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pAT28-proD/SaEGFP
 
Resource Report
Resource Website 		RRID:Addgene_120321 eGFP with Staphylococcus aureus codon optimization Synthetic Spectinomycin PMID:28264909 Backbone Marker:Patrick Trieu-Cuot; Vector Backbone:pAT28; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:21 0
5aa SunTag VP64 AP3
 
Resource Report
Resource Website 		RRID:Addgene_115484 g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS Spectinomycin PMID:30760722 Vector Backbone:pMOA34; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:35 0
pMN19
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_11572 CaMV 35S enhancers Spectinomycin PMID:10759496 Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article. Kanamycin gene for plant selection on MS media. Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l). The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration. Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:36 1
pSW646 - pML1>>
 
Resource Report
Resource Website 		RRID:Addgene_115995 pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL47 - pSCR>>
 
Resource Report
Resource Website 		RRID:Addgene_116013 pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL94 - pOp4:VND7
 
Resource Report
Resource Website 		RRID:Addgene_116016 pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL44 - pVND7>>
 
Resource Report
Resource Website 		RRID:Addgene_116010 pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL46 - pWOX4>>
 
Resource Report
Resource Website 		RRID:Addgene_116012 pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pSW554 - pOp5:SP-mVenus-HDEL
 
Resource Report
Resource Website 		RRID:Addgene_116006 pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL41 - pSMXL5>>
 
Resource Report
Resource Website 		RRID:Addgene_116007 pSMXL5:GR-LhG4:tSMXL5:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL43 - pNST3>>
 
Resource Report
Resource Website 		RRID:Addgene_116009 pNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pSW683 - pAHP6>>
 
Resource Report
Resource Website 		RRID:Addgene_115998 pAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pH7m34GW_RcCHAD-mCherry
 
Resource Report
Resource Website 		RRID:Addgene_127784 Ubi10p:RcCHAD-mCherry Ricinus communis Spectinomycin PMID:31133615 Backbone Size:8913; Vector Backbone:pH7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:04:40 0
pCAH01Sp::Cas9D10A
 
Resource Report
Resource Website 		RRID:Addgene_128161 Sp Cas9 S. pyogenes Spectinomycin PMID:30926729 Backbone Size:7755; Vector Backbone:pCAH01SpR; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin D10A 2026-08-15 01:04:43 0
pLZts
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_128799 Spectinomycin PMID:32430813 Backbone Size:3733; Vector Backbone:pLZ12-Spec; Vector Types:Bacterial mutagenesis; Bacterial Resistance:Spectinomycin 2026-08-15 01:04:51 1
pDONR223 ATG3 C264A
 
Resource Report
Resource Website 		RRID:Addgene_129293 Ubiquitin-like-conjugating enzyme ATG3 Homo sapiens Spectinomycin PMID:31315929 Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin changed Cysteine 264 to Alanine 2026-08-15 01:04:56 0
pDONR223 ATG3 allKR
 
Resource Report
Resource Website 		RRID:Addgene_129295 Ubiquitin-like-conjugating enzyme ATG3 Homo sapiens Spectinomycin PMID:31315929 Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin changed all 22 Lysine residues to Arginine 2026-08-15 01:04:56 0
pDONR223 LC3B Q116P G120
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_129291 Microtubule-associated proteins 1A/1B light chain 3B Homo sapiens Spectinomycin PMID:31315929 Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Changed Glutamine 116 to Proline. Deleted amino acids 121-125 (stop codon after G120). 2026-08-15 01:04:55 1
pDONR223 ATG3 WT
 
Resource Report
Resource Website 		RRID:Addgene_129292 Ubiquitin-like-conjugating enzyme ATG3 Homo sapiens Spectinomycin PMID:31315929 Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin 2026-08-15 01:04:56 0
pPSup
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_149416 Spectinomycin PMID:32409318 Backbone Marker:Hellens et al, 2000; Backbone Size:3232; Vector Backbone:pGreen; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:07:14 2

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