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Species: Synthetic
Genetic Insert: optimized TetR
Vector Backbone Description: Vector Backbone:pUC18T-mini-Tn7T-P3-luxABCDE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40595632
Comments: Please visit https://doi.org/10.1101/2024.09.01.610675 for bioRxiv preprint.
S17-1λpir strain genotype: TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir
Proper citation: RRID:Addgene_226434 Copy
Species: Other
Genetic Insert: SpyCatcher:mTFP1
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225189 Copy
Species: Other
Genetic Insert: SpyCatcher:mKate2
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225188 Copy
Species: Other
Genetic Insert: SpyCatcher:AausFP1
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225186 Copy
Species: Other
Genetic Insert: SpyCatcher:mNeonGreen
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225185 Copy
Species: Other
Genetic Insert: PrecA:mNeonGreen
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225192 Copy
Species: Other
Genetic Insert: SnoopCatcher:mNeonGreen
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225191 Copy
Species: Other
Genetic Insert: SpyCatcher003:mNeonGreen
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225190 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234583 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234584 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234585 Copy
Species: Homo sapiens
Genetic Insert: EED
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234576 Copy
Species: Synthetic
Genetic Insert: SpyTag-noro-VLP
Vector Backbone Description: Backbone Marker:Gibco; Backbone Size:5238; Vector Backbone:pFastBac Dual; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:33468139
Comments: Original publication:
SpyCatcher:
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. PMID: 22366317; PMCID: PMC3311370.
Proper citation: RRID:Addgene_165989 Copy
Genetic Insert: dCas9, sgRNA expression cassette, sfGFP
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38126769
Proper citation: RRID:Addgene_208889 Copy
Genetic Insert: dCas9, sgRNA expression cassette
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38126769
Proper citation: RRID:Addgene_208890 Copy
Species: Scytonema hofmanni
Genetic Insert: tnsB
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.
Proper citation: RRID:Addgene_186692 Copy
Species: Synthetic
Genetic Insert: sYFP2
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5139; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201004 Copy
Species: Synthetic
Genetic Insert: sYFP2
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5442; Vector Backbone:pTn7-SCOUT21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201008 Copy
Species: Synthetic
Genetic Insert: mcherry
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5442; Vector Backbone:pTn7-SCOUT21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201007 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5442; Vector Backbone:pTn7-SCOUT20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200995 Copy
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