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Species: Saccharomyces cerevisiae
Genetic Insert: Med21 aa1-132 for N-terminal tagging
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26183 Copy
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:13844; Vector Backbone:pDW1714; Vector Types:Yeast Expression, reporter; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:21493687
Comments: Cloning nuclease targets into the BglII/SpeI sites of pCP5 removes the CcdB gene, thus allows negative selection against undigested backbone in common E. coli strains such as DH5-alpha.
Proper citation: RRID:Addgene_35397 Copy
Species: Vibrio fischeri
Genetic Insert: ECFP, EYFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6724; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors.
Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings
https://www.nature.com/articles/npre.2008.1550.2
Proper citation: RRID:Addgene_24509 Copy
Species: Aequorea victoria (Jellyfish)
Genetic Insert: Yn, Yc, ECFP, EYFP
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:7434; Vector Backbone:pVitro2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors.
Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings
Proper citation: RRID:Addgene_24510 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmcherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:20169111
Comments: Please note: the orientation of the MCS within this plasmid places mCherry on the c-terminus of your gene of interest.
Proper citation: RRID:Addgene_31907 Copy
Vector Backbone Description: Backbone Size:12096; Vector Backbone:pMDC32; Vector Types:Gateway destination binary vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19000159
Proper citation: RRID:Addgene_32078 Copy
Vector Backbone Description: Backbone Size:12767; Vector Backbone:pMDC32; Vector Types:Gateway destination binary vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19000159
Proper citation: RRID:Addgene_32077 Copy
Vector Backbone Description: Backbone Size:4492; Vector Backbone:pMV261; Vector Types:Bacterial Expression, Mycobacteria expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19647016
Comments: Replicative mycobacterial destination vector for recombination cloning.
Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.
Proper citation: RRID:Addgene_29463 Copy
Vector Backbone Description: Backbone Size:4445; Vector Backbone:pMV361; Vector Types:Bacterial Expression, Mycobacteria expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19647016
Comments: Integrative mycobacterial destination vector for recombination cloning.
Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.
Proper citation: RRID:Addgene_29462 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7142; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:21375737
Comments: Generic promoter-less destination vector
Proper citation: RRID:Addgene_29496 Copy
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:4774; Vector Backbone:PDONR-221 P1-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:17878951
Comments: Backbone was modified with ApaI (attP3) / EcoRI (attP5) and PstI (attP3) / EcoRV (attP5) sites
Proper citation: RRID:Addgene_186350 Copy
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:4768; Vector Backbone:PDONR-221 P1-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:17878951
Comments: Backbone was modified with ApaI (attP3) / EcoRI (attP4) and PstI (attP3) / EcoRV (attP4) sites
Proper citation: RRID:Addgene_186351 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.
Proper citation: RRID:Addgene_199083 Copy
Species: Synthetic
Genetic Insert: NPTII
Vector Backbone Description: Backbone Marker:Yiping Qi; Vector Backbone:pYPQ210; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:37070560
Comments: A 185T mutation was found in mCherry.
Proper citation: RRID:Addgene_199721 Copy
Species: Rice
Genetic Insert: miR5338
Vector Backbone Description: Backbone Marker:CAMBIA Company; Backbone Size:11827; Vector Backbone:pCAMBIA-1301; Vector Types:Bacterial Expression, Yeast Expression, Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:22345490
Comments: This plasmid used to disturb miR5338’s expression by STTM method invented by Dr. Guiliang Tang’s Lab in Michigan Technological University (Plant cell, 2012), Houghton, MI, USA. The method used to construct this plasmid refers to Tang et al., (Methods, 2012) with minor revision. The STTM sequence in this plasmid may contain discrepancies compared to the reference sequence(s). According to the depositing lab, these mutations should not affect plasmid function. For additional published information regarding this plasmid, please see https://www.cell.com/molecular-plant/pdf/S1674-2052(18)30275-2.pdf?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1674205218302752%3Fshowall%3Dtrue#
Proper citation: RRID:Addgene_84236 Copy
Species: Rice
Genetic Insert: miR5144
Vector Backbone Description: Backbone Marker:CAMBIA Company ; Backbone Size:11827; Vector Backbone:pCAMBIA-1301; Vector Types:Bacterial Expression, Yeast Expression, Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:22345490
Comments: This plasmid used to disturb miR5144’s expression by STTM method invented by Dr. Guiliang Tang’s Lab in Michigan Technological University (Plant cell, 2012), Houghton, MI, USA. The method used to construct this plasmid refers to Tang et al., (Methods, 2012) with minor revision. The STTM sequence in this plasmid may contain discrepancies compared to the reference sequence(s). According to the depositing lab, these mutations should not affect plasmid function. For additional published information regarding this plasmid, please see https://www.cell.com/molecular-plant/pdf/S1674-2052(18)30275-2.pdf?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1674205218302752%3Fshowall%3Dtrue#
Proper citation: RRID:Addgene_84235 Copy
Species: Rice
Genetic Insert: miR5794
Vector Backbone Description: Backbone Marker:CAMBIA Company; Backbone Size:11827; Vector Backbone:pCAMBIA-1301; Vector Types:Bacterial Expression, Yeast Expression, Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:22345490
Comments: This plasmid used to disturb miR5794’s expression by STTM method invented by Dr. Guiliang Tang’s Lab in Michigan Technological University (Plant cell, 2012), Houghton, MI, USA. The method used to construct this plasmid refers to Tang et al., (Methods, 2012) with minor revision. The STTM sequence in this plasmid may contain discrepancies compared to the reference sequence(s). According to the depositing lab, these mutations should not affect plasmid function. For additional published information regarding this plasmid, please see https://www.cell.com/molecular-plant/pdf/S1674-2052(18)30275-2.pdf?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1674205218302752%3Fshowall%3Dtrue#
Proper citation: RRID:Addgene_84237 Copy
Species: Rice
Genetic Insert: miR169
Vector Backbone Description: Backbone Marker:CAMBIA Company; Backbone Size:11827; Vector Backbone:pCAMBIA-1301; Vector Types:Bacterial Expression, Yeast Expression, Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:22345490
Comments: This plasmid used to disturb miR169’s expression by STTM method invented by Dr. Guiliang Tang’s Lab in Michigan Technological University (Plant cell, 2012), Houghton, MI, USA. The method used to construct this plasmid refers to Tang et al., (Methods, 2012) with minor revision. The STTM sequence in this plasmid may contain discrepancies compared to the reference sequence(s). According to the depositing lab, these mutations should not affect plasmid function. For additional published information regarding this plasmid, please see https://www.cell.com/molecular-plant/pdf/S1674-2052(18)30275-2.pdf?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1674205218302752%3Fshowall%3Dtrue#
Proper citation: RRID:Addgene_84199 Copy
Species: Rice
Genetic Insert: miR167
Vector Backbone Description: Backbone Marker:CAMBIA Company; Backbone Size:11827; Vector Backbone:pCAMBIA-1301; Vector Types:Bacterial Expression, Yeast Expression, Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:22345490
Comments: This plasmid used to disturb miR166’s expression by STTM method invented by Dr. Guiliang Tang’s Lab in Michigan Technological University (Plant cell, 2012), Houghton, MI, USA. The method used to construct this plasmid refers to Tang et al., (Methods, 2012) with minor revision The STTM sequence in this plasmid may contain discrepancies compared to the reference sequence(s). According to the depositing lab, these mutations should not affect plasmid function. For additional published information regarding this plasmid, please see https://www.cell.com/molecular-plant/pdf/S1674-2052(18)30275-2.pdf?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1674205218302752%3Fshowall%3Dtrue#
Proper citation: RRID:Addgene_84198 Copy
Species: Rice
Genetic Insert: miR1430
Vector Backbone Description: Backbone Marker:CAMBIA Company; Backbone Size:11827; Vector Backbone:pCAMBIA-1301; Vector Types:Bacterial Expression, Yeast Expression, Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:22345490
Comments: This plasmid used to disturb miR1430’s expression by STTM method invented by Dr. Guiliang Tang’s Lab in Michigan Technological University (Plant cell, 2012), Houghton, MI, USA. The method used to construct this plasmid refers to Tang et al., (Methods, 2012) with minor revision The STTM sequence in this plasmid may contain discrepancies compared to the reference sequence(s). According to the depositing lab, these mutations should not affect plasmid function. For additional published information regarding this plasmid, please see https://www.cell.com/molecular-plant/pdf/S1674-2052(18)30275-2.pdf?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1674205218302752%3Fshowall%3Dtrue#
Proper citation: RRID:Addgene_84231 Copy
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