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Species: Homo sapiens
Genetic Insert: TIF1 gamma shRNA
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pRetrosuper-GFP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751102
Comments: shRNA oligos were first cloned into pRetrosuper-Puro digested with BglII and HindIII.
Next, the AgeI/XhoI fragments from shRNA sequence-containing pRetrosuper-Puro plasmids were cloned into the AgeI/XhoI site of pRetrosuper-GFP.
Sense oligo: 5'-gatcccc GAAGATGTCT CAGAGTCTG ttcaagaga CAGACTCTGA GACATCTTC tttttggaaa-3'.
Proper citation: RRID:Addgene_15721 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16894141
Comments: The human YAP ORF was cloned into the pBABEpuro vector as an EcoRI–BamHI fragment. A single FLAG tag was added to the N terminus during PCR with the following primers: Hs.YAP.F, CCGGGATCCACCATGGATTACAAGGATGACGACGATAAGATGGACCCCGGGCAGCAGCCCGCCGC; and Hs.YAP.R, CCGGAATTCCTATAACCATGTAAGAAAGCTTTC.
Proper citation: RRID:Addgene_15682 Copy
Species: Rattus norvegicus
Genetic Insert: PHAS-I F113A
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4671; Vector Backbone:pET14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12665511
Comments: Full-length PHASI with a His tag. See author's map for detailed information.
Proper citation: RRID:Addgene_15680 Copy
Species: Rattus norvegicus
Genetic Insert: rApoI
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:29991261
Comments: Esherichia coli strain that expresses rat APOBEC1. Genotype: DH10B Δung ΔmotAB ΔcsgABCDEFG [PlacI<>Ptac] Δ(araA-leu)7697::[PA1lacO-Tenth rApo1]
Proper citation: RRID:Addgene_156459 Copy
Species: Tn5
Genetic Insert: NeoR/KanR
Vector Backbone Description: Backbone Size:5863; Vector Backbone:BAC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29991261
Comments: Please note that a low DNA yield is expected with single copy BACs. Using larger scale DNA preps (ie. midi preps) is recommended. If there are difficulties removing genomic DNA, the ExonucleaseV (recBCD) from NEB (M0345S) which degrades contaminating linear DNA can help improve results.
Proper citation: RRID:Addgene_156454 Copy
Vector Backbone Description: Backbone Size:6921; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.70, Ligation number L3574.
Proper citation: RRID:Addgene_1566 Copy
Species: Escherichia coli
Genetic Insert: rpsL
Vector Backbone Description: Backbone Size:4732; Vector Backbone:BAC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29991261
Comments: Please note that a low DNA yield is expected with single copy BACs. Using larger scale DNA preps (ie. midi preps) is recommended. If there are difficulties removing genomic DNA, the ExonucleaseV (recBCD) from NEB (M0345S) which degrades contaminating linear DNA can help improve results.
Proper citation: RRID:Addgene_156455 Copy
Vector Backbone Description: Backbone Size:4816; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.99, Ligation number L3604.
Proper citation: RRID:Addgene_1569 Copy
Species: Homo sapiens
Genetic Insert: TIF1 gamma shRNA
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRetrosuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751102
Comments: shRNA oligos were cloned into pRetrosuper-Puro digested with BglII and HindIII.
Sense oligo: 5'-gatcccc GAAGATGTCT CAGAGTCTG ttcaagaga CAGACTCTGA GACATCTTC tttttggaaa-3'.
Proper citation: RRID:Addgene_15728 Copy
Species: Mus musculus
Genetic Insert: HybridJ-IRES-tauGFP LNL TV
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:high copy number; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15186781
Comments: The mouse strain that was derived from this plasmid is available from The Jackson Laboratory: http://jaxmice.jax.org/strain/006622.html
Proper citation: RRID:Addgene_15658 Copy
Species: Mus musculus
Genetic Insert: HybridH-IRES-tauGFP LNL TV
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:high copy number; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15186781
Comments: The mouse strain that was derived from this plasmid is available from The Jackson Laboratory: http://jaxmice.jax.org/strain/006624.html
Proper citation: RRID:Addgene_15656 Copy
Species: Mus musculus
Genetic Insert: HybridF-IRES-tauGFP LNL TV
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:high copy number; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15186781
Comments: The mouse strain that was derived from this plasmid is available from The Jackson Laboratory: http://jaxmice.jax.org/strain/006626.html
Proper citation: RRID:Addgene_15654 Copy
Species: Mus musculus
Genetic Insert: HybridB-IRES-tauLacZ LNL TV
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:high copy number; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15186781
Comments: The mouse strain that was derived from this plasmid is available from The Jackson Laboratory: http://jaxmice.jax.org/strain/006629.html
Proper citation: RRID:Addgene_15652 Copy
Species: Rattus norvegicus
Genetic Insert: rApoI
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:29991261
Comments: Esherichia coli strain that expresses MutaT7, a fusion of rat APOBEC1 and the T7 RNA polymerase. Genotype: DH10B Δung ΔmotAB ΔcsgABCDEFG [PlacI<>Ptac] Δ(araA-leu)7697::[PA1lacO-Tenth MutaT7]
Proper citation: RRID:Addgene_156460 Copy
Species: Mus musculus
Genetic Insert: Intercellular Adhesion Molecule 1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4179; Vector Backbone:pFUSE; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32783949
Comments: Depositor confirms P66S and L234P mutations does not affect plasmid function.
Proper citation: RRID:Addgene_156462 Copy
Species: Mus musculus
Genetic Insert: Rab38
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6400; Vector Backbone:pcDNA-DEST53 Gateway; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11917121
Proper citation: RRID:Addgene_15669 Copy
Species: Homo sapiens
Genetic Insert: USP28 shRNA
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRetrosuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17558397
Comments: hairpin targets the sequence: GTATGGACAAGAGCGTTGGT
Proper citation: RRID:Addgene_15664 Copy
Species: Homo sapiens
Genetic Insert: USP28 shRNA
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRetrosuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17558397
Comments: Hairpin targets the sequence: GGAGTGAGATTGAACAAGA.
Proper citation: RRID:Addgene_15663 Copy
Species: Homo sapiens
Genetic Insert: Myc shRNA
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRetrosuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17558397
Comments: hairpin targets the sequence: GATGAGGAAGAAATCGATG
Proper citation: RRID:Addgene_15662 Copy
Species: Synthetic
Genetic Insert: Nsp10
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: GAGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQSADAQSFLNGFAVx
Proper citation: RRID:Addgene_156479 Copy
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