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Species: Methanosarcina mazei
Genetic Insert: 4 copies of pyrrolysyl-tRNA mutant PyOtR
Vector Backbone Description: Backbone Marker:derived from pFastBac-Dual from Invitrogen.; Backbone Size:8024; Vector Backbone:pAcBac1; Vector Types:Mammalian Expression, Insect Expression, baculoviral; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38279536
Proper citation: RRID:Addgene_217364 Copy
Species: Homo sapiens
Genetic Insert: CDK7 (S164D, T170E)_del327-346
Vector Backbone Description: Vector Backbone:pFastBac dual; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38870939
Proper citation: RRID:Addgene_217012 Copy
Genetic Insert: Enhanced green fluorescent protein
Vector Backbone Description: Backbone Marker:derived from pFastBac-Dual from Invitrogen.; Backbone Size:7942; Vector Backbone:pAcBac1-EGFP; Vector Types:Mammalian Expression, Insect Expression, baculoviral; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:36550276
Comments: the 39 amino acid position for Y39TAG is relative to the fully processed eGFP (i.e. the numbering for amino acid position starts after the methionine start codon that is removed from eGFP post-translationally).
Proper citation: RRID:Addgene_217360 Copy
Species: Other
Genetic Insert: SpyCatcher:mTFP1
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225189 Copy
Species: Other
Genetic Insert: SpyCatcher:mKate2
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225188 Copy
Species: Other
Genetic Insert: SpyCatcher:AausFP1
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225186 Copy
Species: Other
Genetic Insert: SpyCatcher:mNeonGreen
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225185 Copy
Species: Other
Genetic Insert: PrecA:mNeonGreen
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225192 Copy
Species: Other
Genetic Insert: SnoopCatcher:mNeonGreen
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225191 Copy
Species: Other
Genetic Insert: SpyCatcher003:mNeonGreen
Vector Backbone Description: Vector Backbone:pTn7xTS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40251446
Comments: Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
Proper citation: RRID:Addgene_225190 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234583 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234584 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234585 Copy
Species: Homo sapiens
Genetic Insert: EED
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234576 Copy
Species: Synthetic
Genetic Insert: optimized TetR
Vector Backbone Description: Vector Backbone:pUC18T-mini-Tn7T-P3-luxABCDE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40595632
Comments: Please visit https://doi.org/10.1101/2024.09.01.610675 for bioRxiv preprint.
S17-1λpir strain genotype: TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir
Proper citation: RRID:Addgene_226434 Copy
Species: Scytonema hofmanni
Genetic Insert: tnsB
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.
Proper citation: RRID:Addgene_186692 Copy
Species: Synthetic
Genetic Insert: sYFP2
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5139; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201004 Copy
Species: Synthetic
Genetic Insert: sYFP2
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5442; Vector Backbone:pTn7-SCOUT21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201008 Copy
Species: Synthetic
Genetic Insert: mcherry
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5442; Vector Backbone:pTn7-SCOUT21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201007 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5442; Vector Backbone:pTn7-SCOUT20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200995 Copy
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