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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pEcCas5 Resource Report Resource Website |
RRID:Addgene_170090 | E. coli type I-E Cas5 | E. coli | Kanamycin | PMID:34985758 | If used in a publication, please acknowledge that the plasmid pEcCas5 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. | Backbone Size:5221; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:04 | 0 | |
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pEcCse2 Resource Report Resource Website |
RRID:Addgene_170094 | E. coli type I-E Cse2 | E. coli | Kanamycin | PMID:34985758 | If used in a publication, please acknowledge that the plasmid pEcCse2 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. | Backbone Size:5230; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:05 | 0 | |
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pIBA2-SpyTag-MBP Resource Report Resource Website |
RRID:Addgene_107422 | Maltose-binding protein (MalE) | E. coli | Ampicillin | PMID:30600549 | Spycatcher was published in Zakeri et al. (2012), PNAS 109, ppE690-7 | Backbone Marker:IBA GmbH; Backbone Size:3286; Vector Backbone:pASK-IBA2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:10 | 0 | |
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pJY019 Resource Report Resource Website |
RRID:Addgene_32999 | etpBAC locus | E. coli | Ampicillin | PMID:16552055 | Please note that the His and Myc tags are not in frame with the ORF. In addition, there is a E65G mutation in EtpB. Depositing lab does not think this mutation will affect plasmid function. | Backbone Marker:Invitrogen; Backbone Size:4094; Vector Backbone:pBAD/myc-His A; Vector Types:Bacterial Expression, n/a; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:45 | 0 | |
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pVS1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_33143 | LacO (256 copies) | E. coli | Ampicillin | PMID:18614049 | Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable. For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142). pVS2 (Addgene #33144) contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]). pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP. | Vector Backbone:Ylplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:47 | 2 | |
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EcMBP165-cpGFP.PPYF.T203V.pRSET Resource Report Resource Website |
RRID:Addgene_33373 | Improved MBP based Maltose Biosensor | E. coli | Ampicillin | PMID:21989929 | The GFP-T203V mutation decreases the fluorescence emission of the apo state and increases that of the saturated state in GCaMP2. In the context of MBP165-cpGFP.PPYF, the T203V mutation decreases the fluorescence emission of the apo state by half, whereas saturated fluorescence and affinity are unchanged, increasing ΔF/F to 6.5. | Backbone Marker:Invitrogen; Backbone Size:2300; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | cpGFP146 variant from GCaMP2 inserted between AA165-166 of MBP with T203V mutation in GFP. | 2026-08-15 01:13:49 | 0 |
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pYFJ16-LplA(W37V) (for E. Coli expression) Resource Report Resource Website 1+ mentions |
RRID:Addgene_34838 | LplA W37V | E. Coli | Ampicillin | PMID:20534555 | Backbone Marker:Gift from John Cronan; Backbone Size:4800; Vector Backbone:pYFJ16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Mutated Trp37 to Val | 2026-08-15 01:13:55 | 3 | |
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EC.RNe.TYB1.a1-400.wt Resource Report Resource Website |
RRID:Addgene_17949 | Escherichia coli RNase E residues 1-400 | E. coli | Ampicillin | PMID:16854990 | Backbone Marker:New England Biolabs; Backbone Size:7280; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | residues 1-400 | 2026-08-15 01:11:24 | 0 | |
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CP2-TCC Resource Report Resource Website |
RRID:Addgene_179679 | [sd2] T7 RNAP-TCC linker-degron | E. coli | Spectinomycin | PMID:35379961 | Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:11:23 | 0 | ||
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pEF/myc/ER FLIPglu-170nDelta13V Resource Report Resource Website |
RRID:Addgene_18022 | FLIPglu F16F Delta13 | E. coli | Ampicillin | PMID:20354141 | Backbone Marker:Invitrogen; Backbone Size:5603; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | WT binding protein (no F16A) | 2026-08-15 01:11:25 | 0 | |
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pcDNA3.1 FLIPglu-600uDelta13V Resource Report Resource Website |
RRID:Addgene_18014 | FLIPglu F16A Delta13 | E. coli | Ampicillin | PMID:20354141 | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | F16A in binding protein | 2026-08-15 01:11:24 | 0 | |
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pcDNA3.1 FLIPglu-30uDelta13V Resource Report Resource Website 1+ mentions |
RRID:Addgene_18015 | FLIPglu D154A Delta13 | E. coli | Ampicillin | PMID:20354141 | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mglB-D154A | 2026-08-15 01:11:25 | 1 | |
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KTK_032 Resource Report Resource Website |
RRID:Addgene_180528 | pLac promoter | E. coli | Ampicillin | PMID:34767345 | Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full | Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:27 | 0 | |
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pBEL2308 Resource Report Resource Website |
RRID:Addgene_181818 | LetB (a.k.a. b1834, yebT, ECK1833) | E. coli | Ampicillin | PMID:35077766 | Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | LetB(43-877; delta392-515)-6xHis | 2026-08-15 01:11:29 | 0 | |
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pBEL2309 Resource Report Resource Website |
RRID:Addgene_181819 | LetB (a.k.a. b1834, yebT, ECK1833) | E. coli | Ampicillin | PMID:35077766 | Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | LetB(43-877; delta516-633)-6xHis | 2026-08-15 01:11:29 | 0 | |
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pBEL1749 Resource Report Resource Website |
RRID:Addgene_181820 | LetB (a.k.a. b1834, yebT, ECK1833) | E. coli | Ampicillin | PMID:35077766 | Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | LetB(43-877; delta747-877)-6xHis | 2026-08-15 01:11:29 | 0 | |
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pOSIP-KL-mcherry Resource Report Resource Website |
RRID:Addgene_116947 | mcherry | E. coli | Kanamycin | PMID:29765036 | Vector Backbone:pOSIP-KL; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:49 | 0 | ||
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pLA230 Resource Report Resource Website 1+ mentions |
RRID:Addgene_11724 | b-lactamase | E. coli | Kanamycin | PMID:11602576 | Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance. The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI. Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US . | Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | GAA for Glu26 changed to TAA (premature stop codon introduced in beta-lactamase gene). | 2026-08-15 01:02:52 | 1 |
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N229A Lo-MetQ Resource Report Resource Website |
RRID:Addgene_118268 | MetQ (N229A) | E. coli | Ampicillin | PMID:30352853 | Backbone Marker:Novagen; Backbone Size:5652; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Changed Asparagine (Asn) 229 to Alanine (Ala) | 2026-08-15 01:03:01 | 0 | |
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N295A (EcN), F103A (EcI) MetNIQ in pBAD Resource Report Resource Website |
RRID:Addgene_118258 | MetN (N295A) | E. coli | Ampicillin | PMID:30352853 | delta metNIQ E. coli strain BW2115 was a created in the Rees lab by modifying E. coli genomic DNA | Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Changed Glutamic Acid (Glu) 295 to Alanine (Ala) | 2026-08-15 01:03:02 | 0 |
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