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Species: Arabidopsis thaliana
Genetic Insert: promotor SESA3/ AtS2S3 (At4g27160; 550bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105371 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor PAD4 (At3g52430)
Vector Backbone Description: Vector Backbone:pAGM1251 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105367 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor EDS1 (At3g48090)
Vector Backbone Description: Vector Backbone:pAGM1251 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105365 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor (At1g66850;350bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105363 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor ICU2 (At5g67100;600bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105361 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor GILT (At4g12960;2100bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105362 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100281 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Proper citation: RRID:Addgene_100537 Copy
Species: Arabidopsis thaliana
Genetic Insert: OEP7(1–50)-Citrine
Vector Backbone Description: Vector Backbone:pMpGWB106; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28634853
Proper citation: RRID:Addgene_100602 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-LS-F138I-T78H
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100963 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-LS-L255I
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100964 Copy
Species: Arabidopsis thaliana
Genetic Insert: PYR1
Vector Backbone Description: Backbone Size:1774; Vector Backbone:pYTK084; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35726092
Proper citation: RRID:Addgene_183176 Copy
Species: Arabidopsis thaliana
Genetic Insert: 5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
Vector Backbone Description: Backbone Size:2710; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Submitted the 5X Myc-Spacers+Degron sequence (see below) to Genscript for GenePlus Economy synthesis and cloning into the EcoRV site of pUC57.
Proper citation: RRID:Addgene_110913 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29859829
Proper citation: RRID:Addgene_111507 Copy
Species: Arabidopsis thaliana
Genetic Insert: IAA17
Vector Backbone Description: Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112034 Copy
Species: Arabidopsis thaliana
Genetic Insert: iLOV
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060199
Proper citation: RRID:Addgene_26587 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2-CreN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pMCherryC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21037589
Proper citation: RRID:Addgene_26888 Copy
Species: Arabidopsis thaliana
Genetic Insert: SPL11 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCGTAG; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: SPL11p::NLS-GFP was generated by PCR amplification of 2.2-kb genomic DNA. The resulting amplicon was then cloned into pCR8/GW-TOPO (Invitrogen) and recombined with pCGTAG.
The primers used to create this construct were 5'-AAATCTCGTCCAACT-3' and 5'-TCACGATATGGGTTG-3'.
Proper citation: RRID:Addgene_27411 Copy
Species: Arabidopsis thaliana
Genetic Insert: SPL11 mutant
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBIB-KAN-GW; Vector Types:used to create transgenic lines; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: Created by cloning 5.5-kb (including 2.7 kb upstream of
CDS, CDS, and 1.3 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). The Quick-
Change Lightning Site-Directed Mutagenesis kit (Stratagene) was used on the resulting plasmids to disrupt miR156-binding sites (primers 5'-AATCTCAAGATATCCACCGTGCACTGTCATTGCTCTCAACCTCTTCGGATCCCCTGG-3' and 5'-CCAGGGGATCCGAAGAGGTTGAGAGCAATGACAGTGCACGGTGGATATCTTGAGATT-3').
This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen).
Proper citation: RRID:Addgene_27387 Copy
Species: Arabidopsis thaliana
Genetic Insert: SPL10 inverted
Vector Backbone Description: Backbone Size:0; Vector Backbone:UBI3pTpFSPGW-HYGv02; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: SPL10-RNAi constructs were generated by amplifying 0.6kb
of the SPL10 CDS from cDNA prepared from inflorescence tissue total RNA isolated using TRIzol reagent (Invitrogen) and reverse-transcribed with oligo(dT) primers using SuperScript
III Reverse Transcriptase (Invitrogen). The resulting amplicons were then cloned into pCR8/GW-TOPO (Invitrogen) and then recombined with UBI3p::pFSPGW-HYGv02 to create an inverted repeat of the SPL10 cDNA fragment under the control
of the UBI3 promoter. For details regarding the UBI3p::pFSPGW-HYGv02 vector, please see the article.
Proper citation: RRID:Addgene_27409 Copy
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