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Vector Backbone Description: Backbone Size:7170; Vector Backbone:pEMBL8; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30026278
Proper citation: RRID:Addgene_109320 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Size:5283; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15589161
Proper citation: RRID:Addgene_10921 Copy
Vector Backbone Description: Backbone Size:6967; Vector Backbone:pEMBL8; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30619914
Proper citation: RRID:Addgene_109314 Copy
Species: Rattus norvegicus
Genetic Insert: neuNT
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pSV2 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2871941
Comments: See author's map. The neu cDNA was inserted between the HindIII and SmaI sites of pSV2-neo, removing the neo gene. Has normal neu cDNA as in pSV2 neuN with transforming sequence (1899-2387) of pSV2 neuT. Expressed in NIH cells at higher levels than pSV2 neuT.
Proper citation: RRID:Addgene_10918 Copy
Species: Rattus norvegicus
Genetic Insert: neuN
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pSV2 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2871941
Comments: See author's map. The neu cDNA was inserted between the HindIII and SmaI sites of pSV2-neo, removing the neo gene. Expresses better than pSV2 neuT. Has about 100 nt of 5' untranslated region.
Proper citation: RRID:Addgene_10917 Copy
Species: Homo sapiens
Genetic Insert: p16 INK4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7603984
Comments: A human pl6ink4 cDNA was obtained by PCR amplification of a HeLa cell cDNA library with a 5' primer (5'-GGAATTCACCACCATGGAGCCTTCGGCTGAC-3') and a 3' primer (5'-GGAATTCTCGAGTCAATCGGGGATATCTGAGGGACC-3'). A 472-bp fragment was amplified, purified on a low-melting agarose gel, and cloned directly into pGEM-T (Promega). The resulting plasmid was then used to isolate an EcoRI/Xho I fragment containing the pl6ink4 coding region, which was cloned into pcDNA3 (Invitrogen) to construct pCMV.pl6ink4.
The insert corresponds to isoform 1 but is missing the first 8 amino acids.
Proper citation: RRID:Addgene_10916 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Size:3082; Vector Backbone:pKM464; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating deletion of mycobacterial target genes, using zeocin as a resistance marker.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_109301 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109233 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109253 Copy
Species: Homo sapiens
Genetic Insert: GαS
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109350 Copy
Species: Homo sapiens
Genetic Insert: Cyclin E1
Vector Backbone Description: Vector Backbone:pInducer20; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29148972
Proper citation: RRID:Addgene_109348 Copy
Species: Synthetic
Genetic Insert: ECFP-EYFP FRET positive control
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24108638
Proper citation: RRID:Addgene_109330 Copy
Species: Synthetic
Genetic Insert: anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29915061
Proper citation: RRID:Addgene_109417 Copy
Vector Backbone Description: Backbone Marker:Paul Odgren (Addgene plasmid # 73582); Backbone Size:8411; Vector Backbone:pLenti-CMV-MCs-SV-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_109377 Copy
Species: Other
Genetic Insert: "capacity monitor" (https://www.addgene.org/66073/) has been inserted in the lambda phage integration site.
Vector Backbone Description: Vector Backbone:NCBI RefSeq NC_010473.1 for DH10B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109392 Copy
Species: Synthetic
Genetic Insert: anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29915061
Proper citation: RRID:Addgene_109421 Copy
Species: Homo sapiens
Genetic Insert: Rod opsin
Vector Backbone Description: Backbone Size:5374; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23554393
Comments: Also see Ballister et al. BMC Biol. 2018 (doi: 10.1186/s12915-017-0475-2) for additional use.
Proper citation: RRID:Addgene_109361 Copy
Species: Homo sapiens
Genetic Insert: Gsz chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109355 Copy
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:7636; Vector Backbone:pMLM3705 (Addgene plasmid #47754).; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29524148
Comments: The DNA fragment encoding the effector domain is synthesized by PCR and cloned between the AscI and PacI sites of pdCas9-NED to create in-frame fusion between dCas9 (D10A, H840A) and the effector domain. For experiments involving cell lines stably expressing the dCas9-effector domain fusion protein, the coding sequence of the effector domain can be excised from the pdCas9-NED-based recombinant plasmid and transferred into pHAGE EF1α dCas9-NED (Addgene plasmid #109369). Cloning the AscI-PacI fragment between the MluI and AsiSI sites of pHAGE EF1α dCas9-NED preserves the reading frame of the fusion protein (Goubert et al., 2018).
Proper citation: RRID:Addgene_109358 Copy
Species: Homo sapiens
Genetic Insert: Gsi chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109373 Copy
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