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Genetic Insert: NanoLuc
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26918244
Proper citation: RRID:Addgene_68930 Copy
Genetic Insert: NanoLuc
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26918244
Proper citation: RRID:Addgene_68941 Copy
Vector Backbone Description: Vector Backbone:pALC2073; Vector Types:Bacterial Expression, tet inducible E. coli/Staphylococcus shuttle vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18996145
Comments: Note--MCS is inverted in the article describing this plasmid. Addgene's sequencing results with the pRMC2 SEQF primer find the MCS is duplicated, presumably due to the cloning strategy.
Proper citation: RRID:Addgene_68940 Copy
Species: Homo sapiens
Genetic Insert: MTOR-E1799K
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24631838
Comments: Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab
Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.
Proper citation: RRID:Addgene_69009 Copy
Species: Homo sapiens
Genetic Insert: MTOR-C1483F
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24631838
Comments: Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab
Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.
Proper citation: RRID:Addgene_69008 Copy
Species: Rattus norvegicus
Genetic Insert: Gja1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Addition of a fluorescent protein tag to the carboxyl-terminus of Cx43 has been shown to eliminate or attenuate interactions with ZO-1 and Occludin in cell culture. Other binding interactions and channel gating properties may be affected.
Proper citation: RRID:Addgene_69007 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24928858
Proper citation: RRID:Addgene_69004 Copy
Species: Homo sapiens
Genetic Insert: optineurin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24683533
Comments: Wild type construct is pOPTN-EGFP (Plasmid #27052)
Proper citation: RRID:Addgene_68847 Copy
Species: Homo sapiens
Genetic Insert: MTOR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24631838
Comments: Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab
Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.
Proper citation: RRID:Addgene_69013 Copy
Species: Homo sapiens
Genetic Insert: MTOR-F1888L
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24631838
Comments: Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab
Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.
Proper citation: RRID:Addgene_69010 Copy
Species: Homo sapiens
Genetic Insert: Cyclin-Dependent Kinase 19
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8877; Vector Backbone:pLVX-EF1α-IRES-ZsGreen1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26416749
Proper citation: RRID:Addgene_68859 Copy
Species: Homo sapiens
Genetic Insert: Cyclin-Dependent Kinase 19
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8892; Vector Backbone:pLVX-EF1α-IRES-ZsGreen; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26416749
Proper citation: RRID:Addgene_68858 Copy
Species: Homo sapiens
Genetic Insert: cyclin-dependent kinase 8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8880; Vector Backbone:pLVX-EF1α-IRES-mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26416749
Proper citation: RRID:Addgene_68856 Copy
Species: HCV, Aequorea victoria
Genetic Insert: YFP-SMASh
Vector Backbone Description: Backbone Size:6622; Vector Backbone:pCS6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26214256
Proper citation: RRID:Addgene_68853 Copy
Genetic Insert: lox-BFP-loxp
Vector Backbone Description: Vector Backbone:pBSII; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25119047
Proper citation: RRID:Addgene_68972 Copy
Genetic Insert: NanoLuc
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26918244
Proper citation: RRID:Addgene_68907 Copy
Genetic Insert: NanoLuc
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26918244
Proper citation: RRID:Addgene_68904 Copy
Genetic Insert: NanoLuc
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26918244
Proper citation: RRID:Addgene_68902 Copy
Genetic Insert: NanoLuc
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26918244
Proper citation: RRID:Addgene_68909 Copy
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26918244
Comments: Please note that the Addgene verified integrase sequence differs from the depositor's reference sequence but is a known variant (NCBI WP_004291332.1).
Proper citation: RRID:Addgene_68900 Copy
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