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Species: Homo sapiens
Genetic Insert: Myc shRNA
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRetrosuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17558397
Comments: hairpin targets the sequence: GATGAGGAAGAAATCGATG
Proper citation: RRID:Addgene_15662 Copy
Species: Synthetic
Genetic Insert: Nsp10
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: GAGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQSADAQSFLNGFAVx
Proper citation: RRID:Addgene_156479 Copy
Species: Synthetic
Genetic Insert: Nsp7
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: GQSKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQx
Proper citation: RRID:Addgene_156476 Copy
Species: Synthetic
Genetic Insert: Nsp9
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: GNNELSPVALRQMSCAAGTTQTACTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELE PPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQx
Proper citation: RRID:Addgene_156478 Copy
Species: Synthetic
Genetic Insert: Nsp8
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: GAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQx
Proper citation: RRID:Addgene_156477 Copy
Species: Synthetic
Genetic Insert: Nsp3c_SUD-C
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: MDSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLR
Proper citation: RRID:Addgene_156471 Copy
Species: Synthetic
Genetic Insert: Nsp3e_NAB
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: GKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETx
Proper citation: RRID:Addgene_156473 Copy
Species: Homo sapiens
Genetic Insert: p15 4xSBR2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pGL2-E1bTATA; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16959612
Comments: SBR2 is one of the Smad Binding Regions on the p15INK4b promoter
Proper citation: RRID:Addgene_15708 Copy
Species: Synthetic
Genetic Insert: Nsp15
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: GLQSLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLx
Proper citation: RRID:Addgene_156481 Copy
Species: Mus musculus
Genetic Insert: M71->P2-IRES-tauLacZ LNL 129 TV
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:high copy number; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15186781
Proper citation: RRID:Addgene_15647 Copy
Species: Homo sapiens
Genetic Insert: TIF1 gamma
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751102
Proper citation: RRID:Addgene_15734 Copy
Species: Homo sapiens
Genetic Insert: Smad2 (L+MH2)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751102
Proper citation: RRID:Addgene_15730 Copy
Species: Mus musculus
Genetic Insert: p110 delta
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pPyCAG-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12774123
Proper citation: RRID:Addgene_15691 Copy
Species: Homo sapiens
Genetic Insert: Fibroblast growth factor 2
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pHis; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157657 Copy
Vector Backbone Description: Backbone Size:4816; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.109, Ligation number L3609.
Proper citation: RRID:Addgene_1574 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GFP-gRNA
Vector Backbone Description: Backbone Size:1861; Vector Backbone:pTPGI; Vector Types:Insert storage (replicative in E. coli); Bacterial Resistance:Ampicillin
Defining Citation: PMID:33191917
Proper citation: RRID:Addgene_157656 Copy
Vector Backbone Description: Backbone Size:4816; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.111, Ligation number L3610.
Proper citation: RRID:Addgene_1575 Copy
Vector Backbone Description: Backbone Size:6921; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.114, Ligation number L3611.
Proper citation: RRID:Addgene_1576 Copy
Vector Backbone Description: Backbone Marker:Arbor Biosciences; Backbone Size:2536; Vector Backbone:pTXTL-P70a(2)-deGFP; Vector Types:Bacterial Expression, Cell-free Expression (E. coli sigma-70); Bacterial Resistance:Ampicillin
Defining Citation: PMID:36427151
Comments: The annotated sequence is also available at the Haynes lab Benchling site: https://benchling.com/s/seq-4Syx826a0gonKiH8Evvg.
Please visit https://doi.org/10.17504/protocols.io.brgcm3sw for the protocol.
Proper citation: RRID:Addgene_157652 Copy
Species: Homo sapiens
Genetic Insert: Apoptosis inhibitor 5
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157655 Copy
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