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Species: Homo sapiens
Genetic Insert: eCALWY-4 Zn(II) sensor
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pCMV (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19718032
Proper citation: RRID:Addgene_22236 Copy
Species: Homo sapiens
Genetic Insert: eCALWY-5 Zn(II) sensor
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pCMV (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19718032
Proper citation: RRID:Addgene_22237 Copy
Species: Homo sapiens
Genetic Insert: eCALWY6 Zn(II) sensor
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pCMV (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19718032
Proper citation: RRID:Addgene_22238 Copy
Species: Homo sapiens
Genetic Insert: Adaptor protein containing PH domain, PTB domain and Leucine zipper motif
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17765681
Proper citation: RRID:Addgene_22198 Copy
Genetic Insert: shMediator of DNA damage checkpoint 1
Vector Backbone Description: Backbone Size:7927; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22195 Copy
Species: Homo sapiens
Genetic Insert: SCP2 (super core promoter 2)-CMVenh luciferase
Vector Backbone Description: Backbone Size:5500; Vector Backbone:a MODIFIED version (constructed by us) of Invitrogen’s pRC/CMV; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17124735
Proper citation: RRID:Addgene_22230 Copy
Species: Homo sapiens
Genetic Insert: sh Mediator of DNA damage checkpoint 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2562; Vector Backbone:pENTR1A; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_22192 Copy
Species: Homo sapiens
Genetic Insert: Oculocerebrorenal syndrome of Lowe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17765681
Proper citation: RRID:Addgene_22207 Copy
Species: Homo sapiens
Genetic Insert: PITX3-eGFP-loxP-PGK-Puro-loxP
Vector Backbone Description: Backbone Size:4079; Vector Backbone:N/A; Vector Types:ZFN donor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19680244
Proper citation: RRID:Addgene_22208 Copy
Species: Homo sapiens
Genetic Insert: SA-OCT-GFP-2APuro-PA
Vector Backbone Description: Backbone Size:5468; Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19680244
Proper citation: RRID:Addgene_22209 Copy
Vector Backbone Description: Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18326499
Comments: The hairpin sequence is
5'TGC GAG CAA CAG CAT CCA GTT TCA AGA GAA CTG GAT GCT GTT GCT CGC TTT TTT C 3'
May contain a GFP cassette or a puro cassette.
Proper citation: RRID:Addgene_22203 Copy
Species: Homo sapiens
Genetic Insert: INSR insulin receptor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17563366
Proper citation: RRID:Addgene_22286 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: REV1
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19528320
Comments: The REV1 ORF plus 1050 nucleotides (nt) of its upstream region that include the REV1 promoter and 500nt of the downstream region that include the REV1 transcription terminator was cloned in the vector YCplac111, which carries the LEU2 gene, the autonomous replicating sequence ARS1, and the centromeric CEN4 region.
A BglII restriction site was added by site-directed mutagenesis right before the stop codon of REV1. A 33-mer oligonucleotide duplex containing the HA-coding sequence and a BamHI and a BglII restriction site was inserted into the BglII restriction site, adding an HA tag in fusion with REV1, and regenerating a unique BglII restriction site. This step was repeated three times in order to get four HA tags in fusion with REV1.
Proper citation: RRID:Addgene_22287 Copy
Species: Homo sapiens
Genetic Insert: TFII-I
Vector Backbone Description: Backbone Size:8900; Vector Backbone:pSFG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17052463
Comments: This vector was derived from pSFG-tcLuc (Lindemann et al., 1998).
Proper citation: RRID:Addgene_22200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD5
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18757916
Comments: Mutation abolishes the ubiquitin ligase function
Proper citation: RRID:Addgene_22288 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD5
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18757916
Proper citation: RRID:Addgene_22289 Copy
Species: Homo sapiens
Genetic Insert: hBEN
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11438732
Proper citation: RRID:Addgene_22161 Copy
Species: Homo sapiens
Genetic Insert: human Dynamin 1aa
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11782545
Proper citation: RRID:Addgene_22163 Copy
Species: Homo sapiens
Genetic Insert: calcium-modulating ligand
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19135167
Proper citation: RRID:Addgene_22284 Copy
Species: mixture
Genetic Insert: EGFP-ER
Vector Backbone Description: Backbone Size:6959; Vector Backbone:pMH4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706463
Comments: EGFP(A206K)= 925-1707
Calreticulin sgnalseq.=862-912
KDEL coding seq.=1714-1725
Proper citation: RRID:Addgene_22285 Copy
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