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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pDEST27-GST-ATXN1 FL(84Q)-S776A Resource Report Resource Website |
RRID:Addgene_21758 | Ataxin-1 | Homo sapiens | Ampicillin | PMID:18337722 | Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | insert contains a stretch of 84 Q(Glutamines) and no stop codonSerine 776 changed to Alaninethis is the mutant (expanded) version of Ataxin-1 | Addgene | 2026-10-31 02:07:30 | 0 | |
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pDEST27-GST-ATXN1 FL(30Q)-S776A Resource Report Resource Website |
RRID:Addgene_21756 | Ataxin-1 | Homo sapiens | Ampicillin | PMID:18337722 | Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | insert contains a stretch of 32 amino acids (30 Q interrupted by 2 H) and no stop codon Serine 776 changed to Alanine | Addgene | 2026-10-31 02:07:29 | 0 | |
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pMLM36 (aka pBR-UV5-GP-FD2) Resource Report Resource Website 1+ mentions |
RRID:Addgene_21871 | Ampicillin | PMID:19798082 | Combinatorial libraries of zinc finger arrays made by OPEN are cloned into this plasmid (see Maeder et al., Mol Cel 2008, PMID 18657511). Ligate zinc finger array fragments with BbsI-digested pBR-UV5-GP-FD2 vector backbone to create plasmids that express the zinc finger array as a FLAG-tagged Gal11P fusion in the B2H system. The lacUV5 promoter which drives expression of the Gal11P fusion protein contains a lac operator and therefore can be repressed by lac repressor. This plasmid is a low copy number plasmid and therefore we recommend that minipreps be performed from a 4 to 10 ml culture. Annotations for pMLM36: 12-17: lacUV5 promoter -35 box / 36-41: lacUV5 promoter -10 box / 48: lacUV5 promoter transcription start point / 86-88: Start codon for Gal11P protein fragment / 89-358: Coding sequence for Gal11P a.a. 263-352 (with additional translationally silent mutations) / 407-412: BbsI site #1 / 433-438: BbsI site #2 / 568-1024: f1 phage origin of replication / 1156-2013: beta-lactamase gene cassette (confers resistance to ampicillin and carbenicillin) / 2016-2956: ColE1 origin of replication (plasmid origin) / 3203-3390: rop gene (associated with regulation of copy number) | Backbone Size:3508; Vector Backbone:pMLM36; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:31 | 1 | |||
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Strain CSH100 Resource Report Resource Website |
RRID:Addgene_21875 | CSH100 bacterial Strain | None | PMID:19798082 | F’ lac proA+proB+(lacIq lacPL8)/ara- ∆(gpt-lac)5 This strain will be shipped as bacteria in an LB stab. Upon receipt, requesting scientists should restreak the strain on an M9 minimal plate. After restreaking on M9 to confirm the presence of the F', scientists can grow the strain in liquid LB. M9 minimal medium agar plates: To prepare 500 ml, autoclave 439 ml H2O with 7.5 g Bacto-agar and a stir bar. When agar has cooled to approximately 65°C, add 50 ml 10X M9 salts, 1 ml 1 M MgSO4, 10 ml 20% (w/v) glucose and 0.5 ml 100mM CaCl2 and then pour plates. Plates can be stored indefinitely at 4°C in sealed plastic bags. (Alternatively, M9 plates can be purchased from Teknonva: https://www.teknova.com/content/teknova/us/en/products/product-page.html/m1260.html). | Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-31 02:07:31 | 0 | ||
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Strain KJ1C Resource Report Resource Website |
RRID:Addgene_21874 | KJ1C bacterial Strain | Tetracycline | PMID:19798082 | F- ∆hisB463 ∆(gpt-proAB-arg-lac)XIII zaj::Tn10 | Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Tetracycline | Addgene | 2026-10-31 02:07:31 | 0 | ||
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pMLM292 Resource Report Resource Website 1+ mentions |
RRID:Addgene_21873 | Ampicillin | PMID:20010934 | Zinc fingers are cloned in between the unique XbaI and BamHI sites. This results in an expression vector which encodes a ZFN with the OPEN zinc finger array fused to the mutant FokI nuclease domain. pMLM292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains 235- 822: CMV promoter / 771-775: CAAT box / 804-808: TATA box / 819: 3' end of hCMV / 904-906: ZFN start codon / 910-930: SV40 nuclease localization signal (NLS) / 940- 963: FLAG tag / 964- 969: XbaI site / 980- 985: BamHI site / 986-1573: FokI nuclease domain / 1292&1294: Q486E heterodimer FokI mutation / 1331: I499L heterodimer FokI mutation / 1574-1576: ZFN stop codon / 1686-1910: BGH polyadenylation sequence / 1956-2384: f1 origin / 2411-2720: SV40 early promoter and origin / 2775-2841: EM-7 Promoter / 2842-3240: Blasticidin resistance gene / 3398-3528: SV40 late polyA signal / 3955-4574: pUC origin / 4729-5589: ampicillin (bla) resistance gene (complementary strand) / 5590-5688: bla promoter (complementary strand) | Backbone Size:5725; Vector Backbone:pMLM292; Vector Types:Mammalian Expression, zebrafish expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:31 | 6 | |||
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pcDNA-Dest53-SHP-1 S591A Resource Report Resource Website |
RRID:Addgene_21802 | SHP-1 | Homo sapiens | Ampicillin | PMID:17575265 | Backbone Size:7767; Vector Backbone:pcDNA-Dest53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | changed Serine 591 to Alanine | Addgene | 2026-10-31 02:07:30 | 0 | |
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AIRAP 11: PSMD14.pEYFP.V1 Resource Report Resource Website |
RRID:Addgene_21801 | PSMD14 | Mus musculus | Ampicillin | PMID:16973439 | Note that though there is a Kan resistance gene in this plasmid according to the author's sequence, the gene is not functional. This plasmid does NOT confer Kan resistance. | Backbone Marker:Clontech (?); Backbone Size:4200; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:30 | 0 | |
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AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3 Resource Report Resource Website |
RRID:Addgene_21800 | PSMD14 | Mus musculus | Ampicillin | PMID:16973439 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:30 | 0 | ||
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pET28CLY7 Resource Report Resource Website |
RRID:Addgene_21766 | ECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats | Kanamycin | PMID:17073440 | Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:07:30 | 0 | |||
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pDest472 SHP-1 S591D Resource Report Resource Website |
RRID:Addgene_21806 | SHP-1 | Homo sapiens | Ampicillin | PMID:17575265 | Backbone Size:7420; Vector Backbone:pDest472; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | changed Serine 591 to Aspartic Acid | Addgene | 2026-10-31 02:07:30 | 0 | |
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pDest472 SHP-1 WT Resource Report Resource Website |
RRID:Addgene_21804 | SHP-1 | Homo sapiens | Ampicillin | PMID:17575265 | Backbone Size:7420; Vector Backbone:pDest472; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:30 | 0 | ||
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pET28CLY2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_21761 | ECFP -EYFP linked by flexible linker containing two SGGSGG repeats | Kanamycin | PMID:17073440 | Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:07:30 | 1 | |||
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pET28CLY1 Resource Report Resource Website |
RRID:Addgene_21760 | ECFP -EYFP linked by flexible one SGGSGG repeats | Kanamycin | PMID:17073440 | Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:07:30 | 0 | |||
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IRE1b.WT.9E10.pCDNA3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_21880 | IRE1b | Mus musculus | Ampicillin | Mouse IRE1b, wild-type, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3. | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:07:31 | 1 | ||
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pET28CLY4 Resource Report Resource Website |
RRID:Addgene_21763 | ECFP -EYFP linked by flexible linker containing 4 SGGSGG repeats | Kanamycin | PMID:17073440 | Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:07:30 | 0 | |||
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pET28CLY3 Resource Report Resource Website |
RRID:Addgene_21762 | ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats | Kanamycin | PMID:17073440 | Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:07:30 | 0 | |||
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ATF4 10: 5’ATF4.uORF1.GFP Resource Report Resource Website |
RRID:Addgene_21857 | ATF4 | Mus musculus | Kanamycin | PMID:15479734 | These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin) | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | upstream ORF1 (uORF) of ATF4 fused to EGFP | Addgene | 2026-10-31 02:07:31 | 0 |
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ATF4 9: (SL+)5’ATF4.GFP Resource Report Resource Website |
RRID:Addgene_21856 | ATF4 | Mus musculus | Kanamycin | PMID:15479734 | These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin) | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Stem loop introduced at the 5' end of the mRNA | Addgene | 2026-10-31 02:07:31 | 0 |
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ATF4 8: (SL-)5’ATF4.GFP Resource Report Resource Website |
RRID:Addgene_21855 | ATF4 | Mus musculus | Kanamycin | PMID:15479734 | These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin) | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:07:31 | 0 |
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