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Species: Synthetic
Genetic Insert: FLIM-AKAR
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5100; Vector Backbone:AAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24765076
Proper citation: RRID:Addgene_60445 Copy
Species: Synthetic
Genetic Insert: FLIM-AKART391A
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5100; Vector Backbone:AAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24765076
Proper citation: RRID:Addgene_60446 Copy
Species: Synthetic
Genetic Insert: DAAO
Vector Backbone Description: Backbone Size:3982; Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24020354
Proper citation: RRID:Addgene_60344 Copy
Species: Synthetic
Genetic Insert: GFPpA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS KS+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Comments: This construct is modified pGOF18 from Young Young Il Yeom et al.,Development 122, 000-000 (1996) 881.
Proper citation: RRID:Addgene_60527 Copy
Species: Synthetic
Genetic Insert: mTurquoise
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20081836
Proper citation: RRID:Addgene_60559 Copy
Species: Synthetic
Genetic Insert: mTurquoise
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20081836
Proper citation: RRID:Addgene_60558 Copy
Species: Synthetic
Genetic Insert: Reporter_9
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60569 Copy
Species: Synthetic
Genetic Insert: Reporter_8
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60568 Copy
Species: Synthetic
Genetic Insert: Reporter_7
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60567 Copy
Species: Synthetic
Genetic Insert: Hygromycin resistant gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3755; Vector Backbone:pENTR2B; Vector Types:Mammalian Expression, Knock-in donor vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25434822
Proper citation: RRID:Addgene_60605 Copy
Species: Synthetic
Genetic Insert: Reporter_12
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60572 Copy
Species: Synthetic
Genetic Insert: Cis_7+8+10
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60588 Copy
Species: Synthetic
Genetic Insert: Cis_7+8
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60587 Copy
Species: Synthetic
Genetic Insert: Cis_2+5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60586 Copy
Species: Synthetic
Genetic Insert: MemoryArray
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: An array was constructed by concatenating the attB/P sites of the 11 phage integrases to form a linear 2kb piece of DNA. Random spacers (50 bp) with 50% GC content are included between the att sites.
Proper citation: RRID:Addgene_60585 Copy
Species: Synthetic
Genetic Insert: Cis_2+7+8+5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60589 Copy
Species: Synthetic
Genetic Insert: Cas_5+7_gfp
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60595 Copy
Species: Synthetic
Genetic Insert: Spec
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60593 Copy
Species: Synthetic
Genetic Insert: N Terminal fragment of T7 RNAP split betwen 179-180
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23479654
Proper citation: RRID:Addgene_60720 Copy
Species: Synthetic
Genetic Insert: Residues 1-179 of T7 RNAP
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23479654
Proper citation: RRID:Addgene_60721 Copy
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