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Species: Saccharomyces cerevisiae
Genetic Insert: Mkk2DS NLS mCherry
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26231587
Proper citation: RRID:Addgene_68280 Copy
Species: Synthetic
Genetic Insert: FtCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68326 Copy
Species: Synthetic
Genetic Insert: BcCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68325 Copy
Species: Synthetic
Genetic Insert: LfCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68323 Copy
Species: Synthetic
Genetic Insert: SepRS/EF-Sep, 2xtRNA-Sep-A37
Vector Backbone Description: Vector Backbone:pKD; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26350500
Comments: See supplementary information accompanying article for additional cloning information. We recommend introducing this plasmid into a recoded strain of E. coli (no genomic TAG codons) for high-efficiency phoshoprotein expression.
Proper citation: RRID:Addgene_68289 Copy
Species: Synthetic
Genetic Insert: SpaCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68322 Copy
Species: Synthetic
Genetic Insert: SepRS9/EF-Sep21, 2xtRNA-Sep
Vector Backbone Description: Vector Backbone:pKD; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26350500
Comments: See supplementary information accompanying article for additional cloning information. We recommend introducing this plasmid into a recoded strain of E. coli (no genomic TAG codons) for high-efficiency phoshoprotein expression.
Proper citation: RRID:Addgene_68288 Copy
Species: Synthetic
Genetic Insert: GFP Q157TAG
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26350500
Comments: Encodes reporter protein to assess efficiency of phosphoserine incorporation at TAG codons.
Proper citation: RRID:Addgene_68297 Copy
Species: Synthetic
Genetic Insert: GdCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68330 Copy
Species: Synthetic
Genetic Insert: GFP S2TAG
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26350500
Comments: Encodes reporter protein to assess efficiency of phosphoserine incorporation at TAG codons.
Proper citation: RRID:Addgene_68296 Copy
Species: Synthetic
Genetic Insert: CjCas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68338 Copy
Species: Synthetic
Genetic Insert: St3Cas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68337 Copy
Species: Synthetic
Genetic Insert: ClCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68336 Copy
Species: Synthetic
Genetic Insert: PlCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68333 Copy
Species: Synthetic
Genetic Insert: GFP E17TAG/Q157TAG
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26350500
Comments: Encodes reporter protein to assess efficiency of phosphoserine incorporation at TAG codons.
Proper citation: RRID:Addgene_68299 Copy
Species: Synthetic
Genetic Insert: RiCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68332 Copy
Vector Backbone Description: Backbone Marker:Mosimann lab, Addgene plasmid #68313; Vector Backbone:pDestattB; Vector Types:Zebrafish transgenesis; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23723152
Proper citation: RRID:Addgene_68341 Copy
Species: Synthetic
Genetic Insert: 3xgRNA;PTENA, p53B,SMAD4A
Vector Backbone Description: Backbone Size:2500; Vector Backbone:Amp; Vector Types:Mammalian Expression, AAV, Flp/Frt; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34208747
Proper citation: RRID:Addgene_68346 Copy
Species: Synthetic
Genetic Insert: puromycin
Vector Backbone Description: Backbone Size:2500; Vector Backbone:Amp; Vector Types:Mammalian Expression, CRISPR, Flp/Frt; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34208747
Proper citation: RRID:Addgene_68345 Copy
Species: Homo sapiens
Genetic Insert: EF1a-Cas9 cassette, WPRE
Vector Backbone Description: Backbone Size:5783; Vector Backbone:pKLV2 lentiviral vector; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27760321
Proper citation: RRID:Addgene_68343 Copy
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