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Species: Homo sapiens
Genetic Insert: gRNA16-1
Vector Backbone Description: Vector Backbone:pLentiGuide-Puro-FE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075754
Proper citation: RRID:Addgene_248544 Copy
Species: Homo sapiens
Genetic Insert: gRNA21-1
Vector Backbone Description: Vector Backbone:pLentiGuide-Puro-FE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075754
Proper citation: RRID:Addgene_248549 Copy
Species: Homo sapiens
Genetic Insert: gRNA17-1
Vector Backbone Description: Vector Backbone:pLentiGuide-Puro-FE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075754
Proper citation: RRID:Addgene_248545 Copy
Species: Homo sapiens
Genetic Insert: gRNA18-4
Vector Backbone Description: Vector Backbone:pLentiGuide-Puro-FE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075754
Proper citation: RRID:Addgene_248546 Copy
Species: Homo sapiens
Genetic Insert: gRNA19-3
Vector Backbone Description: Vector Backbone:pLentiGuide-Puro-FE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075754
Proper citation: RRID:Addgene_248547 Copy
Vector Backbone Description: Backbone Size:11102; Vector Backbone:pGREG-505; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41312768
Comments: Renilla and Firefly luciferase are codon optimized for expression in S. cerevisiae. Renilla, test sequence and Firefly products are separated by StopGo (E2A) sequences.
Proper citation: RRID:Addgene_236297 Copy
Species: Homo sapiens
Genetic Insert: PITPNM3
Vector Backbone Description: Vector Backbone:pNeonGreen-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40923975
Comments: The Nir1 sequence corresponds to a rare human allele (17-6455609-G-A (GRCh38) in the gnomAD database) with an A885V missense mutation relative to the reference genome. There is no known phenotype associated with mutation of this residue, and it is not predicted to disrupt membrane interactions. The residue is also not conserved between Nir orthologues.
Proper citation: RRID:Addgene_248112 Copy
Species: Homo sapiens
Genetic Insert: FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41533785
Comments: DD-MAPPER: destabilizing domain universal reporter circuit-based activatable sensor.
Proper citation: RRID:Addgene_248354 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIB1
Vector Backbone Description: Backbone Marker:Dave Turner lab (UMich); Backbone Size:4090; Vector Backbone:PCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_248077 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41308125
Proper citation: RRID:Addgene_224549 Copy
Species: Synthetic
Genetic Insert: FKBP12F36V degron (dTAG system), blasticidin, 2xHA tag
Vector Backbone Description: Vector Backbone:puc19; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41308125
Proper citation: RRID:Addgene_224547 Copy
Species: Synthetic
Genetic Insert: 2xClover
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41308125
Proper citation: RRID:Addgene_224548 Copy
Species: Lachnospiraceae bacterium
Genetic Insert: dLbCas12a
Vector Backbone Description: Vector Backbone:pHAGE-EF1a-IRES-puro; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38965442
Proper citation: RRID:Addgene_236364 Copy
Species: Lachnospiraceae bacterium
Genetic Insert: HyperdLbCas12a
Vector Backbone Description: Vector Backbone:pHAGE-EF1a-IRES-puro; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38965442
Proper citation: RRID:Addgene_236367 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8800; Vector Backbone:pLX_313-EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40666908
Comments: Please visit https://doi.org/10.1101/2025.06.27.661951 for bioRxiv preprint.
Proper citation: RRID:Addgene_248084 Copy
Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8800; Vector Backbone:pLX_313-EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40666908
Comments: Please visit https://doi.org/10.1101/2025.06.27.661951 for bioRxiv preprint.
Proper citation: RRID:Addgene_248085 Copy
Species: Synthetic
Genetic Insert: uTEV3-GSG-t2A-EBFP
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41533785
Proper citation: RRID:Addgene_248360 Copy
Species: Homo sapiens
Genetic Insert: SNAP25B-I192N
Vector Backbone Description: Backbone Size:9124; Vector Backbone:pFUW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33147442
Comments: Human SNAP25B with a silent mutation at residue #3 GAA for GAG [E]
Proper citation: RRID:Addgene_248126 Copy
Species: Homo sapiens
Genetic Insert: gRNA2-2
Vector Backbone Description: Vector Backbone:pLentiGuide-Puro-FE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075754
Proper citation: RRID:Addgene_248524 Copy
Species: Homo sapiens
Genetic Insert: SNAP25B-R59P
Vector Backbone Description: Backbone Size:9124; Vector Backbone:pFUW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33147442
Comments: Human SNAP25B with a silent mutation at residue #3 GAA for GAG [E]
Proper citation: RRID:Addgene_248129 Copy
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