Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Homo sapiens
Genetic Insert: SAMTOR
Vector Backbone Description: Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100520 Copy
Species: Homo sapiens
Genetic Insert: Metap2
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100516 Copy
Species: Homo sapiens
Genetic Insert: SESN2
Vector Backbone Description: Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100519 Copy
Species: Homo sapiens
Genetic Insert: SAMTOR
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100513 Copy
Species: Homo sapiens
Genetic Insert: Metap2
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_100512 Copy
Species: Homo sapiens
Genetic Insert: thymidylate synthetase
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29100421
Proper citation: RRID:Addgene_100545 Copy
Species: Homo sapiens
Genetic Insert: thymidine kinase 1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29100421
Proper citation: RRID:Addgene_100544 Copy
Species: Homo sapiens
Genetic Insert: RARRES3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20100577
Proper citation: RRID:Addgene_100724 Copy
Species: Homo sapiens
Genetic Insert: C-KDEL
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: KDEL sequence at C terminal of the coding sequence cloned in position 7.
Proper citation: RRID:Addgene_100681 Copy
Species: Homo sapiens
Genetic Insert: Pim1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23936194
Proper citation: RRID:Addgene_100722 Copy
Species: Homo sapiens
Genetic Insert: MTMR1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27018598
Comments: The MTMR1 construct is for structural determination and contains the PH-GRAM and PTPase domains (amino acids 95-607). PTPase activity was confirmed.
Proper citation: RRID:Addgene_100723 Copy
Species: Homo sapiens
Genetic Insert: PGKpA
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: mouse phosphoglucokinase polyA. Terminator.
Proper citation: RRID:Addgene_100683 Copy
Species: Homo sapiens
Genetic Insert: Kozak_ATG_Palm
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: N-terminal GAP43-palmitoylation sequence. Petide tag for targeting the protein to the plasma membrane
Proper citation: RRID:Addgene_100694 Copy
Species: Homo sapiens
Genetic Insert: sgSTOP targeting SPRTN (cloned using BbsI)
Vector Backbone Description: Vector Backbone:B52 plasmid expressing SPRTN sgSTOP and containing an empty sgRNA-expression cassette; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28890334
Proper citation: RRID:Addgene_100709 Copy
Species: Homo sapiens
Genetic Insert: sgSTOP targeting TIMELESS (cloned using BbsI)
Vector Backbone Description: Vector Backbone:B52 plasmid expressing TIMELESS sgSTOP and containing an empty sgRNA-expression cassette; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28890334
Proper citation: RRID:Addgene_100713 Copy
Species: Homo sapiens
Genetic Insert: Kozak-ATG-MLS
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Kozak-ATG-MLS (Mitochondrial Localization Sequence)
Proper citation: RRID:Addgene_100676 Copy
Species: Homo sapiens
Genetic Insert: sgSTOP targeting PARP4 (cloned using BbsI)
Vector Backbone Description: Vector Backbone:B52 plasmid expressing PARP4 sgSTOP and containing an empty sgRNA-expression cassette; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28890334
Proper citation: RRID:Addgene_100711 Copy
Species: Homo sapiens
Genetic Insert: sgSTOP targeting CHEK2 (cloned using BbsI)
Vector Backbone Description: Vector Backbone:B52 plasmid expressing CHEK2 sgSTOP and containing an empty sgRNA-expression cassette; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28890334
Proper citation: RRID:Addgene_100712 Copy
Species: Homo sapiens
Genetic Insert: GST-Nix-S212A
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4900; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Use E. coli LB21 for expression
Proper citation: RRID:Addgene_100760 Copy
Species: Homo sapiens
Genetic Insert: GST-Nix-S212D
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4900; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Use E. coli LB21 for expression
Proper citation: RRID:Addgene_100761 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.