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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pZS1[GalS-L,FruR-L]
 
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RRID:Addgene_60764 GalS-L Synthetic Ampicillin PMID:25035932 GalS-L and FruR-L also described as LLhS_Q54A and LLhF_Q60S respectively in PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013. Rheostats and toggle switches for modulating protein function. Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L. Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin LacI/GalR repressor chimera. LacI DBD with GalS LBD. Contains point mutation Q54A, E191K 2026-08-15 01:17:36 0
pZS1[GalS-L,RbsR-L]
 
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RRID:Addgene_60763 GalS-L Synthetic Ampicillin PMID:25035932 GalS-L and RbsR-L also described as LLhS_Q54A and LLhR_Q60A respectively in PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013. Rheostats and toggle switches for modulating protein function. Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L. Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin LacI/GalR repressor chimera. LacI DBD with GalS LBD. Contains point mutation Q54A, E191K 2026-08-15 01:17:35 0
pZS1[FruR-L,RbsR-L]
 
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1+ mentions
RRID:Addgene_60761 FruR-L Synthetic Ampicillin PMID:25035932 FruR-L and RbsR-L also described as LLhF_Q60S and LLhR_Q60A respectively in PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013. Rheostats and toggle switches for modulating protein function. Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L. Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin LacI/GalR repressor chimera. LacI DBD with FruR LBD. Contains point mutation Q60S 2026-08-15 01:17:35 1
pZS1[GalS-L,TreR-L]
 
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RRID:Addgene_60765 GalS-L Synthetic Ampicillin PMID:25035932 GalS-L and TreR-L also described as LLhS_Q54A and TreR_V52A respectively in PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013. Rheostats and toggle switches for modulating protein function. Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L. Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin LacI/GalR repressor chimera. LacI DBD with GalS LBD. Contains point mutation Q54A, E191K 2026-08-15 01:17:35 0
pZS1[GalS-T,TreR-T]
 
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RRID:Addgene_60775 GalS-T Synthetic Ampicillin PMID:25035932 Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin LacI/GalR repressor chimera. TAN DBD with GalS LBD. Contains point mutation Q54A 2026-08-15 01:17:36 0
pZS1[FruR-T,TreR-T]
 
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RRID:Addgene_60772 FruR-T Synthetic Ampicillin PMID:25035932 Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin LacI/GalR repressor chimera. TAN DBD with FruR LBD. Contains point mutation Q60S, T61S 2026-08-15 01:17:36 0
pZS1[RbsR-T,TreR-T]
 
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RRID:Addgene_60776 RbsR-L Synthetic Ampicillin PMID:25035932 Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin LacI/GalR repressor chimera. TAN DBD with RbsR LBD. Contains point mutation Q60A 2026-08-15 01:17:36 0
pCMV5-25BoxB
 
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1+ mentions
RRID:Addgene_60817 25x BoxB Synthetic Ampicillin PMID:24035393 the authors of Gehring et al. Mol Cell. 2003 Apr;11(4):939-49 were contacted by us and granted their permission to distribute the 25xboxb construct generated in our lab from their precursor for non-profit purpose via Addgene Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 25 repeats of BoxB sequence 2026-08-15 01:17:36 2
pTriEx-mCherry::LANS4
 
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1+ mentions
RRID:Addgene_60785 LANS4 Synthetic Ampicillin PMID:26083500 Backbone Size:5943; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:36 4
pFA6a-link-GFPγ-SpHis5
 
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RRID:Addgene_60781 GFPγ Synthetic Ampicillin PMID:25612242 Backbone Size:4021; Vector Backbone:pFA6a; Vector Types:Yeast Integrating; Bacterial Resistance:Ampicillin T65C S72A V163A I167T L231H 2026-08-15 01:17:36 0
pTSlb-wt
 
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RRID:Addgene_60728 Residues 1-179 of split T7 RNAP Synthetic Chloramphenicol PMID:23479654 Backbone Size:5500; Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Residues 1-180 of split T7 RNAP 2026-08-15 01:17:35 0
pTSara-R756K
 
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RRID:Addgene_60727 Residues 1-179 of split T7 RNAP Synthetic Chloramphenicol PMID:23479654 Backbone Size:5500; Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Residues 1-180 of split T7 RNAP 2026-08-15 01:17:35 0
pTSlb-R756S
 
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RRID:Addgene_60730 Residues 1-179 of split T7 RNAP Synthetic Chloramphenicol PMID:23479654 Backbone Size:5500; Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Residues 1-180 of split T7 RNAP 2026-08-15 01:17:35 0
pzs1[GalS-L]
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_60743 GalS-L Synthetic Ampicillin PMID:25035932 GalS-L also described as LLhS_Q54A in PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013. Rheostats and toggle switches for modulating protein function. Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L. Backbone Marker:Expressys; Backbone Size:4000; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin LacI/GalR repressor chimera. LacI DBD with GalS LBD. Contains point mutation Q54A, E191K 2026-08-15 01:17:35 1
single polypeptide FPX biosensor for caspase-1
 
Resource Report
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RRID:Addgene_60885 single polypeptide FPX biosensor for caspase-1 Synthetic Ampicillin PMID:25622108 Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:37 0
single polypeptide FPX biosensor for caspase-3
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_60883 single polypeptide FPX biosensor for caspase-3 Synthetic Ampicillin PMID:25622108 Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:37 1
pPHT-PIP2
 
Resource Report
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RRID:Addgene_60937 PIP2 dimerization dependent sensor RA+B monomers Synthetic Ampicillin PMID:25622108 Contains a dimerization dependent fluorescent protein, originally described by Alford, S.C., Ding, Y., Simmen, T., and Campbell, R.E. (2012). Dimerization-Dependent Green and Yellow Fluorescent Proteins. ACS Synth. Biol. 1, 569–575. Addgene NGS results identified a frameshift mutation in the HygR gene originally indicated in this plasmid. The depositing lab has confirmed that their experiments were done with transient transfection and did not make use of Hygromycin selection. For users who need a mammalian selection marker, they recommend moving the biosensor coding region from this plasmid into a different plasmid backbone. Vector Backbone:pUB2.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:37 0
pPHT-PKA
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_60936 PKA FPX Sensor Synthetic Ampicillin PMID:25622108 Contains a dimerization dependent fluorescent protein, originally described by Alford, S.C., Ding, Y., Simmen, T., and Campbell, R.E. (2012). Dimerization-Dependent Green and Yellow Fluorescent Proteins. ACS Synth. Biol. 1, 569–575. Vector Backbone:pUB2.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:37 3
PX551
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_60957 SpCas9 Synthetic Ampicillin PMID:25326897 Backbone Size:2905; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:17:37 49
GOMO-GFP-LUC
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_60976 GFP-Luciferase fusion Synthetic Ampicillin PMID:24755823 Backbone Size:2877; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:17:38 3

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