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Species: Synthetic
Genetic Insert: MSP2N2
Vector Backbone Description: Backbone Size:5387; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Expresses HRV cleavable Avitagged MSP2N2 nanodiscs. Derived from Addgene plasmid: 29520. To be co-expressed with BirA to achieve biotinylation during expression.
Grinkova YV, Denisov IG, Sligar SG. Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers. Protein Eng Des Sel. 2010 Nov;23(11):843-8. doi: 10.1093/protein/gzq060. Epub 2010 Sep 3. PMID: 20817758; PMCID: PMC2953958.
Proper citation: RRID:Addgene_228493 Copy
Species: Synthetic
Genetic Insert: FIRE2 SENSOR
Vector Backbone Description: Vector Backbone:nonspecific; Vector Types:Mammalian Expression, Synthetic Biology, Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39824193
Proper citation: RRID:Addgene_228701 Copy
Species: Synthetic
Genetic Insert: ChrimsonR-tdTomato
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39553945
Comments: Please visit https://doi.org/10.1101/2024.11.01.621418 for bioRxiv preprint.
Proper citation: RRID:Addgene_229775 Copy
Species: Synthetic
Genetic Insert: FIRE1 SENSOR
Vector Backbone Description: Vector Backbone:nonspecific; Vector Types:Mammalian Expression, Synthetic Biology, Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39824193
Proper citation: RRID:Addgene_228697 Copy
Species: Synthetic
Genetic Insert: FIRE1 CONTROL
Vector Backbone Description: Vector Backbone:nonspecific; Vector Types:Mammalian Expression, Synthetic Biology, Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39824193
Proper citation: RRID:Addgene_228698 Copy
Species: Synthetic
Genetic Insert: iCre(R297T)
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Comments: Allen Institute plasmid alias: CN3980
Proper citation: RRID:Addgene_229918 Copy
Species: Synthetic
Genetic Insert: iCre(R297T)
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Comments: Allen Institute plasmid alias: CN4186
Proper citation: RRID:Addgene_229919 Copy
Species: Synthetic
Genetic Insert: Affimer6
Vector Backbone Description: Vector Backbone:pCMVtrunc (Watanabe and Mitchison, 2002); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40179884
Comments: Please visit https://doi.org/10.1101/2024.04.26.591305 for bioRxiv preprint.
This plasmid is also called Af30 in the paper.
Proper citation: RRID:Addgene_231551 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Comments: Allen Institute plasmid alias: CN2317
Proper citation: RRID:Addgene_229884 Copy
Species: Synthetic
Genetic Insert: RMacL3-04.EGFP.BCB1
Vector Backbone Description: Vector Backbone:pEMS2115 (Addgene Plasmid #49140); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403706
Comments: Please visit https://doi.org/10.1101/2024.12.01.626253 for bioRxiv preprint.
Proper citation: RRID:Addgene_225688 Copy
Species: Synthetic
Genetic Insert: RMacL3-03.EGFP.BCA12
Vector Backbone Description: Vector Backbone:pEMS2115 (Addgene Plasmid #49140); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403706
Comments: Please visit https://doi.org/10.1101/2024.12.01.626253 for bioRxiv preprint.
Proper citation: RRID:Addgene_225687 Copy
Species: Synthetic
Genetic Insert: Z3 promoter and Z3EV transcription factor
Vector Backbone Description: Vector Backbone:pCas9-EcRT-GAL-Kan; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40758833
Comments: The GAL1-GAL10 promoter driving expression of SpCas9 and Ec86 RT in pCas9-EcRT-GAL-Kan was swapped with an estradiol-inducible Z3 promoter and the Z3EV transcription factor was inserterted into the Mph1103I site. Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.
Proper citation: RRID:Addgene_232096 Copy
Species: Synthetic
Genetic Insert: TadA-CDd-nCas9-SpG-UGI
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41087678
Comments: Derived from David Liu's Base editing vectors and Keith Joung's SpG-Cas9 variant.
Please visit https://doi.org/10.1101/2024.11.12.622254 for bioRxiv preprint.
Proper citation: RRID:Addgene_231110 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pSEVA471; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39727169
Comments: Please visit https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint.
Proper citation: RRID:Addgene_231419 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38915722
Comments: Cloning Method: RE Cloning
Proper citation: RRID:Addgene_224060 Copy
Species: Synthetic
Genetic Insert: Tornado-1xALFATag-5xSunTag_codon_optimized-8xAAA
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39892379
Proper citation: RRID:Addgene_231604 Copy
Species: Synthetic
Genetic Insert: Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39892379
Proper citation: RRID:Addgene_231603 Copy
Species: Synthetic
Genetic Insert: Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39892379
Proper citation: RRID:Addgene_231592 Copy
Species: Synthetic
Genetic Insert: Tornado-2xALFATag-10xSunTag
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39892379
Proper citation: RRID:Addgene_231591 Copy
Species: Synthetic
Genetic Insert: Kozak sequence-Tornado-10xSunTag-2xALFATag
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39892379
Proper citation: RRID:Addgene_231594 Copy
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