proper citation: RRID:Addgene_69613 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24006363
Comments: The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3.
Proper citation: RRID:Addgene_69579 Copy
Species: Homo sapiens
Genetic Insert: L1PA1
Vector Backbone Description: Backbone Size:3530; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69612 Copy
Species: Homo sapiens
Genetic Insert: L1PA1
Vector Backbone Description: Backbone Size:3530; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The blast rescue cassette was commercially synthesized and cloned into the EcoRI-NotI sites of L1PA1CHmneo to swap the mneo cassette with the blast rescue cassette sequence
Proper citation: RRID:Addgene_69611 Copy
Species: Other
Genetic Insert: dnaE74
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69629 Copy
Species: Other
Genetic Insert: dnaQ926 umuD' umuC recA730
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69627 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25344068
Comments: The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3. This is tagged with the N-terminus of a split mVenus.
Proper citation: RRID:Addgene_69582 Copy
Species: Homo sapiens
Genetic Insert: p53-L344P
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24006363
Comments: This mutant will not dimerize nor tetramerize. The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3.
Proper citation: RRID:Addgene_69581 Copy
Species: Homo sapiens
Genetic Insert: p53-L344A
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24006363
Comments: This mutant will dimerize, but not tetramerize. The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3.
Proper citation: RRID:Addgene_69580 Copy
Species: Synthetic
Genetic Insert: cp173Venus-Ggamma2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26799488
Proper citation: RRID:Addgene_69626 Copy
Species: Synthetic
Genetic Insert: Gbeta-2A-cpV-Ggamma2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26799488
Proper citation: RRID:Addgene_69625 Copy
Species: Synthetic
Genetic Insert: Galphai1-mTurquoise2
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26799488
Proper citation: RRID:Addgene_69620 Copy
Species: Homo sapiens
Genetic Insert: p53-L344P
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25344068
Comments: This mutant will not dimerize nor tetramerize. This p53 is tagged with the N-term of a split mVenus. The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3.
Proper citation: RRID:Addgene_69586 Copy
Species: Other
Genetic Insert: dnaQ926 mutS538'
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69638 Copy
Species: Mus musculus
Genetic Insert: TCR beta VJ region from clone 1.1
Vector Backbone Description: Backbone Marker:Carbone Lab, Addgene plasmid 69600; Vector Backbone:p3A9CbetaTCR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9553774
Proper citation: RRID:Addgene_69592 Copy
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