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Vector Backbone Description: Backbone Size:7473; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58548 Copy
Vector Backbone Description: Backbone Size:7912; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The sequence discrepancies between the full sequence and Addgene's QC sequence should not have any functional consequences.
Proper citation: RRID:Addgene_58556 Copy
Vector Backbone Description: Backbone Size:8299; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58557 Copy
Vector Backbone Description: Backbone Size:8098; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58550 Copy
Vector Backbone Description: Backbone Size:7084; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58552 Copy
Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: The V5 epitope tag serves as a linker between Dam and your favorite protein.
The lentiviral vectors can be used in two ways. When transfected as a conventional plasmid, the strong viral promoter drives high expression of the Dam(fusion) proteins. This should not be used for DamID experiments, but it is useful for checking of the proteins by Western blotting or immunofluorescence microscopy. When used as lentivirus, the strong promoter is deleted upon integration of the virus, and expression of the Dam(fusion) is now driven by the weak pIND-derived heatshock promoter.
Proper citation: RRID:Addgene_59205 Copy
Vector Backbone Description: Backbone Marker:van Steensal lab, Addgene plasmid 59201; Backbone Size:7660; Vector Backbone:pIND(V5)EcoDam; Vector Types:Mammalian Expression, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: Note that this vector can not be used to express Dam only.
Proper citation: RRID:Addgene_59204 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-cat
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59382 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-cat2
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. The 3'-end was modified from 5'-GGGCGGGGCGTAA to 5'-AGGAGGTGCATAA in order to provide a site resembling the consensus bacterial ribosomal binding site (5'-AGGAGGTAAATAA). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59385 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4283; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24413736
Comments: Gateway cassette was inserted between SacI and XhoI restriction sites in the MCS.
Proper citation: RRID:Addgene_60323 Copy
Vector Backbone Description: Vector Backbone:pPB-PGK; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25215486
Proper citation: RRID:Addgene_60436 Copy
Vector Backbone Description: Backbone Size:7105; Vector Backbone:pDEST-0GWA; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23471584
Proper citation: RRID:Addgene_60556 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8658; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_61600 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7988; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61702 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Luciferase, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62179 Copy
Vector Backbone Description: Backbone Marker:John Doench; Backbone Size:9240; Vector Backbone:pcw107; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25538079
Proper citation: RRID:Addgene_62511 Copy
Vector Backbone Description: Backbone Size:8267; Vector Backbone:pDestTol2CG2 (Tol2Kit#395); Vector Types:CRISPR, Tol2 destination vector for Gateway; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25752963
Comments: Reference for the Tol2Kit:
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy, M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P., and Chien, C.B. (2007). The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236, 3088-3099.
Proper citation: RRID:Addgene_63156 Copy
Species: Homo sapiens
Genetic Insert: Sox2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3900; Vector Backbone:pLP-TRE2; Vector Types:Mammalian Expression, Cre/Lox, donor vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
Proper citation: RRID:Addgene_49392 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4238; Vector Backbone:pGL4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25177895
Proper citation: RRID:Addgene_49349 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49633 Copy
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