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Plasmids are provided by Addgene and DGRC.

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pUC-M1
 
Resource Report
Resource Website 		RRID:Addgene_246824 M1 RNA of E. coli RNase P E. coli Ampicillin PMID:40858540 When using this plasmid, please also cite the original paper: Fukunaga J, Gouda M, Umeda K, Ohno S, Yokogawa T, Nishikawa K. Use of RNase P for efficient preparation of yeast tRNATyr transcript and its mutants. J Biochem. 2006 Jan;139(1):123-7. doi: 10.1093/jb/mvj005. PMID: 16428327 Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:25:44 0
Neo-A OR T OR C-SpyTag
 
Resource Report
Resource Website 		RRID:Addgene_246948 Please see depositor comments below Synthetic Kanamycin PMID:41211854 Full gene insert: MGSS-Neo-GGS(x3)-2A9c-GGS(x3)-TEVc-GGS(x3)-HRV-3Cc-GGS(x3)-SpyTag-GGS(x3)-6xHis Vector Backbone:pET 29b (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
cwby0036
 
Resource Report
Resource Website 		RRID:Addgene_247386 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
cwby0070
 
Resource Report
Resource Website 		RRID:Addgene_247420 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:45 0
cwby0038
 
Resource Report
Resource Website 		RRID:Addgene_247388 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
cwby0035
 
Resource Report
Resource Website 		RRID:Addgene_247385 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
p3360_KanR_2p
 
Resource Report
Resource Website 		RRID:Addgene_247302 AtCHI Arabidopsis thaliana Kanamycin The gene sequences (AtCHI, AtCHS, Sc4CL and AtPAL were obtained from JBEI - ICE -Inventory of composable elements and the plasmids were based on the publication: Carbonell, P., Jervis, A.J., Robinson, C.J. et al. An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals. Commun Biol 1, 66 (2018). https://doi.org/10.1038/s42003-018-0076-9 The Linkers used for the BASIC assembly were kindly provided by Alicia Da Silva and Jean-Loup Faulon at Micalis, INRAE, France. Backbone Marker:Twist Bioscience; Vector Backbone:pTwist_High copy; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
cwby0073
 
Resource Report
Resource Website 		RRID:Addgene_247423 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:45 0
cwby0076
 
Resource Report
Resource Website 		RRID:Addgene_247426 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:45 0
cwby0042
 
Resource Report
Resource Website 		RRID:Addgene_247392 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
cwby0047
 
Resource Report
Resource Website 		RRID:Addgene_247397 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
p3387_KanR_4p
 
Resource Report
Resource Website 		RRID:Addgene_247310 AtCHI Arabidopsis thaliana Kanamycin The gene sequences (AtCHI, AtCHS, Sc4CL and AtPAL were obtained from JBEI - ICE -Inventory of composable elements and the plasmids were based on the publication: Carbonell, P., Jervis, A.J., Robinson, C.J. et al. An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals. Commun Biol 1, 66 (2018). https://doi.org/10.1038/s42003-018-0076-9 The Linkers used for the BASIC assembly were kindly provided by Alicia Da Silva and Jean-Loup Faulon at Micalis, INRAE, France. Backbone Marker:Twist Bioscience; Vector Backbone:pTwist_High copy; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
cwby0048
 
Resource Report
Resource Website 		RRID:Addgene_247398 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
pB06_3360_6p
 
Resource Report
Resource Website 		RRID:Addgene_247311 AtCHI Arabidopsis thaliana Kanamycin The gene sequences (AtCHI, AtCHS, Sc4CL and AtPAL were obtained from JBEI - ICE -Inventory of composable elements and the plasmids were based on the publication: Carbonell, P., Jervis, A.J., Robinson, C.J. et al. An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals. Commun Biol 1, 66 (2018). https://doi.org/10.1038/s42003-018-0076-9 The Linkers used for the BASIC assembly were kindly provided by Alicia Da Silva and Jean-Loup Faulon at Micalis, INRAE, France. Vector Backbone:pBO6; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
cwby0049
 
Resource Report
Resource Website 		RRID:Addgene_247399 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
pET28-hisSumo-KE-Des27.9
 
Resource Report
Resource Website 		RRID:Addgene_248004 KE-Des27.9 Synthetic Kanamycin PMID:40533551 Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:45 0
cwby0083
 
Resource Report
Resource Website 		RRID:Addgene_247433 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:45 0
cwby0045
 
Resource Report
Resource Website 		RRID:Addgene_247395 ccdb Synthetic Kanamycin Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint. Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
pLN051
 
Resource Report
Resource Website 		RRID:Addgene_247314 PodJ Caulobacter crescentus Kanamycin PMID:36971555 pXYFPN-2-PPodJ-PodJ Vector Backbone:pXYFPN; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0
pLN058
 
Resource Report
Resource Website 		RRID:Addgene_247316 PodJΔIDR Caulobacter crescentus Kanamycin PMID:36971555 pXYFPN-2-PPodJ-PodJΔIDR Vector Backbone:pXYFPN; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:25:44 0

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