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Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:6556; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Folding indicator GFP
Proper citation: RRID:Addgene_47070 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47071 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5780; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47068 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23145123
Proper citation: RRID:Addgene_47539 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48862 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48861 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48860 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48856 Copy
Vector Backbone Description: Backbone Marker:Imre Berger; Backbone Size:6992; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26043233
Comments: Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49050 Copy
Vector Backbone Description: Backbone Marker:Imre Berger; Backbone Size:6986; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26043233
Comments: Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49049 Copy
Vector Backbone Description: Backbone Marker:Dankort lab, unpublished; Backbone Size:7435; Vector Backbone:gQxiPuro; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48991 Copy
Vector Backbone Description: Backbone Marker:Lorenz et al, 1996 (PubMed ID: 8917596); Backbone Size:5306; Vector Backbone:pTLN; Vector Types:Xenopus oocytes expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Iwan Zimmermann, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49033 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6578; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49031 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5855; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49028 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8362; Vector Backbone:pYES2/CT; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
The ampicillin resistance cassette is located at bp# 4361-5221.
Proper citation: RRID:Addgene_49027 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7634; Vector Backbone:pYES2/CT; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
The ampicillin resistance cassette is located at bp# 3633-4493.
Proper citation: RRID:Addgene_49026 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8347; Vector Backbone:pYES2/CT; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
The ampicillin resistance cassette is located at bp# 6364-7224.
Proper citation: RRID:Addgene_49025 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7638; Vector Backbone:pYES2/CT; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
The ampicillin resistance cassette is located at bp# 3637-4497.
Proper citation: RRID:Addgene_49024 Copy
Vector Backbone Description: Backbone Marker:Dankort lab, unpublished; Backbone Size:7396; Vector Backbone:gQxiPuro; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48958 Copy
Species: Homo sapiens
Genetic Insert: Sox2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3900; Vector Backbone:pLP-TRE2; Vector Types:Mammalian Expression, Cre/Lox, donor vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
Proper citation: RRID:Addgene_49392 Copy
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